Cargando…
Comparison between Culture and a Multiplex Quantitative Real-Time Polymerase Chain Reaction Assay Detecting Ureaplasma urealyticum and U. parvum
A novel multiplex quantitative real-time polymerase chain reaction (qPCR) for simultaneous detection of U. urealyticum and U. parvum was developed and compared with quantitative culture in Shepard's 10 C medium for ureaplasmas in urethral swabs from 129 men and 66 women, and cervical swabs from...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105565/ https://www.ncbi.nlm.nih.gov/pubmed/25047036 http://dx.doi.org/10.1371/journal.pone.0102743 |
_version_ | 1782327391655297024 |
---|---|
author | Frølund, Maria Björnelius, Eva Lidbrink, Peter Ahrens, Peter Jensen, Jørgen Skov |
author_facet | Frølund, Maria Björnelius, Eva Lidbrink, Peter Ahrens, Peter Jensen, Jørgen Skov |
author_sort | Frølund, Maria |
collection | PubMed |
description | A novel multiplex quantitative real-time polymerase chain reaction (qPCR) for simultaneous detection of U. urealyticum and U. parvum was developed and compared with quantitative culture in Shepard's 10 C medium for ureaplasmas in urethral swabs from 129 men and 66 women, and cervical swabs from 61 women. Using culture as the gold standard, the sensitivity of the qPCR was 96% and 95% for female urethral and cervical swabs, respectively. In male urethral swabs the sensitivity was 89%. The corresponding specificities were 100%, 87% and 99%. The qPCR showed a linear increasing DNA copy number with increasing colour-changing units. Although slightly less sensitive than culture, this multiplex qPCR assay detecting U. urealyticum and U. parvum constitutes a simple and fast alternative to the traditional methods for identification of ureaplasmas and allows simultaneous species differentiation and quantitation in clinical samples. Furthermore, specimens overgrown by other bacteria using the culture method can be evaluated in the qPCR. |
format | Online Article Text |
id | pubmed-4105565 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41055652014-07-23 Comparison between Culture and a Multiplex Quantitative Real-Time Polymerase Chain Reaction Assay Detecting Ureaplasma urealyticum and U. parvum Frølund, Maria Björnelius, Eva Lidbrink, Peter Ahrens, Peter Jensen, Jørgen Skov PLoS One Research Article A novel multiplex quantitative real-time polymerase chain reaction (qPCR) for simultaneous detection of U. urealyticum and U. parvum was developed and compared with quantitative culture in Shepard's 10 C medium for ureaplasmas in urethral swabs from 129 men and 66 women, and cervical swabs from 61 women. Using culture as the gold standard, the sensitivity of the qPCR was 96% and 95% for female urethral and cervical swabs, respectively. In male urethral swabs the sensitivity was 89%. The corresponding specificities were 100%, 87% and 99%. The qPCR showed a linear increasing DNA copy number with increasing colour-changing units. Although slightly less sensitive than culture, this multiplex qPCR assay detecting U. urealyticum and U. parvum constitutes a simple and fast alternative to the traditional methods for identification of ureaplasmas and allows simultaneous species differentiation and quantitation in clinical samples. Furthermore, specimens overgrown by other bacteria using the culture method can be evaluated in the qPCR. Public Library of Science 2014-07-21 /pmc/articles/PMC4105565/ /pubmed/25047036 http://dx.doi.org/10.1371/journal.pone.0102743 Text en © 2014 Frølund et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Frølund, Maria Björnelius, Eva Lidbrink, Peter Ahrens, Peter Jensen, Jørgen Skov Comparison between Culture and a Multiplex Quantitative Real-Time Polymerase Chain Reaction Assay Detecting Ureaplasma urealyticum and U. parvum |
title | Comparison between Culture and a Multiplex Quantitative Real-Time Polymerase Chain Reaction Assay Detecting Ureaplasma urealyticum and U. parvum
|
title_full | Comparison between Culture and a Multiplex Quantitative Real-Time Polymerase Chain Reaction Assay Detecting Ureaplasma urealyticum and U. parvum
|
title_fullStr | Comparison between Culture and a Multiplex Quantitative Real-Time Polymerase Chain Reaction Assay Detecting Ureaplasma urealyticum and U. parvum
|
title_full_unstemmed | Comparison between Culture and a Multiplex Quantitative Real-Time Polymerase Chain Reaction Assay Detecting Ureaplasma urealyticum and U. parvum
|
title_short | Comparison between Culture and a Multiplex Quantitative Real-Time Polymerase Chain Reaction Assay Detecting Ureaplasma urealyticum and U. parvum
|
title_sort | comparison between culture and a multiplex quantitative real-time polymerase chain reaction assay detecting ureaplasma urealyticum and u. parvum |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105565/ https://www.ncbi.nlm.nih.gov/pubmed/25047036 http://dx.doi.org/10.1371/journal.pone.0102743 |
work_keys_str_mv | AT frølundmaria comparisonbetweencultureandamultiplexquantitativerealtimepolymerasechainreactionassaydetectingureaplasmaurealyticumanduparvum AT bjorneliuseva comparisonbetweencultureandamultiplexquantitativerealtimepolymerasechainreactionassaydetectingureaplasmaurealyticumanduparvum AT lidbrinkpeter comparisonbetweencultureandamultiplexquantitativerealtimepolymerasechainreactionassaydetectingureaplasmaurealyticumanduparvum AT ahrenspeter comparisonbetweencultureandamultiplexquantitativerealtimepolymerasechainreactionassaydetectingureaplasmaurealyticumanduparvum AT jensenjørgenskov comparisonbetweencultureandamultiplexquantitativerealtimepolymerasechainreactionassaydetectingureaplasmaurealyticumanduparvum |