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The long head of the biceps tendon is a suitable cell source for tendon tissue regeneration

INTRODUCTION: Tendon tissue engineering (TTE) tries to produce tendinous tissue of high quality to replace dysfunctional tissue. One possible application of TTE might be the replacement of ruptured tissue of the rotator cuff. Autologous tenocytes seem to be most suitable as no differentiation in vit...

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Autores principales: Pietschmann, Matthias F., Wagenhäuser, Markus U., Gülecyüz, Mehmet F., Ficklscherer, Andreas, Jansson, Volkmar, Müller, Peter E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4107264/
https://www.ncbi.nlm.nih.gov/pubmed/25097592
http://dx.doi.org/10.5114/aoms.2014.43752
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author Pietschmann, Matthias F.
Wagenhäuser, Markus U.
Gülecyüz, Mehmet F.
Ficklscherer, Andreas
Jansson, Volkmar
Müller, Peter E.
author_facet Pietschmann, Matthias F.
Wagenhäuser, Markus U.
Gülecyüz, Mehmet F.
Ficklscherer, Andreas
Jansson, Volkmar
Müller, Peter E.
author_sort Pietschmann, Matthias F.
collection PubMed
description INTRODUCTION: Tendon tissue engineering (TTE) tries to produce tendinous tissue of high quality to replace dysfunctional tissue. One possible application of TTE might be the replacement of ruptured tissue of the rotator cuff. Autologous tenocytes seem to be most suitable as no differentiation in vitro is necessary. Today it is still uncertain if there is a difference between tendon-derived cells (TDC) of different native tissues. Moreover, the search for suitable scaffolds is another important issue in TTE. MATERIAL AND METHODS: This study compared TDC of the long head of the biceps tendon (LHB), the anterior cruciate ligament (ACL) and the tendon of the musculus semitendinosus (TMS). The TDC were isolated using the cell migration method. Cell morphology was assessed using light microscopy and gene expression was performed using polymerase chain reaction (PCR). Afterwards, cell seeding efficiency and proliferation were tested on a collagen I scaffold using the WST-1 assay. Results were confirmed using H + E staining. RESULTS: The TDC of the LHB showed higher expression levels of collagen type I and decorin (p < 0.01) compared to TDC of other origin. Results showed efficient cell seeding and proliferation within the scaffold. Proliferation within the scaffold was not as high as when cells were cultivated without a scaffold. CONCLUSIONS: The TDC of the LHB seems to be the most suitable cell source. Further research is necessary to find out if the results can be transferred to an in vivo model. The new collagen I scaffold seems to offer an opportunity to combine good biocompatibility and mechanical strength.
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spelling pubmed-41072642014-08-05 The long head of the biceps tendon is a suitable cell source for tendon tissue regeneration Pietschmann, Matthias F. Wagenhäuser, Markus U. Gülecyüz, Mehmet F. Ficklscherer, Andreas Jansson, Volkmar Müller, Peter E. Arch Med Sci Basic Research INTRODUCTION: Tendon tissue engineering (TTE) tries to produce tendinous tissue of high quality to replace dysfunctional tissue. One possible application of TTE might be the replacement of ruptured tissue of the rotator cuff. Autologous tenocytes seem to be most suitable as no differentiation in vitro is necessary. Today it is still uncertain if there is a difference between tendon-derived cells (TDC) of different native tissues. Moreover, the search for suitable scaffolds is another important issue in TTE. MATERIAL AND METHODS: This study compared TDC of the long head of the biceps tendon (LHB), the anterior cruciate ligament (ACL) and the tendon of the musculus semitendinosus (TMS). The TDC were isolated using the cell migration method. Cell morphology was assessed using light microscopy and gene expression was performed using polymerase chain reaction (PCR). Afterwards, cell seeding efficiency and proliferation were tested on a collagen I scaffold using the WST-1 assay. Results were confirmed using H + E staining. RESULTS: The TDC of the LHB showed higher expression levels of collagen type I and decorin (p < 0.01) compared to TDC of other origin. Results showed efficient cell seeding and proliferation within the scaffold. Proliferation within the scaffold was not as high as when cells were cultivated without a scaffold. CONCLUSIONS: The TDC of the LHB seems to be the most suitable cell source. Further research is necessary to find out if the results can be transferred to an in vivo model. The new collagen I scaffold seems to offer an opportunity to combine good biocompatibility and mechanical strength. Termedia Publishing House 2014-06-27 2014-06-29 /pmc/articles/PMC4107264/ /pubmed/25097592 http://dx.doi.org/10.5114/aoms.2014.43752 Text en Copyright © 2014 Termedia & Banach http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Basic Research
Pietschmann, Matthias F.
Wagenhäuser, Markus U.
Gülecyüz, Mehmet F.
Ficklscherer, Andreas
Jansson, Volkmar
Müller, Peter E.
The long head of the biceps tendon is a suitable cell source for tendon tissue regeneration
title The long head of the biceps tendon is a suitable cell source for tendon tissue regeneration
title_full The long head of the biceps tendon is a suitable cell source for tendon tissue regeneration
title_fullStr The long head of the biceps tendon is a suitable cell source for tendon tissue regeneration
title_full_unstemmed The long head of the biceps tendon is a suitable cell source for tendon tissue regeneration
title_short The long head of the biceps tendon is a suitable cell source for tendon tissue regeneration
title_sort long head of the biceps tendon is a suitable cell source for tendon tissue regeneration
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4107264/
https://www.ncbi.nlm.nih.gov/pubmed/25097592
http://dx.doi.org/10.5114/aoms.2014.43752
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