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Application of sodium alginate microspheres in ischemic stroke modeling in miniature pigs☆

The miniature pig is an optimal animal model for studying nervous system disease because of its physiologic and pathologic features. However, the rete mirabile composed of arteries and veins at the skull base limits their application as a model of ischemic stroke by middle cerebral artery occlusion....

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Autores principales: Cui, Yongchun, Tian, Yi, Tang, Yue, Jia, Liujun, Wu, Aili, Peng, Peng, Yang, Jianzhong, Du, Hong, Wang, Xiaojuan, Wu, Like
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4107810/
https://www.ncbi.nlm.nih.gov/pubmed/25206443
http://dx.doi.org/10.3969/j.issn.1673-5374.2013.16.004
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author Cui, Yongchun
Tian, Yi
Tang, Yue
Jia, Liujun
Wu, Aili
Peng, Peng
Yang, Jianzhong
Du, Hong
Wang, Xiaojuan
Wu, Like
author_facet Cui, Yongchun
Tian, Yi
Tang, Yue
Jia, Liujun
Wu, Aili
Peng, Peng
Yang, Jianzhong
Du, Hong
Wang, Xiaojuan
Wu, Like
author_sort Cui, Yongchun
collection PubMed
description The miniature pig is an optimal animal model for studying nervous system disease because of its physiologic and pathologic features. However, the rete mirabile composed of arteries and veins at the skull base limits their application as a model of ischemic stroke by middle cerebral artery occlusion. The present study investigated the possibility of establishing an ischemic stroke model in the miniature pig by blocking the skull base retia with sodium alginate microspheres. Three Bama miniature pigs were used. Using the monitor of C-arm X-ray machine, sodium alginate microspheres (100–300 μm), a novel embolic material, were injected through the femoral artery, aortic arch, common carotid artery, ascending pharyngeal artery and the retia. Results were evaluated using carotid arteriography, MRI, behavior observation and histology. The unilateral rete mirabile was completely blocked, resulting in disturbance in blood supply to the basal ganglia, astasia of the right hind limb and salivation. MRI and hematoxylin-eosin staining showed an evident infarction focus in the basal ganglia. These findings indicate that sodium alginate microspheres are a suitable embolic material for blocking the skull base retia in miniature pigs to establish an ischemic stroke models.
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spelling pubmed-41078102014-09-09 Application of sodium alginate microspheres in ischemic stroke modeling in miniature pigs☆ Cui, Yongchun Tian, Yi Tang, Yue Jia, Liujun Wu, Aili Peng, Peng Yang, Jianzhong Du, Hong Wang, Xiaojuan Wu, Like Neural Regen Res Basic Research The miniature pig is an optimal animal model for studying nervous system disease because of its physiologic and pathologic features. However, the rete mirabile composed of arteries and veins at the skull base limits their application as a model of ischemic stroke by middle cerebral artery occlusion. The present study investigated the possibility of establishing an ischemic stroke model in the miniature pig by blocking the skull base retia with sodium alginate microspheres. Three Bama miniature pigs were used. Using the monitor of C-arm X-ray machine, sodium alginate microspheres (100–300 μm), a novel embolic material, were injected through the femoral artery, aortic arch, common carotid artery, ascending pharyngeal artery and the retia. Results were evaluated using carotid arteriography, MRI, behavior observation and histology. The unilateral rete mirabile was completely blocked, resulting in disturbance in blood supply to the basal ganglia, astasia of the right hind limb and salivation. MRI and hematoxylin-eosin staining showed an evident infarction focus in the basal ganglia. These findings indicate that sodium alginate microspheres are a suitable embolic material for blocking the skull base retia in miniature pigs to establish an ischemic stroke models. Medknow Publications & Media Pvt Ltd 2013-06-05 /pmc/articles/PMC4107810/ /pubmed/25206443 http://dx.doi.org/10.3969/j.issn.1673-5374.2013.16.004 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Basic Research
Cui, Yongchun
Tian, Yi
Tang, Yue
Jia, Liujun
Wu, Aili
Peng, Peng
Yang, Jianzhong
Du, Hong
Wang, Xiaojuan
Wu, Like
Application of sodium alginate microspheres in ischemic stroke modeling in miniature pigs☆
title Application of sodium alginate microspheres in ischemic stroke modeling in miniature pigs☆
title_full Application of sodium alginate microspheres in ischemic stroke modeling in miniature pigs☆
title_fullStr Application of sodium alginate microspheres in ischemic stroke modeling in miniature pigs☆
title_full_unstemmed Application of sodium alginate microspheres in ischemic stroke modeling in miniature pigs☆
title_short Application of sodium alginate microspheres in ischemic stroke modeling in miniature pigs☆
title_sort application of sodium alginate microspheres in ischemic stroke modeling in miniature pigs☆
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4107810/
https://www.ncbi.nlm.nih.gov/pubmed/25206443
http://dx.doi.org/10.3969/j.issn.1673-5374.2013.16.004
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