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Isolation and characterization of microsatellite markers for Canavalia cathartica and C. lineata (Fabaceae)(1)

• Premise of the study: Microsatellite markers were developed for the sea-dispersed legume Canavalia cathartica to investigate its genetic diversity and population structure. We also applied these microsatellite markers to the closely related species C. lineata. • Methods and Results: Ten primer set...

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Detalles Bibliográficos
Autores principales: Yamashiro, Asuka, Yamashiro, Tadashi, Tateishi, Yoichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105347/
https://www.ncbi.nlm.nih.gov/pubmed/25202474
http://dx.doi.org/10.3732/apps.1200111
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author Yamashiro, Asuka
Yamashiro, Tadashi
Tateishi, Yoichi
author_facet Yamashiro, Asuka
Yamashiro, Tadashi
Tateishi, Yoichi
author_sort Yamashiro, Asuka
collection PubMed
description • Premise of the study: Microsatellite markers were developed for the sea-dispersed legume Canavalia cathartica to investigate its genetic diversity and population structure. We also applied these microsatellite markers to the closely related species C. lineata. • Methods and Results: Ten primer sets were developed for C. cathartica, and all of the primers were amplified in C. lineata. The number of alleles per locus ranged from two to 13 and one to 10 for C. cathartica and C. lineata, respectively. The expected heterozygosity ranged from 0.375 to 0.870 and from 0.071 to 0.877 for C. cathartica and C. lineata, respectively. • Conclusions: The 10 microsatellite markers developed in this study can be used to analyze the population genetic structure of C. cathartica and other related taxa.
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spelling pubmed-41053472014-09-08 Isolation and characterization of microsatellite markers for Canavalia cathartica and C. lineata (Fabaceae)(1) Yamashiro, Asuka Yamashiro, Tadashi Tateishi, Yoichi Appl Plant Sci Primer Note • Premise of the study: Microsatellite markers were developed for the sea-dispersed legume Canavalia cathartica to investigate its genetic diversity and population structure. We also applied these microsatellite markers to the closely related species C. lineata. • Methods and Results: Ten primer sets were developed for C. cathartica, and all of the primers were amplified in C. lineata. The number of alleles per locus ranged from two to 13 and one to 10 for C. cathartica and C. lineata, respectively. The expected heterozygosity ranged from 0.375 to 0.870 and from 0.071 to 0.877 for C. cathartica and C. lineata, respectively. • Conclusions: The 10 microsatellite markers developed in this study can be used to analyze the population genetic structure of C. cathartica and other related taxa. Botanical Society of America 2013-01-02 /pmc/articles/PMC4105347/ /pubmed/25202474 http://dx.doi.org/10.3732/apps.1200111 Text en © 2013 Botanical Society of America
spellingShingle Primer Note
Yamashiro, Asuka
Yamashiro, Tadashi
Tateishi, Yoichi
Isolation and characterization of microsatellite markers for Canavalia cathartica and C. lineata (Fabaceae)(1)
title Isolation and characterization of microsatellite markers for Canavalia cathartica and C. lineata (Fabaceae)(1)
title_full Isolation and characterization of microsatellite markers for Canavalia cathartica and C. lineata (Fabaceae)(1)
title_fullStr Isolation and characterization of microsatellite markers for Canavalia cathartica and C. lineata (Fabaceae)(1)
title_full_unstemmed Isolation and characterization of microsatellite markers for Canavalia cathartica and C. lineata (Fabaceae)(1)
title_short Isolation and characterization of microsatellite markers for Canavalia cathartica and C. lineata (Fabaceae)(1)
title_sort isolation and characterization of microsatellite markers for canavalia cathartica and c. lineata (fabaceae)(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105347/
https://www.ncbi.nlm.nih.gov/pubmed/25202474
http://dx.doi.org/10.3732/apps.1200111
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