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Development of 13 microsatellite markers in the endangered Sinai primrose (Primula boveana, Primulaceae)(1)

• Premise of the study: We developed microsatellite markers for the endangered plant Primula boveana, the Sinai primrose, and assessed the cross-transferability of these markers to six related taxa. • Methods and Results: DNA sequences containing microsatellites were isolated from a microsatellite-e...

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Autores principales: Mansour, Hassan, Jiménez, Ares, Keller, Barbara, Nowak, Michael D., Conti, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105029/
https://www.ncbi.nlm.nih.gov/pubmed/25202556
http://dx.doi.org/10.3732/apps.1200515
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author Mansour, Hassan
Jiménez, Ares
Keller, Barbara
Nowak, Michael D.
Conti, Elena
author_facet Mansour, Hassan
Jiménez, Ares
Keller, Barbara
Nowak, Michael D.
Conti, Elena
author_sort Mansour, Hassan
collection PubMed
description • Premise of the study: We developed microsatellite markers for the endangered plant Primula boveana, the Sinai primrose, and assessed the cross-transferability of these markers to six related taxa. • Methods and Results: DNA sequences containing microsatellites were isolated from a microsatellite-enriched library. We obtained successful amplification of 13 microsatellite primer pairs, seven of which were polymorphic in P. boveana. Eleven of these primers successfully cross-amplified to related taxa. • Conclusions: The markers reported herein will be useful to characterize the genetic diversity of the endangered P. boveana and to evaluate its mating system, and have the potential to be useful for similar studies in close relatives.
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spelling pubmed-41050292014-09-08 Development of 13 microsatellite markers in the endangered Sinai primrose (Primula boveana, Primulaceae)(1) Mansour, Hassan Jiménez, Ares Keller, Barbara Nowak, Michael D. Conti, Elena Appl Plant Sci Primer Note • Premise of the study: We developed microsatellite markers for the endangered plant Primula boveana, the Sinai primrose, and assessed the cross-transferability of these markers to six related taxa. • Methods and Results: DNA sequences containing microsatellites were isolated from a microsatellite-enriched library. We obtained successful amplification of 13 microsatellite primer pairs, seven of which were polymorphic in P. boveana. Eleven of these primers successfully cross-amplified to related taxa. • Conclusions: The markers reported herein will be useful to characterize the genetic diversity of the endangered P. boveana and to evaluate its mating system, and have the potential to be useful for similar studies in close relatives. Botanical Society of America 2013-05-07 /pmc/articles/PMC4105029/ /pubmed/25202556 http://dx.doi.org/10.3732/apps.1200515 Text en © 2013 Botanical Society of America
spellingShingle Primer Note
Mansour, Hassan
Jiménez, Ares
Keller, Barbara
Nowak, Michael D.
Conti, Elena
Development of 13 microsatellite markers in the endangered Sinai primrose (Primula boveana, Primulaceae)(1)
title Development of 13 microsatellite markers in the endangered Sinai primrose (Primula boveana, Primulaceae)(1)
title_full Development of 13 microsatellite markers in the endangered Sinai primrose (Primula boveana, Primulaceae)(1)
title_fullStr Development of 13 microsatellite markers in the endangered Sinai primrose (Primula boveana, Primulaceae)(1)
title_full_unstemmed Development of 13 microsatellite markers in the endangered Sinai primrose (Primula boveana, Primulaceae)(1)
title_short Development of 13 microsatellite markers in the endangered Sinai primrose (Primula boveana, Primulaceae)(1)
title_sort development of 13 microsatellite markers in the endangered sinai primrose (primula boveana, primulaceae)(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105029/
https://www.ncbi.nlm.nih.gov/pubmed/25202556
http://dx.doi.org/10.3732/apps.1200515
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