Influence of Cofactor Regeneration Strategies on Preparative-Scale, Asymmetric Carbonyl Reductions by Engineered Escherichia coli

[Image: see text] This study was designed to determine whether whole cells or crude enzyme extracts are more effective for preparative-scale ketone reductions by dehydrogenases as well as learning which cofactor regeneration scheme is most effective. Based on results from three representative ketone...

Descripción completa

Detalles Bibliográficos
Autores principales: Dascier, Dimitri, Kambourakis, Spiros, Hua, Ling, Rozzell, J. David, Stewart, Jon D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105172/
https://www.ncbi.nlm.nih.gov/pubmed/25067899
http://dx.doi.org/10.1021/op400312n
_version_ 1782327330036776960
author Dascier, Dimitri
Kambourakis, Spiros
Hua, Ling
Rozzell, J. David
Stewart, Jon D.
author_facet Dascier, Dimitri
Kambourakis, Spiros
Hua, Ling
Rozzell, J. David
Stewart, Jon D.
author_sort Dascier, Dimitri
collection PubMed
description [Image: see text] This study was designed to determine whether whole cells or crude enzyme extracts are more effective for preparative-scale ketone reductions by dehydrogenases as well as learning which cofactor regeneration scheme is most effective. Based on results from three representative ketone substrates (an α-fluoro-β-keto ester, a bis-trifluoromethylated acetophenone, and a symmetrical β-diketone), our results demonstrate that several nicotinamide cofactor regeneration strategies can be applied to preparative-scale dehydrogenase-catalyzed reactions successfully.
format Online
Article
Text
id pubmed-4105172
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-41051722015-02-17 Influence of Cofactor Regeneration Strategies on Preparative-Scale, Asymmetric Carbonyl Reductions by Engineered Escherichia coli Dascier, Dimitri Kambourakis, Spiros Hua, Ling Rozzell, J. David Stewart, Jon D. Org Process Res Dev [Image: see text] This study was designed to determine whether whole cells or crude enzyme extracts are more effective for preparative-scale ketone reductions by dehydrogenases as well as learning which cofactor regeneration scheme is most effective. Based on results from three representative ketone substrates (an α-fluoro-β-keto ester, a bis-trifluoromethylated acetophenone, and a symmetrical β-diketone), our results demonstrate that several nicotinamide cofactor regeneration strategies can be applied to preparative-scale dehydrogenase-catalyzed reactions successfully. American Chemical Society 2014-02-17 2014-06-20 /pmc/articles/PMC4105172/ /pubmed/25067899 http://dx.doi.org/10.1021/op400312n Text en Copyright © 2014 American Chemical Society Terms of Use (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html)
spellingShingle Dascier, Dimitri
Kambourakis, Spiros
Hua, Ling
Rozzell, J. David
Stewart, Jon D.
Influence of Cofactor Regeneration Strategies on Preparative-Scale, Asymmetric Carbonyl Reductions by Engineered Escherichia coli
title Influence of Cofactor Regeneration Strategies on Preparative-Scale, Asymmetric Carbonyl Reductions by Engineered Escherichia coli
title_full Influence of Cofactor Regeneration Strategies on Preparative-Scale, Asymmetric Carbonyl Reductions by Engineered Escherichia coli
title_fullStr Influence of Cofactor Regeneration Strategies on Preparative-Scale, Asymmetric Carbonyl Reductions by Engineered Escherichia coli
title_full_unstemmed Influence of Cofactor Regeneration Strategies on Preparative-Scale, Asymmetric Carbonyl Reductions by Engineered Escherichia coli
title_short Influence of Cofactor Regeneration Strategies on Preparative-Scale, Asymmetric Carbonyl Reductions by Engineered Escherichia coli
title_sort influence of cofactor regeneration strategies on preparative-scale, asymmetric carbonyl reductions by engineered escherichia coli
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105172/
https://www.ncbi.nlm.nih.gov/pubmed/25067899
http://dx.doi.org/10.1021/op400312n
work_keys_str_mv AT dascierdimitri influenceofcofactorregenerationstrategiesonpreparativescaleasymmetriccarbonylreductionsbyengineeredescherichiacoli
AT kambourakisspiros influenceofcofactorregenerationstrategiesonpreparativescaleasymmetriccarbonylreductionsbyengineeredescherichiacoli
AT hualing influenceofcofactorregenerationstrategiesonpreparativescaleasymmetriccarbonylreductionsbyengineeredescherichiacoli
AT rozzelljdavid influenceofcofactorregenerationstrategiesonpreparativescaleasymmetriccarbonylreductionsbyengineeredescherichiacoli
AT stewartjond influenceofcofactorregenerationstrategiesonpreparativescaleasymmetriccarbonylreductionsbyengineeredescherichiacoli

Ejemplares similares