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Morphine Attenuates Testosterone Response to Central Injection of Kisspeptin in Male Rats
BACKGROUND: Kisspeptin and naloxone stimulate the reproductive axis while morphine inhibits its function. We have investigated the effect of central injection of kisspeptin-10 on mean plasma testosterone concentration in morphine or naloxone pretreated rats. MATERIALS AND METHODS: In this experiment...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royan Institute
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4107696/ https://www.ncbi.nlm.nih.gov/pubmed/25083187 |
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author | Mahmoudi, Fariba Khazali, Homayoun Janahmadi, Mahyar |
author_facet | Mahmoudi, Fariba Khazali, Homayoun Janahmadi, Mahyar |
author_sort | Mahmoudi, Fariba |
collection | PubMed |
description | BACKGROUND: Kisspeptin and naloxone stimulate the reproductive axis while morphine inhibits its function. We have investigated the effect of central injection of kisspeptin-10 on mean plasma testosterone concentration in morphine or naloxone pretreated rats. MATERIALS AND METHODS: In this experimental study, 60 male Wistar rats that were divid- ed into 12 groups (n=5 per group) received saline, kisspeptin (1 nmol, ICV), naloxone (2 mg/kg, subcutaneously), morphine (5 or 10 mg/kg, sc) or co-administrations of kisspeptin, morphine and naloxone at 09:00 - 09:30. In the co-administrated groups, kisspeptin was injected 15 minutes following morphine or naloxone injections. Blood samples were collected 60 minutes following injections via the tail vein. Plasma testosterone concentration was measured by a rat testosterone ELISA kit. RESULTS: Central injection of kisspeptin or subcutaneous injection of naloxone significantly increased the mean plasma testosterone concentration compared to saline while subcutaneous injections of different doses of morphine (5 or 10 mg/kg) significantly decreased testosterone compared to saline. The results revealed that morphine significantly attenuated the testosterone increase after kisspeptin injection compared to kisspeptin while a stimulatory additive effect was observed in the kisspeptin/naloxone group compared to either naloxone or kisspeptin. CONCLUSION: Morphine and kisspeptin systems may interact with each other to control the hypothalamic-pituitary-gonadal (HPG) axis. |
format | Online Article Text |
id | pubmed-4107696 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Royan Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-41076962014-07-31 Morphine Attenuates Testosterone Response to Central Injection of Kisspeptin in Male Rats Mahmoudi, Fariba Khazali, Homayoun Janahmadi, Mahyar Int J Fertil Steril Original Article BACKGROUND: Kisspeptin and naloxone stimulate the reproductive axis while morphine inhibits its function. We have investigated the effect of central injection of kisspeptin-10 on mean plasma testosterone concentration in morphine or naloxone pretreated rats. MATERIALS AND METHODS: In this experimental study, 60 male Wistar rats that were divid- ed into 12 groups (n=5 per group) received saline, kisspeptin (1 nmol, ICV), naloxone (2 mg/kg, subcutaneously), morphine (5 or 10 mg/kg, sc) or co-administrations of kisspeptin, morphine and naloxone at 09:00 - 09:30. In the co-administrated groups, kisspeptin was injected 15 minutes following morphine or naloxone injections. Blood samples were collected 60 minutes following injections via the tail vein. Plasma testosterone concentration was measured by a rat testosterone ELISA kit. RESULTS: Central injection of kisspeptin or subcutaneous injection of naloxone significantly increased the mean plasma testosterone concentration compared to saline while subcutaneous injections of different doses of morphine (5 or 10 mg/kg) significantly decreased testosterone compared to saline. The results revealed that morphine significantly attenuated the testosterone increase after kisspeptin injection compared to kisspeptin while a stimulatory additive effect was observed in the kisspeptin/naloxone group compared to either naloxone or kisspeptin. CONCLUSION: Morphine and kisspeptin systems may interact with each other to control the hypothalamic-pituitary-gonadal (HPG) axis. Royan Institute 2014 2014-07-08 /pmc/articles/PMC4107696/ /pubmed/25083187 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Mahmoudi, Fariba Khazali, Homayoun Janahmadi, Mahyar Morphine Attenuates Testosterone Response to Central Injection of Kisspeptin in Male Rats |
title | Morphine Attenuates Testosterone Response to Central
Injection of Kisspeptin in Male Rats |
title_full | Morphine Attenuates Testosterone Response to Central
Injection of Kisspeptin in Male Rats |
title_fullStr | Morphine Attenuates Testosterone Response to Central
Injection of Kisspeptin in Male Rats |
title_full_unstemmed | Morphine Attenuates Testosterone Response to Central
Injection of Kisspeptin in Male Rats |
title_short | Morphine Attenuates Testosterone Response to Central
Injection of Kisspeptin in Male Rats |
title_sort | morphine attenuates testosterone response to central
injection of kisspeptin in male rats |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4107696/ https://www.ncbi.nlm.nih.gov/pubmed/25083187 |
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