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Expansion of IgG+ B-Cells during Mitogen Stimulation for Memory B-Cell ELISpot Analysis Is Influenced by Size and Composition of the B-Cell Pool
The memory B-cell (MBC) ELISpot assay is the main technique used to measure antigen-specific MBCs as a readout of humoral immune memory. This assay relies on the ability of MBCs to differentiate into antibody-secreting cells (ASC) upon polyclonal stimulation. The total number of IgG+ ASCs generated...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4106867/ https://www.ncbi.nlm.nih.gov/pubmed/25050555 http://dx.doi.org/10.1371/journal.pone.0102885 |