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Bilateral uterine vessel ligation as a model of intrauterine growth restriction in mice

BACKGROUND: Intrauterine Growth Restriction (IUGR) occurs in up to 10% of pregnancies and is considered as a major risk to develop various diseases in adulthood, such as cardiovascular diseases, insulin resistance, hypertension or end stage kidney disease. Several IUGR models have been developed in...

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Autores principales: Janot, Mathilde, Cortes-Dubly, Marie-Laure, Rodriguez, Stéphane, Huynh-Do, Uyen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105874/
https://www.ncbi.nlm.nih.gov/pubmed/25004931
http://dx.doi.org/10.1186/1477-7827-12-62
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author Janot, Mathilde
Cortes-Dubly, Marie-Laure
Rodriguez, Stéphane
Huynh-Do, Uyen
author_facet Janot, Mathilde
Cortes-Dubly, Marie-Laure
Rodriguez, Stéphane
Huynh-Do, Uyen
author_sort Janot, Mathilde
collection PubMed
description BACKGROUND: Intrauterine Growth Restriction (IUGR) occurs in up to 10% of pregnancies and is considered as a major risk to develop various diseases in adulthood, such as cardiovascular diseases, insulin resistance, hypertension or end stage kidney disease. Several IUGR models have been developed in order to understand the biological processes linked to fetal growth retardation, most of them being rat or mouse models and nutritional models. In order to reproduce altered placental flow, surgical models have also been developed, and among them bilateral uterine ligation has been frequently used. Nevertheless, this model has never been developed in the mouse, although murine tools display multiple advantages for biological research. The aim of this work was therefore to develop a mouse model of bilateral uterine ligation as a surgical model of IUGR. RESULTS: In this report, we describe the set up and experimental data obtained from three different protocols (P1, P2, P3) of bilateral uterine vessel ligation in the mouse. Ligation was either performed at the cervical end of each uterine horn (P1) or at the central part of each uterine horn (P2 and P3). Time of surgery was E16 (P1), E17 (P2) or E16.5 (P3). Mortality, maternal weight and abortion parameters were recorded, as well as placentas weights, fetal resorption, viability, fetal weight and size. Results showed that P1 in test animals led to IUGR but was also accompanied with high mortality rate of mothers (50%), low viability of fetuses (8%) and high resorption rate (25%). P2 and P3 improved most of these parameters (decreased mortality and improved pregnancy outcomes; improved fetal viability to 90% and 27%, respectively) nevertheless P2 was not associated to IUGR contrary to P3. Thus P3 experimental conditions enable IUGR with better pregnancy and fetuses outcomes parameters that allow its use in experimental studies. CONCLUSIONS: Our results show that bilateral uterine artery ligation according to the protocol we have developed and validated can be used as a surgical mouse model of IUGR.
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spelling pubmed-41058742014-07-23 Bilateral uterine vessel ligation as a model of intrauterine growth restriction in mice Janot, Mathilde Cortes-Dubly, Marie-Laure Rodriguez, Stéphane Huynh-Do, Uyen Reprod Biol Endocrinol Methodology BACKGROUND: Intrauterine Growth Restriction (IUGR) occurs in up to 10% of pregnancies and is considered as a major risk to develop various diseases in adulthood, such as cardiovascular diseases, insulin resistance, hypertension or end stage kidney disease. Several IUGR models have been developed in order to understand the biological processes linked to fetal growth retardation, most of them being rat or mouse models and nutritional models. In order to reproduce altered placental flow, surgical models have also been developed, and among them bilateral uterine ligation has been frequently used. Nevertheless, this model has never been developed in the mouse, although murine tools display multiple advantages for biological research. The aim of this work was therefore to develop a mouse model of bilateral uterine ligation as a surgical model of IUGR. RESULTS: In this report, we describe the set up and experimental data obtained from three different protocols (P1, P2, P3) of bilateral uterine vessel ligation in the mouse. Ligation was either performed at the cervical end of each uterine horn (P1) or at the central part of each uterine horn (P2 and P3). Time of surgery was E16 (P1), E17 (P2) or E16.5 (P3). Mortality, maternal weight and abortion parameters were recorded, as well as placentas weights, fetal resorption, viability, fetal weight and size. Results showed that P1 in test animals led to IUGR but was also accompanied with high mortality rate of mothers (50%), low viability of fetuses (8%) and high resorption rate (25%). P2 and P3 improved most of these parameters (decreased mortality and improved pregnancy outcomes; improved fetal viability to 90% and 27%, respectively) nevertheless P2 was not associated to IUGR contrary to P3. Thus P3 experimental conditions enable IUGR with better pregnancy and fetuses outcomes parameters that allow its use in experimental studies. CONCLUSIONS: Our results show that bilateral uterine artery ligation according to the protocol we have developed and validated can be used as a surgical mouse model of IUGR. BioMed Central 2014-07-08 /pmc/articles/PMC4105874/ /pubmed/25004931 http://dx.doi.org/10.1186/1477-7827-12-62 Text en Copyright © 2014 Janot et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Methodology
Janot, Mathilde
Cortes-Dubly, Marie-Laure
Rodriguez, Stéphane
Huynh-Do, Uyen
Bilateral uterine vessel ligation as a model of intrauterine growth restriction in mice
title Bilateral uterine vessel ligation as a model of intrauterine growth restriction in mice
title_full Bilateral uterine vessel ligation as a model of intrauterine growth restriction in mice
title_fullStr Bilateral uterine vessel ligation as a model of intrauterine growth restriction in mice
title_full_unstemmed Bilateral uterine vessel ligation as a model of intrauterine growth restriction in mice
title_short Bilateral uterine vessel ligation as a model of intrauterine growth restriction in mice
title_sort bilateral uterine vessel ligation as a model of intrauterine growth restriction in mice
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4105874/
https://www.ncbi.nlm.nih.gov/pubmed/25004931
http://dx.doi.org/10.1186/1477-7827-12-62
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