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Microsatellite markers in Rhodiola (Crassulaceae), a medicinal herb genus widely used in traditional Chinese medicine(1)

• Premise of the study: Microsatellite loci are described for Rhodiola, a medicinal herb genus widely used in traditional Chinese medicine. • Methods and Results: A total of 17 polymorphic microsatellite primer pairs were developed using the combined biotin capture method. The number of alleles per...

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Detalles Bibliográficos
Autores principales: You, Jianling, Liu, Wensheng, Zhao, Yao, Zhu, Yongqing, Zhang, Wenju, Wang, Yuguo, Lu, Fan, Song, Zhiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105280/
https://www.ncbi.nlm.nih.gov/pubmed/25202523
http://dx.doi.org/10.3732/apps.1200219
Descripción
Sumario:• Premise of the study: Microsatellite loci are described for Rhodiola, a medicinal herb genus widely used in traditional Chinese medicine. • Methods and Results: A total of 17 polymorphic microsatellite primer pairs were developed using the combined biotin capture method. The number of alleles per locus ranged from one to 12 across 192 individuals from R. bupleuroides, R. crenulata, R. fastigiata, and R. sacra, and the mean observed and expected heterozygosities ranged from 0.177 to 0.412 and from 0.363 to 0.578, respectively. • Conclusions: The results demonstrate the potential use of this new set of microsatellite markers for genotyping individuals and estimating genetic diversity in Rhodiola.