A Unique Mono- and Diacylglycerol Lipase from Penicillium cyclopium: Heterologous Expression, Biochemical Characterization and Molecular Basis for Its Substrate Selectivity

A cDNA gene encoding a mature peptide of the mono- and diacylglycerol lipase (abbreviated to PcMdl) from Penicillium cyclopium PG37 was cloned and expressed in Pichia pastoris GS115. The recombinant PcMdl (rePcMdl) with an apparent molecular weight of 39 kDa showed the highest activity (40.5 U/mL of...

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Detalles Bibliográficos
Autores principales: Tan, Zhong-Biao, Li, Jian-Fang, Li, Xue-Ting, Gu, Ying, Wu, Min-Chen, Wu, Jing, Wang, Jun-Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4106778/
https://www.ncbi.nlm.nih.gov/pubmed/25051359
http://dx.doi.org/10.1371/journal.pone.0102040

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4106778/
https://www.ncbi.nlm.nih.gov/pubmed/25051359
http://dx.doi.org/10.1371/journal.pone.0102040

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