Cargando…
Enhancing PCR amplification of DNA from recalcitrant plant specimens using a trehalose-based additive(1)
• Premise of the study: PCR amplification of DNA extracted from plants is sometimes difficult due to the presence of inhibitory compounds. An effective method to overcome the inhibitory effect of compounds that contaminate DNA from difficult plant specimens is needed. • Methods and Results: The effe...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105354/ https://www.ncbi.nlm.nih.gov/pubmed/25202481 http://dx.doi.org/10.3732/apps.1200236 |
_version_ | 1782327349611593728 |
---|---|
author | Samarakoon, Tharangamala Wang, Shiao Y. Alford, Mac H. |
author_facet | Samarakoon, Tharangamala Wang, Shiao Y. Alford, Mac H. |
author_sort | Samarakoon, Tharangamala |
collection | PubMed |
description | • Premise of the study: PCR amplification of DNA extracted from plants is sometimes difficult due to the presence of inhibitory compounds. An effective method to overcome the inhibitory effect of compounds that contaminate DNA from difficult plant specimens is needed. • Methods and Results: The effectiveness of a PCR additive reagent containing trehalose, bovine serum albumin (BSA), and polysorbate-20 (Tween-20) (TBT-PAR) was tested. PCR of DNA extracted from fresh, silica-dried, and herbarium leaf material of species of Achariaceae, Asteraceae, Lacistemataceae, and Samydaceae that failed using standard techniques were successful with the addition of TBT-PAR. • Conclusions: The addition of TBT-PAR during routine PCR is an effective method to improve amplification of DNA extracted from herbarium specimens or plants that are known to contain PCR inhibitors. |
format | Online Article Text |
id | pubmed-4105354 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-41053542014-09-08 Enhancing PCR amplification of DNA from recalcitrant plant specimens using a trehalose-based additive(1) Samarakoon, Tharangamala Wang, Shiao Y. Alford, Mac H. Appl Plant Sci Protocol Note • Premise of the study: PCR amplification of DNA extracted from plants is sometimes difficult due to the presence of inhibitory compounds. An effective method to overcome the inhibitory effect of compounds that contaminate DNA from difficult plant specimens is needed. • Methods and Results: The effectiveness of a PCR additive reagent containing trehalose, bovine serum albumin (BSA), and polysorbate-20 (Tween-20) (TBT-PAR) was tested. PCR of DNA extracted from fresh, silica-dried, and herbarium leaf material of species of Achariaceae, Asteraceae, Lacistemataceae, and Samydaceae that failed using standard techniques were successful with the addition of TBT-PAR. • Conclusions: The addition of TBT-PAR during routine PCR is an effective method to improve amplification of DNA extracted from herbarium specimens or plants that are known to contain PCR inhibitors. Botanical Society of America 2013-01-02 /pmc/articles/PMC4105354/ /pubmed/25202481 http://dx.doi.org/10.3732/apps.1200236 Text en © 2013 Botanical Society of America |
spellingShingle | Protocol Note Samarakoon, Tharangamala Wang, Shiao Y. Alford, Mac H. Enhancing PCR amplification of DNA from recalcitrant plant specimens using a trehalose-based additive(1) |
title | Enhancing PCR amplification of DNA from recalcitrant plant specimens using a trehalose-based additive(1) |
title_full | Enhancing PCR amplification of DNA from recalcitrant plant specimens using a trehalose-based additive(1) |
title_fullStr | Enhancing PCR amplification of DNA from recalcitrant plant specimens using a trehalose-based additive(1) |
title_full_unstemmed | Enhancing PCR amplification of DNA from recalcitrant plant specimens using a trehalose-based additive(1) |
title_short | Enhancing PCR amplification of DNA from recalcitrant plant specimens using a trehalose-based additive(1) |
title_sort | enhancing pcr amplification of dna from recalcitrant plant specimens using a trehalose-based additive(1) |
topic | Protocol Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105354/ https://www.ncbi.nlm.nih.gov/pubmed/25202481 http://dx.doi.org/10.3732/apps.1200236 |
work_keys_str_mv | AT samarakoontharangamala enhancingpcramplificationofdnafromrecalcitrantplantspecimensusingatrehalosebasedadditive1 AT wangshiaoy enhancingpcramplificationofdnafromrecalcitrantplantspecimensusingatrehalosebasedadditive1 AT alfordmach enhancingpcramplificationofdnafromrecalcitrantplantspecimensusingatrehalosebasedadditive1 |