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DNA interrogation by the CRISPR RNA-guided endonuclease Cas9

The CRISPR-associated enzyme Cas9 is an RNA-guided endonuclease that uses RNA:DNA base-pairing to target foreign DNA in bacteria. Cas9:guide RNA complexes are also effective genome engineering agents in animals and plants. Here we use single-molecule and bulk biochemical experiments to determine how...

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Detalles Bibliográficos
Autores principales:	Sternberg, Samuel H., Redding, Sy, Jinek, Martin, Greene, Eric C., Doudna, Jennifer A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2014
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4106473/ https://www.ncbi.nlm.nih.gov/pubmed/24476820 http://dx.doi.org/10.1038/nature13011

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