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A long PCR–based approach for DNA enrichment prior to next-generation sequencing for systematic studies(1)

• Premise of the study: We present an alternative approach for molecular systematic studies that combines long PCR and next-generation sequencing. Our approach can be used to generate templates from any DNA source for next-generation sequencing. Here we test our approach by amplifying complete chlor...

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Detalles Bibliográficos
Autores principales: Uribe-Convers, Simon, Duke, Justin R., Moore, Michael J., Tank, David C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4104715/
https://www.ncbi.nlm.nih.gov/pubmed/25202592
http://dx.doi.org/10.3732/apps.1300063