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MOLECULAR TYPING OF Candida albicans ISOLATES FROM HOSPITALIZED PATIENTS

SUMMARY: Introduction: The majority of nosocomial fungal infections are caused by Candida spp. where C. albicans is the species most commonly identified. Molecular methods are important tools for assessing the origin of the yeasts isolated in hospitals. Methods: This is a study on the genetic profif...

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Autores principales: Bonfim-Mendonça, Patrícia de Souza, Fiorini, Adriana, Shinobu-Mesquita, Cristiane Suemi, Baeza, Lilian Cristiane, Fernandez, Maria Aparecida, Svidzinski, Terezinha Inez Estivalet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto de Medicina Tropical 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105085/
https://www.ncbi.nlm.nih.gov/pubmed/24213190
http://dx.doi.org/10.1590/S0036-46652013000600003
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author Bonfim-Mendonça, Patrícia de Souza
Fiorini, Adriana
Shinobu-Mesquita, Cristiane Suemi
Baeza, Lilian Cristiane
Fernandez, Maria Aparecida
Svidzinski, Terezinha Inez Estivalet
author_facet Bonfim-Mendonça, Patrícia de Souza
Fiorini, Adriana
Shinobu-Mesquita, Cristiane Suemi
Baeza, Lilian Cristiane
Fernandez, Maria Aparecida
Svidzinski, Terezinha Inez Estivalet
author_sort Bonfim-Mendonça, Patrícia de Souza
collection PubMed
description SUMMARY: Introduction: The majority of nosocomial fungal infections are caused by Candida spp. where C. albicans is the species most commonly identified. Molecular methods are important tools for assessing the origin of the yeasts isolated in hospitals. Methods: This is a study on the genetic profifiles of 39 nosocomial clinical isolates of C. albicans using two typing methods: random amplifified polymorphic DNA (RAPD) and microsatellite, two different primers for each technique were used. Results: RAPD provided 10 and 11 different profiles with values for S(AB) of 0.84 ± 0.126 and 0.88 ± 0.08 for primers M2 and P4, respectively. Microsatellite using two markers, CDC3 and HIS3, allowed the observation of six and seven different alleles, respectively, with combined discriminatory power of 0.91. Conclusions: Although genetic variability is clear, it was possible to identify high similarity, suggesting a common origin for at least a part of isolates. It is important to emphasize that common origin was proven from yeasts isolated from colonization (urine, catheter or endotracheal secretions) and blood culture from the same patient, indicating that the candidemia must have started from a site of colonization. The combination of RAPD and microsatellite provides a quick and efficient analysis for investigation of similarity among nosocomial isolates of C. albicans.
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spelling pubmed-41050852014-07-24 MOLECULAR TYPING OF Candida albicans ISOLATES FROM HOSPITALIZED PATIENTS Bonfim-Mendonça, Patrícia de Souza Fiorini, Adriana Shinobu-Mesquita, Cristiane Suemi Baeza, Lilian Cristiane Fernandez, Maria Aparecida Svidzinski, Terezinha Inez Estivalet Rev Inst Med Trop Sao Paulo Mycology SUMMARY: Introduction: The majority of nosocomial fungal infections are caused by Candida spp. where C. albicans is the species most commonly identified. Molecular methods are important tools for assessing the origin of the yeasts isolated in hospitals. Methods: This is a study on the genetic profifiles of 39 nosocomial clinical isolates of C. albicans using two typing methods: random amplifified polymorphic DNA (RAPD) and microsatellite, two different primers for each technique were used. Results: RAPD provided 10 and 11 different profiles with values for S(AB) of 0.84 ± 0.126 and 0.88 ± 0.08 for primers M2 and P4, respectively. Microsatellite using two markers, CDC3 and HIS3, allowed the observation of six and seven different alleles, respectively, with combined discriminatory power of 0.91. Conclusions: Although genetic variability is clear, it was possible to identify high similarity, suggesting a common origin for at least a part of isolates. It is important to emphasize that common origin was proven from yeasts isolated from colonization (urine, catheter or endotracheal secretions) and blood culture from the same patient, indicating that the candidemia must have started from a site of colonization. The combination of RAPD and microsatellite provides a quick and efficient analysis for investigation of similarity among nosocomial isolates of C. albicans. Instituto de Medicina Tropical 2013 /pmc/articles/PMC4105085/ /pubmed/24213190 http://dx.doi.org/10.1590/S0036-46652013000600003 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Mycology
Bonfim-Mendonça, Patrícia de Souza
Fiorini, Adriana
Shinobu-Mesquita, Cristiane Suemi
Baeza, Lilian Cristiane
Fernandez, Maria Aparecida
Svidzinski, Terezinha Inez Estivalet
MOLECULAR TYPING OF Candida albicans ISOLATES FROM HOSPITALIZED PATIENTS
title MOLECULAR TYPING OF Candida albicans ISOLATES FROM HOSPITALIZED PATIENTS
title_full MOLECULAR TYPING OF Candida albicans ISOLATES FROM HOSPITALIZED PATIENTS
title_fullStr MOLECULAR TYPING OF Candida albicans ISOLATES FROM HOSPITALIZED PATIENTS
title_full_unstemmed MOLECULAR TYPING OF Candida albicans ISOLATES FROM HOSPITALIZED PATIENTS
title_short MOLECULAR TYPING OF Candida albicans ISOLATES FROM HOSPITALIZED PATIENTS
title_sort molecular typing of candida albicans isolates from hospitalized patients
topic Mycology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105085/
https://www.ncbi.nlm.nih.gov/pubmed/24213190
http://dx.doi.org/10.1590/S0036-46652013000600003
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