Cargando…

Characterization of microsatellite loci in the lichen fungus Lobaria pulmonaria (Lobariaceae)(1)

• Premise of the study: Microsatellite loci were developed for the threatened haploid lichen fungus Lobaria pulmonaria to increase the resolution to identify clonal individuals, and to study its population subdivision. • Methods and Results: We developed 14 microsatellite markers from 454 DNA sequen...

Descripción completa

Detalles Bibliográficos
Autores principales: Werth, Silke, Cornejo, Carolina, Scheidegger, Christoph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105370/
https://www.ncbi.nlm.nih.gov/pubmed/25202516
http://dx.doi.org/10.3732/apps.1200290
_version_ 1782327351804166144
author Werth, Silke
Cornejo, Carolina
Scheidegger, Christoph
author_facet Werth, Silke
Cornejo, Carolina
Scheidegger, Christoph
author_sort Werth, Silke
collection PubMed
description • Premise of the study: Microsatellite loci were developed for the threatened haploid lichen fungus Lobaria pulmonaria to increase the resolution to identify clonal individuals, and to study its population subdivision. • Methods and Results: We developed 14 microsatellite markers from 454 DNA sequencing data of L. pulmonaria and tested for cross-amplification with L. immixta and L. macaronesica. The number of alleles per locus ranged from two to 23. Nei’s unbiased gene diversity, averaged over loci, ranged from 0.434 to 0.517 in the three studied populations. • Conclusions: The new markers will increase the genetic resolution in studies that aim at disentangling clones in L. pulmonaria and may be useful for closely related species within Lobaria sect. Lobaria.
format Online
Article
Text
id pubmed-4105370
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Botanical Society of America
record_format MEDLINE/PubMed
spelling pubmed-41053702014-09-08 Characterization of microsatellite loci in the lichen fungus Lobaria pulmonaria (Lobariaceae)(1) Werth, Silke Cornejo, Carolina Scheidegger, Christoph Appl Plant Sci Primer Note • Premise of the study: Microsatellite loci were developed for the threatened haploid lichen fungus Lobaria pulmonaria to increase the resolution to identify clonal individuals, and to study its population subdivision. • Methods and Results: We developed 14 microsatellite markers from 454 DNA sequencing data of L. pulmonaria and tested for cross-amplification with L. immixta and L. macaronesica. The number of alleles per locus ranged from two to 23. Nei’s unbiased gene diversity, averaged over loci, ranged from 0.434 to 0.517 in the three studied populations. • Conclusions: The new markers will increase the genetic resolution in studies that aim at disentangling clones in L. pulmonaria and may be useful for closely related species within Lobaria sect. Lobaria. Botanical Society of America 2013-01-31 /pmc/articles/PMC4105370/ /pubmed/25202516 http://dx.doi.org/10.3732/apps.1200290 Text en © 2013 Botanical Society of America
spellingShingle Primer Note
Werth, Silke
Cornejo, Carolina
Scheidegger, Christoph
Characterization of microsatellite loci in the lichen fungus Lobaria pulmonaria (Lobariaceae)(1)
title Characterization of microsatellite loci in the lichen fungus Lobaria pulmonaria (Lobariaceae)(1)
title_full Characterization of microsatellite loci in the lichen fungus Lobaria pulmonaria (Lobariaceae)(1)
title_fullStr Characterization of microsatellite loci in the lichen fungus Lobaria pulmonaria (Lobariaceae)(1)
title_full_unstemmed Characterization of microsatellite loci in the lichen fungus Lobaria pulmonaria (Lobariaceae)(1)
title_short Characterization of microsatellite loci in the lichen fungus Lobaria pulmonaria (Lobariaceae)(1)
title_sort characterization of microsatellite loci in the lichen fungus lobaria pulmonaria (lobariaceae)(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105370/
https://www.ncbi.nlm.nih.gov/pubmed/25202516
http://dx.doi.org/10.3732/apps.1200290
work_keys_str_mv AT werthsilke characterizationofmicrosatellitelociinthelichenfunguslobariapulmonarialobariaceae1
AT cornejocarolina characterizationofmicrosatellitelociinthelichenfunguslobariapulmonarialobariaceae1
AT scheideggerchristoph characterizationofmicrosatellitelociinthelichenfunguslobariapulmonarialobariaceae1