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Characterization of microsatellite loci in the lichen fungus Lobaria pulmonaria (Lobariaceae)(1)
• Premise of the study: Microsatellite loci were developed for the threatened haploid lichen fungus Lobaria pulmonaria to increase the resolution to identify clonal individuals, and to study its population subdivision. • Methods and Results: We developed 14 microsatellite markers from 454 DNA sequen...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105370/ https://www.ncbi.nlm.nih.gov/pubmed/25202516 http://dx.doi.org/10.3732/apps.1200290 |
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author | Werth, Silke Cornejo, Carolina Scheidegger, Christoph |
author_facet | Werth, Silke Cornejo, Carolina Scheidegger, Christoph |
author_sort | Werth, Silke |
collection | PubMed |
description | • Premise of the study: Microsatellite loci were developed for the threatened haploid lichen fungus Lobaria pulmonaria to increase the resolution to identify clonal individuals, and to study its population subdivision. • Methods and Results: We developed 14 microsatellite markers from 454 DNA sequencing data of L. pulmonaria and tested for cross-amplification with L. immixta and L. macaronesica. The number of alleles per locus ranged from two to 23. Nei’s unbiased gene diversity, averaged over loci, ranged from 0.434 to 0.517 in the three studied populations. • Conclusions: The new markers will increase the genetic resolution in studies that aim at disentangling clones in L. pulmonaria and may be useful for closely related species within Lobaria sect. Lobaria. |
format | Online Article Text |
id | pubmed-4105370 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-41053702014-09-08 Characterization of microsatellite loci in the lichen fungus Lobaria pulmonaria (Lobariaceae)(1) Werth, Silke Cornejo, Carolina Scheidegger, Christoph Appl Plant Sci Primer Note • Premise of the study: Microsatellite loci were developed for the threatened haploid lichen fungus Lobaria pulmonaria to increase the resolution to identify clonal individuals, and to study its population subdivision. • Methods and Results: We developed 14 microsatellite markers from 454 DNA sequencing data of L. pulmonaria and tested for cross-amplification with L. immixta and L. macaronesica. The number of alleles per locus ranged from two to 23. Nei’s unbiased gene diversity, averaged over loci, ranged from 0.434 to 0.517 in the three studied populations. • Conclusions: The new markers will increase the genetic resolution in studies that aim at disentangling clones in L. pulmonaria and may be useful for closely related species within Lobaria sect. Lobaria. Botanical Society of America 2013-01-31 /pmc/articles/PMC4105370/ /pubmed/25202516 http://dx.doi.org/10.3732/apps.1200290 Text en © 2013 Botanical Society of America |
spellingShingle | Primer Note Werth, Silke Cornejo, Carolina Scheidegger, Christoph Characterization of microsatellite loci in the lichen fungus Lobaria pulmonaria (Lobariaceae)(1) |
title | Characterization of microsatellite loci in the lichen fungus Lobaria pulmonaria (Lobariaceae)(1) |
title_full | Characterization of microsatellite loci in the lichen fungus Lobaria pulmonaria (Lobariaceae)(1) |
title_fullStr | Characterization of microsatellite loci in the lichen fungus Lobaria pulmonaria (Lobariaceae)(1) |
title_full_unstemmed | Characterization of microsatellite loci in the lichen fungus Lobaria pulmonaria (Lobariaceae)(1) |
title_short | Characterization of microsatellite loci in the lichen fungus Lobaria pulmonaria (Lobariaceae)(1) |
title_sort | characterization of microsatellite loci in the lichen fungus lobaria pulmonaria (lobariaceae)(1) |
topic | Primer Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105370/ https://www.ncbi.nlm.nih.gov/pubmed/25202516 http://dx.doi.org/10.3732/apps.1200290 |
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