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Photoactivatable Glycopolymers for the Proteome-Wide Identification of Fucose-α(1-2)-Galactose Binding Proteins
[Image: see text] Although fucose-α(1-2)-galactose (Fucα(1-2)Gal)-containing glycans have been implicated in cognitive processes such as learning and memory, their precise molecular mechanisms are poorly understood. Here we employed the use of multivalent glycopolymers to enable the first proteome-w...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2014
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105059/ https://www.ncbi.nlm.nih.gov/pubmed/24937314 http://dx.doi.org/10.1021/ja502482a |
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author | Wibowo, Arif Peters, Eric C. Hsieh-Wilson, Linda C. |
author_facet | Wibowo, Arif Peters, Eric C. Hsieh-Wilson, Linda C. |
author_sort | Wibowo, Arif |
collection | PubMed |
description | [Image: see text] Although fucose-α(1-2)-galactose (Fucα(1-2)Gal)-containing glycans have been implicated in cognitive processes such as learning and memory, their precise molecular mechanisms are poorly understood. Here we employed the use of multivalent glycopolymers to enable the first proteome-wide identification of weak affinity, low abundance Fucα(1-2)Gal glycan-binding proteins (GBPs). Biotin-terminated glycopolymers containing photoactivatable cross-linking groups were designed to capture and enrich GBPs from rat brain lysates. Candidate proteins were tested for their ability to bind Fucα(1-2)Gal, and the functional significance of the interaction was investigated for the synaptic vesicle protein SV2a using a knockout mouse system. The results suggest a role for SV2a-Fucα(1-2)Gal interactions in SV2a trafficking and synaptic vesicle recycling. More broadly, our studies outline a general chemical approach for the systems-level discovery of novel GBPs. |
format | Online Article Text |
id | pubmed-4105059 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-41050592014-07-22 Photoactivatable Glycopolymers for the Proteome-Wide Identification of Fucose-α(1-2)-Galactose Binding Proteins Wibowo, Arif Peters, Eric C. Hsieh-Wilson, Linda C. J Am Chem Soc [Image: see text] Although fucose-α(1-2)-galactose (Fucα(1-2)Gal)-containing glycans have been implicated in cognitive processes such as learning and memory, their precise molecular mechanisms are poorly understood. Here we employed the use of multivalent glycopolymers to enable the first proteome-wide identification of weak affinity, low abundance Fucα(1-2)Gal glycan-binding proteins (GBPs). Biotin-terminated glycopolymers containing photoactivatable cross-linking groups were designed to capture and enrich GBPs from rat brain lysates. Candidate proteins were tested for their ability to bind Fucα(1-2)Gal, and the functional significance of the interaction was investigated for the synaptic vesicle protein SV2a using a knockout mouse system. The results suggest a role for SV2a-Fucα(1-2)Gal interactions in SV2a trafficking and synaptic vesicle recycling. More broadly, our studies outline a general chemical approach for the systems-level discovery of novel GBPs. American Chemical Society 2014-06-17 2014-07-09 /pmc/articles/PMC4105059/ /pubmed/24937314 http://dx.doi.org/10.1021/ja502482a Text en Copyright © 2014 American Chemical Society Terms of Use (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) |
spellingShingle | Wibowo, Arif Peters, Eric C. Hsieh-Wilson, Linda C. Photoactivatable Glycopolymers for the Proteome-Wide Identification of Fucose-α(1-2)-Galactose Binding Proteins |
title | Photoactivatable
Glycopolymers for the Proteome-Wide
Identification of Fucose-α(1-2)-Galactose Binding Proteins |
title_full | Photoactivatable
Glycopolymers for the Proteome-Wide
Identification of Fucose-α(1-2)-Galactose Binding Proteins |
title_fullStr | Photoactivatable
Glycopolymers for the Proteome-Wide
Identification of Fucose-α(1-2)-Galactose Binding Proteins |
title_full_unstemmed | Photoactivatable
Glycopolymers for the Proteome-Wide
Identification of Fucose-α(1-2)-Galactose Binding Proteins |
title_short | Photoactivatable
Glycopolymers for the Proteome-Wide
Identification of Fucose-α(1-2)-Galactose Binding Proteins |
title_sort | photoactivatable
glycopolymers for the proteome-wide
identification of fucose-α(1-2)-galactose binding proteins |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105059/ https://www.ncbi.nlm.nih.gov/pubmed/24937314 http://dx.doi.org/10.1021/ja502482a |
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