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Glycosphingolipid-functionalized nanoparticles recapitulate CD169-dependent HIV-1 uptake and trafficking in dendritic cells

Ganglioside GM3, a host-derived glycosphingolipid incorporated in the membrane of HIV-1 viral particles, mediates interactions between HIV-1 and Siglec1/CD169, a protein expressed on dendritic cells (DCs). Such interactions, which seem to be independent of viral envelope glycoprotein gp120, are poor...

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Autores principales: Yu, Xinwei, Feizpour, Amin, Ramirez, Nora-Guadalupe P., Wu, Linxi, Akiyama, Hisashi, Xu, Fangda, Gummuluru, Suryaram, Reinhard, Björn M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4109413/
https://www.ncbi.nlm.nih.gov/pubmed/24947940
http://dx.doi.org/10.1038/ncomms5136
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author Yu, Xinwei
Feizpour, Amin
Ramirez, Nora-Guadalupe P.
Wu, Linxi
Akiyama, Hisashi
Xu, Fangda
Gummuluru, Suryaram
Reinhard, Björn M.
author_facet Yu, Xinwei
Feizpour, Amin
Ramirez, Nora-Guadalupe P.
Wu, Linxi
Akiyama, Hisashi
Xu, Fangda
Gummuluru, Suryaram
Reinhard, Björn M.
author_sort Yu, Xinwei
collection PubMed
description Ganglioside GM3, a host-derived glycosphingolipid incorporated in the membrane of HIV-1 viral particles, mediates interactions between HIV-1 and Siglec1/CD169, a protein expressed on dendritic cells (DCs). Such interactions, which seem to be independent of viral envelope glycoprotein gp120, are poorly understood. Here we develop a model system consisting of self-assembled artificial virus nanoparticles (AVNs) that are free of viral glycoproteins or other host-derived glycolipids and glycoproteins. These plasmonic AVNs contain a membrane of defined composition wrapped around a solid metal core. GM3-containing AVNs are captured by CD169-expressing HeLa cells or mature DCs, and are sequestered within non-lysosomal tetraspanin-positive compartments. This distribution is reminiscent of CD169-dependent HIV-1 sequestration in mature DCs. Our results highlight GM3-CD169 binding as a gp120-independent signal for sequestration and preservation of HIV-1 infectivity. They also indicate that plasmonic AVNs offer improved features over liposome-based systems and represent a versatile tool for probing specific virus-cell interactions.
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spelling pubmed-41094132014-12-20 Glycosphingolipid-functionalized nanoparticles recapitulate CD169-dependent HIV-1 uptake and trafficking in dendritic cells Yu, Xinwei Feizpour, Amin Ramirez, Nora-Guadalupe P. Wu, Linxi Akiyama, Hisashi Xu, Fangda Gummuluru, Suryaram Reinhard, Björn M. Nat Commun Article Ganglioside GM3, a host-derived glycosphingolipid incorporated in the membrane of HIV-1 viral particles, mediates interactions between HIV-1 and Siglec1/CD169, a protein expressed on dendritic cells (DCs). Such interactions, which seem to be independent of viral envelope glycoprotein gp120, are poorly understood. Here we develop a model system consisting of self-assembled artificial virus nanoparticles (AVNs) that are free of viral glycoproteins or other host-derived glycolipids and glycoproteins. These plasmonic AVNs contain a membrane of defined composition wrapped around a solid metal core. GM3-containing AVNs are captured by CD169-expressing HeLa cells or mature DCs, and are sequestered within non-lysosomal tetraspanin-positive compartments. This distribution is reminiscent of CD169-dependent HIV-1 sequestration in mature DCs. Our results highlight GM3-CD169 binding as a gp120-independent signal for sequestration and preservation of HIV-1 infectivity. They also indicate that plasmonic AVNs offer improved features over liposome-based systems and represent a versatile tool for probing specific virus-cell interactions. 2014-06-20 /pmc/articles/PMC4109413/ /pubmed/24947940 http://dx.doi.org/10.1038/ncomms5136 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Yu, Xinwei
Feizpour, Amin
Ramirez, Nora-Guadalupe P.
Wu, Linxi
Akiyama, Hisashi
Xu, Fangda
Gummuluru, Suryaram
Reinhard, Björn M.
Glycosphingolipid-functionalized nanoparticles recapitulate CD169-dependent HIV-1 uptake and trafficking in dendritic cells
title Glycosphingolipid-functionalized nanoparticles recapitulate CD169-dependent HIV-1 uptake and trafficking in dendritic cells
title_full Glycosphingolipid-functionalized nanoparticles recapitulate CD169-dependent HIV-1 uptake and trafficking in dendritic cells
title_fullStr Glycosphingolipid-functionalized nanoparticles recapitulate CD169-dependent HIV-1 uptake and trafficking in dendritic cells
title_full_unstemmed Glycosphingolipid-functionalized nanoparticles recapitulate CD169-dependent HIV-1 uptake and trafficking in dendritic cells
title_short Glycosphingolipid-functionalized nanoparticles recapitulate CD169-dependent HIV-1 uptake and trafficking in dendritic cells
title_sort glycosphingolipid-functionalized nanoparticles recapitulate cd169-dependent hiv-1 uptake and trafficking in dendritic cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4109413/
https://www.ncbi.nlm.nih.gov/pubmed/24947940
http://dx.doi.org/10.1038/ncomms5136
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