Cargando…
First identification of polymorphic microsatellite markers in the Burgundy truffle, Tuber aestivum (Tuberaceae)(1)
• Premise of the study: Tuber aestivum, the most common truffle in Europe, plays an important role in the commercial truffle market. For the first time, microsatellite primers were developed to investigate polymorphism within this species. • Methods and Results: Using direct shotgun pyrosequencing,...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105367/ https://www.ncbi.nlm.nih.gov/pubmed/25202513 http://dx.doi.org/10.3732/apps.1200220 |
_version_ | 1782327351065968640 |
---|---|
author | Molinier, Virginie Murat, Claude Morin, Emmanuelle Gollotte, Armelle Wipf, Daniel Martin, Francis |
author_facet | Molinier, Virginie Murat, Claude Morin, Emmanuelle Gollotte, Armelle Wipf, Daniel Martin, Francis |
author_sort | Molinier, Virginie |
collection | PubMed |
description | • Premise of the study: Tuber aestivum, the most common truffle in Europe, plays an important role in the commercial truffle market. For the first time, microsatellite primers were developed to investigate polymorphism within this species. • Methods and Results: Using direct shotgun pyrosequencing, 15 polymorphic microsatellites were identified out of the 7784 perfect microsatellites present in the 534620 reads obtained. Tested on 75 samples, these microsatellites were highly polymorphic. The number of alleles varied from four to 15, and the expected heterozygosity ranged from 0.266 to 0.620. A multilocus analysis allowed the identification of 63 genotypes over the 75 samples analyzed. • Conclusions: Direct shotgun pyrosequencing is a fast and relatively low-cost technique allowing identification of microsatellites in nonmodel species. The microsatellites developed in this study will be useful in population genetic studies to infer the evolutionary history of this species. |
format | Online Article Text |
id | pubmed-4105367 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-41053672014-09-08 First identification of polymorphic microsatellite markers in the Burgundy truffle, Tuber aestivum (Tuberaceae)(1) Molinier, Virginie Murat, Claude Morin, Emmanuelle Gollotte, Armelle Wipf, Daniel Martin, Francis Appl Plant Sci Primer Note • Premise of the study: Tuber aestivum, the most common truffle in Europe, plays an important role in the commercial truffle market. For the first time, microsatellite primers were developed to investigate polymorphism within this species. • Methods and Results: Using direct shotgun pyrosequencing, 15 polymorphic microsatellites were identified out of the 7784 perfect microsatellites present in the 534620 reads obtained. Tested on 75 samples, these microsatellites were highly polymorphic. The number of alleles varied from four to 15, and the expected heterozygosity ranged from 0.266 to 0.620. A multilocus analysis allowed the identification of 63 genotypes over the 75 samples analyzed. • Conclusions: Direct shotgun pyrosequencing is a fast and relatively low-cost technique allowing identification of microsatellites in nonmodel species. The microsatellites developed in this study will be useful in population genetic studies to infer the evolutionary history of this species. Botanical Society of America 2013-01-31 /pmc/articles/PMC4105367/ /pubmed/25202513 http://dx.doi.org/10.3732/apps.1200220 Text en © 2013 Botanical Society of America |
spellingShingle | Primer Note Molinier, Virginie Murat, Claude Morin, Emmanuelle Gollotte, Armelle Wipf, Daniel Martin, Francis First identification of polymorphic microsatellite markers in the Burgundy truffle, Tuber aestivum (Tuberaceae)(1) |
title | First identification of polymorphic microsatellite markers in the Burgundy truffle, Tuber aestivum (Tuberaceae)(1) |
title_full | First identification of polymorphic microsatellite markers in the Burgundy truffle, Tuber aestivum (Tuberaceae)(1) |
title_fullStr | First identification of polymorphic microsatellite markers in the Burgundy truffle, Tuber aestivum (Tuberaceae)(1) |
title_full_unstemmed | First identification of polymorphic microsatellite markers in the Burgundy truffle, Tuber aestivum (Tuberaceae)(1) |
title_short | First identification of polymorphic microsatellite markers in the Burgundy truffle, Tuber aestivum (Tuberaceae)(1) |
title_sort | first identification of polymorphic microsatellite markers in the burgundy truffle, tuber aestivum (tuberaceae)(1) |
topic | Primer Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105367/ https://www.ncbi.nlm.nih.gov/pubmed/25202513 http://dx.doi.org/10.3732/apps.1200220 |
work_keys_str_mv | AT moliniervirginie firstidentificationofpolymorphicmicrosatellitemarkersintheburgundytruffletuberaestivumtuberaceae1 AT muratclaude firstidentificationofpolymorphicmicrosatellitemarkersintheburgundytruffletuberaestivumtuberaceae1 AT morinemmanuelle firstidentificationofpolymorphicmicrosatellitemarkersintheburgundytruffletuberaestivumtuberaceae1 AT gollottearmelle firstidentificationofpolymorphicmicrosatellitemarkersintheburgundytruffletuberaestivumtuberaceae1 AT wipfdaniel firstidentificationofpolymorphicmicrosatellitemarkersintheburgundytruffletuberaestivumtuberaceae1 AT martinfrancis firstidentificationofpolymorphicmicrosatellitemarkersintheburgundytruffletuberaestivumtuberaceae1 |