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An Emerging Translational Model to Screen Potential Medicinal Plants for Nephrolithiasis, an Independent Risk Factor for Chronic Kidney Disease
Pharmacological therapy for urolithiasis using medicinal plants has been increasingly adopted for the prevention of its recurrence. A Drosophila melanogaster model developed for translational research of urolithiasis was applied to evaluate agents with potential antilithic effects and calcium oxalat...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4109113/ https://www.ncbi.nlm.nih.gov/pubmed/25097661 http://dx.doi.org/10.1155/2014/972958 |
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author | Wu, San-Yuan Shen, Jui-Lung Man, Kee-Ming Lee, Yuan-Ju Chen, Huey-Yi Chen, Yung-Hsiang Tsai, Kao-Sung Tsai, Fuu-Jen Lin, Wei-Yong Chen, Wen-Chi |
author_facet | Wu, San-Yuan Shen, Jui-Lung Man, Kee-Ming Lee, Yuan-Ju Chen, Huey-Yi Chen, Yung-Hsiang Tsai, Kao-Sung Tsai, Fuu-Jen Lin, Wei-Yong Chen, Wen-Chi |
author_sort | Wu, San-Yuan |
collection | PubMed |
description | Pharmacological therapy for urolithiasis using medicinal plants has been increasingly adopted for the prevention of its recurrence. A Drosophila melanogaster model developed for translational research of urolithiasis was applied to evaluate agents with potential antilithic effects and calcium oxalate (CaOx) formation. Potential antilithic herbs were prepared in a mixture of food in a diluted concentration of 5,000 from the original extract with 0.5% ethylene glycol (EG) as the lithogenic agent. The control group was fed with food only. After 3 weeks, flies (n ≥ 150 for each group) were killed using CO(2) narcotization, and the Malpighian tubules were dissected, removed, and processed for polarized light microscopy examination of the crystals. The crystal formation rate in the EG group was 100.0%. In the study, 16 tested herbal drugs reached the crystal formation rate of 0.0%, including Salviae miltiorrhizae, Paeonia lactiflora, and Carthami flos. Scutellaria baicalensis enhanced CaOx crystal formation. Two herbal drugs Commiphora molmol and Natrii sulfas caused the death of all flies. Our rapid screening methods provided evidence that some medicinal plants have potential antilithic effects. These useful medicinal plants can be further studied using other animal or human models to verify their effects. |
format | Online Article Text |
id | pubmed-4109113 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-41091132014-08-05 An Emerging Translational Model to Screen Potential Medicinal Plants for Nephrolithiasis, an Independent Risk Factor for Chronic Kidney Disease Wu, San-Yuan Shen, Jui-Lung Man, Kee-Ming Lee, Yuan-Ju Chen, Huey-Yi Chen, Yung-Hsiang Tsai, Kao-Sung Tsai, Fuu-Jen Lin, Wei-Yong Chen, Wen-Chi Evid Based Complement Alternat Med Research Article Pharmacological therapy for urolithiasis using medicinal plants has been increasingly adopted for the prevention of its recurrence. A Drosophila melanogaster model developed for translational research of urolithiasis was applied to evaluate agents with potential antilithic effects and calcium oxalate (CaOx) formation. Potential antilithic herbs were prepared in a mixture of food in a diluted concentration of 5,000 from the original extract with 0.5% ethylene glycol (EG) as the lithogenic agent. The control group was fed with food only. After 3 weeks, flies (n ≥ 150 for each group) were killed using CO(2) narcotization, and the Malpighian tubules were dissected, removed, and processed for polarized light microscopy examination of the crystals. The crystal formation rate in the EG group was 100.0%. In the study, 16 tested herbal drugs reached the crystal formation rate of 0.0%, including Salviae miltiorrhizae, Paeonia lactiflora, and Carthami flos. Scutellaria baicalensis enhanced CaOx crystal formation. Two herbal drugs Commiphora molmol and Natrii sulfas caused the death of all flies. Our rapid screening methods provided evidence that some medicinal plants have potential antilithic effects. These useful medicinal plants can be further studied using other animal or human models to verify their effects. Hindawi Publishing Corporation 2014 2014-07-06 /pmc/articles/PMC4109113/ /pubmed/25097661 http://dx.doi.org/10.1155/2014/972958 Text en Copyright © 2014 San-Yuan Wu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wu, San-Yuan Shen, Jui-Lung Man, Kee-Ming Lee, Yuan-Ju Chen, Huey-Yi Chen, Yung-Hsiang Tsai, Kao-Sung Tsai, Fuu-Jen Lin, Wei-Yong Chen, Wen-Chi An Emerging Translational Model to Screen Potential Medicinal Plants for Nephrolithiasis, an Independent Risk Factor for Chronic Kidney Disease |
title | An Emerging Translational Model to Screen Potential Medicinal Plants for Nephrolithiasis, an Independent Risk Factor for Chronic Kidney Disease |
title_full | An Emerging Translational Model to Screen Potential Medicinal Plants for Nephrolithiasis, an Independent Risk Factor for Chronic Kidney Disease |
title_fullStr | An Emerging Translational Model to Screen Potential Medicinal Plants for Nephrolithiasis, an Independent Risk Factor for Chronic Kidney Disease |
title_full_unstemmed | An Emerging Translational Model to Screen Potential Medicinal Plants for Nephrolithiasis, an Independent Risk Factor for Chronic Kidney Disease |
title_short | An Emerging Translational Model to Screen Potential Medicinal Plants for Nephrolithiasis, an Independent Risk Factor for Chronic Kidney Disease |
title_sort | emerging translational model to screen potential medicinal plants for nephrolithiasis, an independent risk factor for chronic kidney disease |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4109113/ https://www.ncbi.nlm.nih.gov/pubmed/25097661 http://dx.doi.org/10.1155/2014/972958 |
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