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MiRNA-221 negatively regulated downstream p27Kip1 gene expression involvement in pterygium pathogenesis

PURPOSE: MiRNAs are small noncoding RNAs that have been implicated in tumor development. They regulate target gene expression either by mRNA degradation or by translation repression. Activation of β-catenin has been linked to pterygium progression. Here, we hypothesize that β-catenin-associated miRN...

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Autores principales: Wu, Chueh-Wei, Cheng, Ya-Wen, Hsu, Nan-Yung, Yeh, Ken-Tu, Tsai, Yi-Yu, Chiang, Chun-Chi, Wang, Wei-Ran, Tung, Jai-Nien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Vision 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105113/
https://www.ncbi.nlm.nih.gov/pubmed/25053875
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author Wu, Chueh-Wei
Cheng, Ya-Wen
Hsu, Nan-Yung
Yeh, Ken-Tu
Tsai, Yi-Yu
Chiang, Chun-Chi
Wang, Wei-Ran
Tung, Jai-Nien
author_facet Wu, Chueh-Wei
Cheng, Ya-Wen
Hsu, Nan-Yung
Yeh, Ken-Tu
Tsai, Yi-Yu
Chiang, Chun-Chi
Wang, Wei-Ran
Tung, Jai-Nien
author_sort Wu, Chueh-Wei
collection PubMed
description PURPOSE: MiRNAs are small noncoding RNAs that have been implicated in tumor development. They regulate target gene expression either by mRNA degradation or by translation repression. Activation of β-catenin has been linked to pterygium progression. Here, we hypothesize that β-catenin-associated miRNA, miRNA-221, and downstream p27Kip1 gene expression are correlated with the pathogenesis of pterygium. METHODS: We collected 120 pterygial and 120 normal conjunctival samples for this study. Immunohistochemistry and real-time reverse transcription (RT)–PCR were performed to determine β-catenin protein localization, miR-221, and p27Kip1 gene expression. Pterygium cell line (PECs) cell models were used to confirm the effect of β-catenin, miR-221, and p27Kip1 gene in the proliferation of pterygium cells. RESULTS: Seventy-two (60.0%) pterygial specimens showed high miR-221 expression levels, which was significantly higher than the control groups (13 of 120, 10.8%, p<0.0001). MiR-221 expression was significantly higher in β-catenin-nuclear/cytoplasmic-positive groups than in β-catenin membrane-positive and negative groups (p=0.001). We also found that p27Kip1 gene expression in pterygium was negatively correlated with miR-221 expression (p=0.002). In the clinical association, miR-221 expression was significantly higher in the fleshy and intermediate groups than in the atrophic group (p=0.007). The association of miR-221, p27Kip1 and proliferation of pterygium were also confirmed in the PECs model. CONCLUSIONS: Our study demonstrated that activation of β-catenin in pterygium may interact with miR-221, resulting in p27Kip1 gene downregulation that influences pterygium pathogenesis.
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spelling pubmed-41051132014-07-22 MiRNA-221 negatively regulated downstream p27Kip1 gene expression involvement in pterygium pathogenesis Wu, Chueh-Wei Cheng, Ya-Wen Hsu, Nan-Yung Yeh, Ken-Tu Tsai, Yi-Yu Chiang, Chun-Chi Wang, Wei-Ran Tung, Jai-Nien Mol Vis Research Article PURPOSE: MiRNAs are small noncoding RNAs that have been implicated in tumor development. They regulate target gene expression either by mRNA degradation or by translation repression. Activation of β-catenin has been linked to pterygium progression. Here, we hypothesize that β-catenin-associated miRNA, miRNA-221, and downstream p27Kip1 gene expression are correlated with the pathogenesis of pterygium. METHODS: We collected 120 pterygial and 120 normal conjunctival samples for this study. Immunohistochemistry and real-time reverse transcription (RT)–PCR were performed to determine β-catenin protein localization, miR-221, and p27Kip1 gene expression. Pterygium cell line (PECs) cell models were used to confirm the effect of β-catenin, miR-221, and p27Kip1 gene in the proliferation of pterygium cells. RESULTS: Seventy-two (60.0%) pterygial specimens showed high miR-221 expression levels, which was significantly higher than the control groups (13 of 120, 10.8%, p<0.0001). MiR-221 expression was significantly higher in β-catenin-nuclear/cytoplasmic-positive groups than in β-catenin membrane-positive and negative groups (p=0.001). We also found that p27Kip1 gene expression in pterygium was negatively correlated with miR-221 expression (p=0.002). In the clinical association, miR-221 expression was significantly higher in the fleshy and intermediate groups than in the atrophic group (p=0.007). The association of miR-221, p27Kip1 and proliferation of pterygium were also confirmed in the PECs model. CONCLUSIONS: Our study demonstrated that activation of β-catenin in pterygium may interact with miR-221, resulting in p27Kip1 gene downregulation that influences pterygium pathogenesis. Molecular Vision 2014-07-21 /pmc/articles/PMC4105113/ /pubmed/25053875 Text en Copyright © 2014 Molecular Vision. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed.
spellingShingle Research Article
Wu, Chueh-Wei
Cheng, Ya-Wen
Hsu, Nan-Yung
Yeh, Ken-Tu
Tsai, Yi-Yu
Chiang, Chun-Chi
Wang, Wei-Ran
Tung, Jai-Nien
MiRNA-221 negatively regulated downstream p27Kip1 gene expression involvement in pterygium pathogenesis
title MiRNA-221 negatively regulated downstream p27Kip1 gene expression involvement in pterygium pathogenesis
title_full MiRNA-221 negatively regulated downstream p27Kip1 gene expression involvement in pterygium pathogenesis
title_fullStr MiRNA-221 negatively regulated downstream p27Kip1 gene expression involvement in pterygium pathogenesis
title_full_unstemmed MiRNA-221 negatively regulated downstream p27Kip1 gene expression involvement in pterygium pathogenesis
title_short MiRNA-221 negatively regulated downstream p27Kip1 gene expression involvement in pterygium pathogenesis
title_sort mirna-221 negatively regulated downstream p27kip1 gene expression involvement in pterygium pathogenesis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105113/
https://www.ncbi.nlm.nih.gov/pubmed/25053875
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