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Genetic diversity of androdioecious Osmanthus fragrans (Oleaceae) cultivars using microsatellite markers(1)

• Premise of the study: For cultivar classification, identification, and genetic improvement, microsatellite markers were developed to analyze the genetic diversity of androdioecious Osmanthus fragrans cultivars. • Methods and Results: Fifteen microsatellite markers were developed from sequences dow...

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Autores principales: Duan, Yifan, Wang, Xianrong, Xiang, Qibai, Liang, Lili, Li, Xuexia, Liu, Yulian, Li, Meng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105023/
https://www.ncbi.nlm.nih.gov/pubmed/25202550
http://dx.doi.org/10.3732/apps.1200092
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author Duan, Yifan
Wang, Xianrong
Xiang, Qibai
Liang, Lili
Li, Xuexia
Liu, Yulian
Li, Meng
author_facet Duan, Yifan
Wang, Xianrong
Xiang, Qibai
Liang, Lili
Li, Xuexia
Liu, Yulian
Li, Meng
author_sort Duan, Yifan
collection PubMed
description • Premise of the study: For cultivar classification, identification, and genetic improvement, microsatellite markers were developed to analyze the genetic diversity of androdioecious Osmanthus fragrans cultivars. • Methods and Results: Fifteen microsatellite markers were developed from sequences downloaded from the National Center for Biotechnology Information, which included two with null alleles. These primers were screened on 62 typical androdioecious O. fragrans cultivars belonging to four groups (Asiaticus, Albus, Luteus, and Aurantiacus). The number of alleles ranged from two to six, with a mean of 3.7 per locus. The observed and expected heterozygosities ranged from 0.1000 to 0.9091 and from 0.1287 to 0.9167, respectively. Results from structure analyses indicated that Asiaticus and Albus were genetically mixed, and Luteus and Aurantiacus were partially genetically differentiated. • Conclusions: These markers will be useful for genetic study of androdioecious O. fragrans cultivars and facilitate cultivar classification, particularly for the cultivar groups Luteus and Aurantiacus.
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spelling pubmed-41050232014-09-08 Genetic diversity of androdioecious Osmanthus fragrans (Oleaceae) cultivars using microsatellite markers(1) Duan, Yifan Wang, Xianrong Xiang, Qibai Liang, Lili Li, Xuexia Liu, Yulian Li, Meng Appl Plant Sci Primer Note • Premise of the study: For cultivar classification, identification, and genetic improvement, microsatellite markers were developed to analyze the genetic diversity of androdioecious Osmanthus fragrans cultivars. • Methods and Results: Fifteen microsatellite markers were developed from sequences downloaded from the National Center for Biotechnology Information, which included two with null alleles. These primers were screened on 62 typical androdioecious O. fragrans cultivars belonging to four groups (Asiaticus, Albus, Luteus, and Aurantiacus). The number of alleles ranged from two to six, with a mean of 3.7 per locus. The observed and expected heterozygosities ranged from 0.1000 to 0.9091 and from 0.1287 to 0.9167, respectively. Results from structure analyses indicated that Asiaticus and Albus were genetically mixed, and Luteus and Aurantiacus were partially genetically differentiated. • Conclusions: These markers will be useful for genetic study of androdioecious O. fragrans cultivars and facilitate cultivar classification, particularly for the cultivar groups Luteus and Aurantiacus. Botanical Society of America 2013-05-07 /pmc/articles/PMC4105023/ /pubmed/25202550 http://dx.doi.org/10.3732/apps.1200092 Text en © 2013 Botanical Society of America
spellingShingle Primer Note
Duan, Yifan
Wang, Xianrong
Xiang, Qibai
Liang, Lili
Li, Xuexia
Liu, Yulian
Li, Meng
Genetic diversity of androdioecious Osmanthus fragrans (Oleaceae) cultivars using microsatellite markers(1)
title Genetic diversity of androdioecious Osmanthus fragrans (Oleaceae) cultivars using microsatellite markers(1)
title_full Genetic diversity of androdioecious Osmanthus fragrans (Oleaceae) cultivars using microsatellite markers(1)
title_fullStr Genetic diversity of androdioecious Osmanthus fragrans (Oleaceae) cultivars using microsatellite markers(1)
title_full_unstemmed Genetic diversity of androdioecious Osmanthus fragrans (Oleaceae) cultivars using microsatellite markers(1)
title_short Genetic diversity of androdioecious Osmanthus fragrans (Oleaceae) cultivars using microsatellite markers(1)
title_sort genetic diversity of androdioecious osmanthus fragrans (oleaceae) cultivars using microsatellite markers(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105023/
https://www.ncbi.nlm.nih.gov/pubmed/25202550
http://dx.doi.org/10.3732/apps.1200092
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