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Enhancing PCR amplification of DNA from recalcitrant plant specimens using a trehalose-based additive(1)

• Premise of the study: PCR amplification of DNA extracted from plants is sometimes difficult due to the presence of inhibitory compounds. An effective method to overcome the inhibitory effect of compounds that contaminate DNA from difficult plant specimens is needed. • Methods and Results: The effe...

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Detalles Bibliográficos
Autores principales: Samarakoon, Tharangamala, Wang, Shiao Y., Alford, Mac H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105354/
https://www.ncbi.nlm.nih.gov/pubmed/25202481
http://dx.doi.org/10.3732/apps.1200236
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author Samarakoon, Tharangamala
Wang, Shiao Y.
Alford, Mac H.
author_facet Samarakoon, Tharangamala
Wang, Shiao Y.
Alford, Mac H.
author_sort Samarakoon, Tharangamala
collection PubMed
description • Premise of the study: PCR amplification of DNA extracted from plants is sometimes difficult due to the presence of inhibitory compounds. An effective method to overcome the inhibitory effect of compounds that contaminate DNA from difficult plant specimens is needed. • Methods and Results: The effectiveness of a PCR additive reagent containing trehalose, bovine serum albumin (BSA), and polysorbate-20 (Tween-20) (TBT-PAR) was tested. PCR of DNA extracted from fresh, silica-dried, and herbarium leaf material of species of Achariaceae, Asteraceae, Lacistemataceae, and Samydaceae that failed using standard techniques were successful with the addition of TBT-PAR. • Conclusions: The addition of TBT-PAR during routine PCR is an effective method to improve amplification of DNA extracted from herbarium specimens or plants that are known to contain PCR inhibitors.
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spelling pubmed-41053542014-09-08 Enhancing PCR amplification of DNA from recalcitrant plant specimens using a trehalose-based additive(1) Samarakoon, Tharangamala Wang, Shiao Y. Alford, Mac H. Appl Plant Sci Protocol Note • Premise of the study: PCR amplification of DNA extracted from plants is sometimes difficult due to the presence of inhibitory compounds. An effective method to overcome the inhibitory effect of compounds that contaminate DNA from difficult plant specimens is needed. • Methods and Results: The effectiveness of a PCR additive reagent containing trehalose, bovine serum albumin (BSA), and polysorbate-20 (Tween-20) (TBT-PAR) was tested. PCR of DNA extracted from fresh, silica-dried, and herbarium leaf material of species of Achariaceae, Asteraceae, Lacistemataceae, and Samydaceae that failed using standard techniques were successful with the addition of TBT-PAR. • Conclusions: The addition of TBT-PAR during routine PCR is an effective method to improve amplification of DNA extracted from herbarium specimens or plants that are known to contain PCR inhibitors. Botanical Society of America 2013-01-02 /pmc/articles/PMC4105354/ /pubmed/25202481 http://dx.doi.org/10.3732/apps.1200236 Text en © 2013 Botanical Society of America
spellingShingle Protocol Note
Samarakoon, Tharangamala
Wang, Shiao Y.
Alford, Mac H.
Enhancing PCR amplification of DNA from recalcitrant plant specimens using a trehalose-based additive(1)
title Enhancing PCR amplification of DNA from recalcitrant plant specimens using a trehalose-based additive(1)
title_full Enhancing PCR amplification of DNA from recalcitrant plant specimens using a trehalose-based additive(1)
title_fullStr Enhancing PCR amplification of DNA from recalcitrant plant specimens using a trehalose-based additive(1)
title_full_unstemmed Enhancing PCR amplification of DNA from recalcitrant plant specimens using a trehalose-based additive(1)
title_short Enhancing PCR amplification of DNA from recalcitrant plant specimens using a trehalose-based additive(1)
title_sort enhancing pcr amplification of dna from recalcitrant plant specimens using a trehalose-based additive(1)
topic Protocol Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105354/
https://www.ncbi.nlm.nih.gov/pubmed/25202481
http://dx.doi.org/10.3732/apps.1200236
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