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Comparative proteomic analysis of the membrane proteins of two Haemophilus parasuis strains to identify proteins that may help in habitat adaptation and pathogenesis

BACKGROUND: Haemophilus parasuis is the causative agent of Glässer’s disease characterized by polyserositis, arthritis, and meningitis in pig, leading to serious economic loss. Despite many years of study, virulence factors and the mechanisms of the entire infection process remain largely unclear. S...

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Autores principales: Zhang, Luhua, Wen, Yiping, Li, Ying, Wei, Xingliang, Yan, Xuefeng, Wen, Xintian, Wu, Rui, Huang, Xiaobo, Huang, Yong, Yan, Qigui, Liu, Mafeng, Cao, Sanjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4107730/
https://www.ncbi.nlm.nih.gov/pubmed/25057263
http://dx.doi.org/10.1186/1477-5956-12-38
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author Zhang, Luhua
Wen, Yiping
Li, Ying
Wei, Xingliang
Yan, Xuefeng
Wen, Xintian
Wu, Rui
Huang, Xiaobo
Huang, Yong
Yan, Qigui
Liu, Mafeng
Cao, Sanjie
author_facet Zhang, Luhua
Wen, Yiping
Li, Ying
Wei, Xingliang
Yan, Xuefeng
Wen, Xintian
Wu, Rui
Huang, Xiaobo
Huang, Yong
Yan, Qigui
Liu, Mafeng
Cao, Sanjie
author_sort Zhang, Luhua
collection PubMed
description BACKGROUND: Haemophilus parasuis is the causative agent of Glässer’s disease characterized by polyserositis, arthritis, and meningitis in pig, leading to serious economic loss. Despite many years of study, virulence factors and the mechanisms of the entire infection process remain largely unclear. So two-dimensional gel electrophoresis and mass spectrometry were used to search for distinctions at the membrane protein expression level between two H. parasuis isolates aimed at uncovering some proteins potentially involved in habitat adaption and pathogenesis. RESULTS: A comparative proteomic approach combining two-dimensional gel electrophoresis with mass spectrometry and tandem mass spectrometry was employed to explore the differences among membrane proteomes of a virulent Haemophilus parasuis strain isolated from the lung of a diseased pig and an avirulent strain isolated from the nasal swab of a healthy pig. Differentially expressed protein spots identified by mass spectrometry were annotated and analyzed by bioinformatic interpretation. The mRNA level was determined by quantitative real-time PCR. Proteins representing diverse functional activities were identified. Among them, the tonB-dependent siderophore receptor was a new discovery highlighted for its activity in iron uptake. In addition, periplasmic serine protease and putrescine/spermidine ABC transporter substrate-binding protein were given focus because of their virulence potential. This study revealed that the differentially expressed proteins were important in either the habitat adaption or pathogenesis of H. parasuis. CONCLUSIONS: The outcome demonstrated the presence of some proteins which raise the speculation for their importance in helping in habitat adaption or pathogenesis within the host.
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spelling pubmed-41077302014-07-24 Comparative proteomic analysis of the membrane proteins of two Haemophilus parasuis strains to identify proteins that may help in habitat adaptation and pathogenesis Zhang, Luhua Wen, Yiping Li, Ying Wei, Xingliang Yan, Xuefeng Wen, Xintian Wu, Rui Huang, Xiaobo Huang, Yong Yan, Qigui Liu, Mafeng Cao, Sanjie Proteome Sci Research BACKGROUND: Haemophilus parasuis is the causative agent of Glässer’s disease characterized by polyserositis, arthritis, and meningitis in pig, leading to serious economic loss. Despite many years of study, virulence factors and the mechanisms of the entire infection process remain largely unclear. So two-dimensional gel electrophoresis and mass spectrometry were used to search for distinctions at the membrane protein expression level between two H. parasuis isolates aimed at uncovering some proteins potentially involved in habitat adaption and pathogenesis. RESULTS: A comparative proteomic approach combining two-dimensional gel electrophoresis with mass spectrometry and tandem mass spectrometry was employed to explore the differences among membrane proteomes of a virulent Haemophilus parasuis strain isolated from the lung of a diseased pig and an avirulent strain isolated from the nasal swab of a healthy pig. Differentially expressed protein spots identified by mass spectrometry were annotated and analyzed by bioinformatic interpretation. The mRNA level was determined by quantitative real-time PCR. Proteins representing diverse functional activities were identified. Among them, the tonB-dependent siderophore receptor was a new discovery highlighted for its activity in iron uptake. In addition, periplasmic serine protease and putrescine/spermidine ABC transporter substrate-binding protein were given focus because of their virulence potential. This study revealed that the differentially expressed proteins were important in either the habitat adaption or pathogenesis of H. parasuis. CONCLUSIONS: The outcome demonstrated the presence of some proteins which raise the speculation for their importance in helping in habitat adaption or pathogenesis within the host. BioMed Central 2014-07-07 /pmc/articles/PMC4107730/ /pubmed/25057263 http://dx.doi.org/10.1186/1477-5956-12-38 Text en Copyright © 2014 Zhang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Zhang, Luhua
Wen, Yiping
Li, Ying
Wei, Xingliang
Yan, Xuefeng
Wen, Xintian
Wu, Rui
Huang, Xiaobo
Huang, Yong
Yan, Qigui
Liu, Mafeng
Cao, Sanjie
Comparative proteomic analysis of the membrane proteins of two Haemophilus parasuis strains to identify proteins that may help in habitat adaptation and pathogenesis
title Comparative proteomic analysis of the membrane proteins of two Haemophilus parasuis strains to identify proteins that may help in habitat adaptation and pathogenesis
title_full Comparative proteomic analysis of the membrane proteins of two Haemophilus parasuis strains to identify proteins that may help in habitat adaptation and pathogenesis
title_fullStr Comparative proteomic analysis of the membrane proteins of two Haemophilus parasuis strains to identify proteins that may help in habitat adaptation and pathogenesis
title_full_unstemmed Comparative proteomic analysis of the membrane proteins of two Haemophilus parasuis strains to identify proteins that may help in habitat adaptation and pathogenesis
title_short Comparative proteomic analysis of the membrane proteins of two Haemophilus parasuis strains to identify proteins that may help in habitat adaptation and pathogenesis
title_sort comparative proteomic analysis of the membrane proteins of two haemophilus parasuis strains to identify proteins that may help in habitat adaptation and pathogenesis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4107730/
https://www.ncbi.nlm.nih.gov/pubmed/25057263
http://dx.doi.org/10.1186/1477-5956-12-38
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