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G1-arrested newborn cells are the predominant infectious form of the pathogen Brucella abortus

Several intracellular pathogens, such as Brucella abortus, display a biphasic infection process starting with a non-proliferative stage of unclear nature. Here, we study the cell cycle of B. abortus at the single-cell level, in culture and during infection of HeLa cells and macrophages. The localiza...

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Autores principales: Deghelt, Michaël, Mullier, Caroline, Sternon, Jean-François, Francis, Nayla, Laloux, Géraldine, Dotreppe, Delphine, Van der Henst, Charles, Jacobs-Wagner, Christine, Letesson, Jean-Jacques, De Bolle, Xavier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4104442/
https://www.ncbi.nlm.nih.gov/pubmed/25006695
http://dx.doi.org/10.1038/ncomms5366
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author Deghelt, Michaël
Mullier, Caroline
Sternon, Jean-François
Francis, Nayla
Laloux, Géraldine
Dotreppe, Delphine
Van der Henst, Charles
Jacobs-Wagner, Christine
Letesson, Jean-Jacques
De Bolle, Xavier
author_facet Deghelt, Michaël
Mullier, Caroline
Sternon, Jean-François
Francis, Nayla
Laloux, Géraldine
Dotreppe, Delphine
Van der Henst, Charles
Jacobs-Wagner, Christine
Letesson, Jean-Jacques
De Bolle, Xavier
author_sort Deghelt, Michaël
collection PubMed
description Several intracellular pathogens, such as Brucella abortus, display a biphasic infection process starting with a non-proliferative stage of unclear nature. Here, we study the cell cycle of B. abortus at the single-cell level, in culture and during infection of HeLa cells and macrophages. The localization of segregation and replication loci of the two bacterial chromosomes indicates that, immediately after being engulfed by host-cell endocytic vacuoles, most bacterial cells are newborn. These bacterial cells do not initiate DNA replication for the next 4 to 6 h, indicating a G1 arrest. Moreover, growth is completely stopped during that time, reflecting a global cell cycle block. Growth and DNA replication resume later, although bacteria still reside within endosomal-like compartments. We hypothesize that the predominance of G1-arrested bacteria in the infectious population, and the bacterial cell cycle arrest following internalization, may constitute a widespread strategy among intracellular pathogens to colonize new proliferation niches.
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spelling pubmed-41044422014-07-22 G1-arrested newborn cells are the predominant infectious form of the pathogen Brucella abortus Deghelt, Michaël Mullier, Caroline Sternon, Jean-François Francis, Nayla Laloux, Géraldine Dotreppe, Delphine Van der Henst, Charles Jacobs-Wagner, Christine Letesson, Jean-Jacques De Bolle, Xavier Nat Commun Article Several intracellular pathogens, such as Brucella abortus, display a biphasic infection process starting with a non-proliferative stage of unclear nature. Here, we study the cell cycle of B. abortus at the single-cell level, in culture and during infection of HeLa cells and macrophages. The localization of segregation and replication loci of the two bacterial chromosomes indicates that, immediately after being engulfed by host-cell endocytic vacuoles, most bacterial cells are newborn. These bacterial cells do not initiate DNA replication for the next 4 to 6 h, indicating a G1 arrest. Moreover, growth is completely stopped during that time, reflecting a global cell cycle block. Growth and DNA replication resume later, although bacteria still reside within endosomal-like compartments. We hypothesize that the predominance of G1-arrested bacteria in the infectious population, and the bacterial cell cycle arrest following internalization, may constitute a widespread strategy among intracellular pathogens to colonize new proliferation niches. Nature Pub. Group 2014-07-09 /pmc/articles/PMC4104442/ /pubmed/25006695 http://dx.doi.org/10.1038/ncomms5366 Text en Copyright © 2014, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Article
Deghelt, Michaël
Mullier, Caroline
Sternon, Jean-François
Francis, Nayla
Laloux, Géraldine
Dotreppe, Delphine
Van der Henst, Charles
Jacobs-Wagner, Christine
Letesson, Jean-Jacques
De Bolle, Xavier
G1-arrested newborn cells are the predominant infectious form of the pathogen Brucella abortus
title G1-arrested newborn cells are the predominant infectious form of the pathogen Brucella abortus
title_full G1-arrested newborn cells are the predominant infectious form of the pathogen Brucella abortus
title_fullStr G1-arrested newborn cells are the predominant infectious form of the pathogen Brucella abortus
title_full_unstemmed G1-arrested newborn cells are the predominant infectious form of the pathogen Brucella abortus
title_short G1-arrested newborn cells are the predominant infectious form of the pathogen Brucella abortus
title_sort g1-arrested newborn cells are the predominant infectious form of the pathogen brucella abortus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4104442/
https://www.ncbi.nlm.nih.gov/pubmed/25006695
http://dx.doi.org/10.1038/ncomms5366
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