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Development of microsatellite markers in the hexaploid aquatic macrophyte, Myriophyllum spicatum (Haloragaceae)(1)

• Premise of the study: We developed microsatellite primers to investigate genetic diversity and population genetic structure of the cosmopolitan submerged plant Myriophyllum spicatum. • Methods and Results: Twenty microsatellite loci were identified in M. spicatum using the microsatellite-enriched...

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Detalles Bibliográficos
Autores principales: Wu, Zhi-Gang, Yu, Dan, Xu, Xin-Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105368/
https://www.ncbi.nlm.nih.gov/pubmed/25202514
http://dx.doi.org/10.3732/apps.1200230
Descripción
Sumario:• Premise of the study: We developed microsatellite primers to investigate genetic diversity and population genetic structure of the cosmopolitan submerged plant Myriophyllum spicatum. • Methods and Results: Twenty microsatellite loci were identified in M. spicatum using the microsatellite-enriched library method. The numbers of alleles per locus ranged from one to 13, and the expected heterozygosity varied from 0 to 0.873 with a mean of 0.504 in two Chinese populations of M. spicatum. All of the loci were also found to be amplifiable in the related species M. verticillatum and M. sibiricum. • Conclusions: The results indicate that these markers will be significant for studies of population genetic structure and evolutionary history of M. spicatum as well as some of its related species.