Engineered protein A ligands, derived from a histidine-scanning library, facilitate the affinity purification of IgG under mild acidic conditions

BACKGROUND: In antibody purification processes, the acidic buffer commonly used to elute the bound antibodies during conventional affinity chromatograph, can damage the antibody. Herein we describe the development of several types of affinity ligands which enable the purification of antibodies under...

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Detalles Bibliográficos
Autores principales: Tsukamoto, Masayuki, Watanabe, Hideki, Ooishi, Ayako, Honda, Shinya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4107488/
https://www.ncbi.nlm.nih.gov/pubmed/25057290
http://dx.doi.org/10.1186/1754-1611-8-15

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4107488/
https://www.ncbi.nlm.nih.gov/pubmed/25057290
http://dx.doi.org/10.1186/1754-1611-8-15

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