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Characterization of microsatellite loci in Tilia platyphyllos (Malvaceae) and cross-amplification in related species(1)
• Premise of the study: Microsatellite markers in the genus Tilia were developed to investigate the genetic variation in T. platyphyllos and its relationship with T. cordata. • Methods and Results: Fifteen microsatellite markers were developed using a microsatellite enrichment protocol. Most loci sh...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105290/ https://www.ncbi.nlm.nih.gov/pubmed/25202533 http://dx.doi.org/10.3732/apps.1200386 |
Sumario: | • Premise of the study: Microsatellite markers in the genus Tilia were developed to investigate the genetic variation in T. platyphyllos and its relationship with T. cordata. • Methods and Results: Fifteen microsatellite markers were developed using a microsatellite enrichment protocol. Most loci show a high level of polymorphism in two T. platyphyllos populations from France. The number of alleles ranged from one to 15, with a mean of 8.96. The mean observed and expected heterozygosities were 0.71 and 0.70, respectively. Cross-amplification results indicated that 12 out of 15 loci amplified polymorphic loci in 23 species in the genus. • Conclusions: These markers will be useful tools for investigating the phylogeography and hybridization of Tilia species. |
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