Cargando…
Development of 13 microsatellite markers in the endangered Sinai primrose (Primula boveana, Primulaceae)(1)
• Premise of the study: We developed microsatellite markers for the endangered plant Primula boveana, the Sinai primrose, and assessed the cross-transferability of these markers to six related taxa. • Methods and Results: DNA sequences containing microsatellites were isolated from a microsatellite-e...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105029/ https://www.ncbi.nlm.nih.gov/pubmed/25202556 http://dx.doi.org/10.3732/apps.1200515 |
_version_ | 1782327303142899712 |
---|---|
author | Mansour, Hassan Jiménez, Ares Keller, Barbara Nowak, Michael D. Conti, Elena |
author_facet | Mansour, Hassan Jiménez, Ares Keller, Barbara Nowak, Michael D. Conti, Elena |
author_sort | Mansour, Hassan |
collection | PubMed |
description | • Premise of the study: We developed microsatellite markers for the endangered plant Primula boveana, the Sinai primrose, and assessed the cross-transferability of these markers to six related taxa. • Methods and Results: DNA sequences containing microsatellites were isolated from a microsatellite-enriched library. We obtained successful amplification of 13 microsatellite primer pairs, seven of which were polymorphic in P. boveana. Eleven of these primers successfully cross-amplified to related taxa. • Conclusions: The markers reported herein will be useful to characterize the genetic diversity of the endangered P. boveana and to evaluate its mating system, and have the potential to be useful for similar studies in close relatives. |
format | Online Article Text |
id | pubmed-4105029 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-41050292014-09-08 Development of 13 microsatellite markers in the endangered Sinai primrose (Primula boveana, Primulaceae)(1) Mansour, Hassan Jiménez, Ares Keller, Barbara Nowak, Michael D. Conti, Elena Appl Plant Sci Primer Note • Premise of the study: We developed microsatellite markers for the endangered plant Primula boveana, the Sinai primrose, and assessed the cross-transferability of these markers to six related taxa. • Methods and Results: DNA sequences containing microsatellites were isolated from a microsatellite-enriched library. We obtained successful amplification of 13 microsatellite primer pairs, seven of which were polymorphic in P. boveana. Eleven of these primers successfully cross-amplified to related taxa. • Conclusions: The markers reported herein will be useful to characterize the genetic diversity of the endangered P. boveana and to evaluate its mating system, and have the potential to be useful for similar studies in close relatives. Botanical Society of America 2013-05-07 /pmc/articles/PMC4105029/ /pubmed/25202556 http://dx.doi.org/10.3732/apps.1200515 Text en © 2013 Botanical Society of America |
spellingShingle | Primer Note Mansour, Hassan Jiménez, Ares Keller, Barbara Nowak, Michael D. Conti, Elena Development of 13 microsatellite markers in the endangered Sinai primrose (Primula boveana, Primulaceae)(1) |
title | Development of 13 microsatellite markers in the endangered Sinai primrose (Primula boveana, Primulaceae)(1) |
title_full | Development of 13 microsatellite markers in the endangered Sinai primrose (Primula boveana, Primulaceae)(1) |
title_fullStr | Development of 13 microsatellite markers in the endangered Sinai primrose (Primula boveana, Primulaceae)(1) |
title_full_unstemmed | Development of 13 microsatellite markers in the endangered Sinai primrose (Primula boveana, Primulaceae)(1) |
title_short | Development of 13 microsatellite markers in the endangered Sinai primrose (Primula boveana, Primulaceae)(1) |
title_sort | development of 13 microsatellite markers in the endangered sinai primrose (primula boveana, primulaceae)(1) |
topic | Primer Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105029/ https://www.ncbi.nlm.nih.gov/pubmed/25202556 http://dx.doi.org/10.3732/apps.1200515 |
work_keys_str_mv | AT mansourhassan developmentof13microsatellitemarkersintheendangeredsinaiprimroseprimulaboveanaprimulaceae1 AT jimenezares developmentof13microsatellitemarkersintheendangeredsinaiprimroseprimulaboveanaprimulaceae1 AT kellerbarbara developmentof13microsatellitemarkersintheendangeredsinaiprimroseprimulaboveanaprimulaceae1 AT nowakmichaeld developmentof13microsatellitemarkersintheendangeredsinaiprimroseprimulaboveanaprimulaceae1 AT contielena developmentof13microsatellitemarkersintheendangeredsinaiprimroseprimulaboveanaprimulaceae1 |