Cargando…
Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3
Whole-mount immunofluorescence to detect activated Caspase 3 (Casp3 assay) is useful to identify cells undergoing either intrinsic or extrinsic apoptosis in zebrafish embryos. The whole-mount analysis provides spatial information in regard to tissue specificity of apoptosing cells, although sectioni...
Autores principales: | Sorrells, Shelly, Toruno, Cristhian, Stewart, Rodney A., Jette, Cicely |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4109746/ https://www.ncbi.nlm.nih.gov/pubmed/24378359 http://dx.doi.org/10.3791/51060 |
Ejemplares similares
-
Interdependence of Bad and Puma during Ionizing-Radiation-Induced Apoptosis
por: Toruno, Cristhian, et al.
Publicado: (2014) -
Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization
por: Cheng, Christina N., et al.
Publicado: (2014) -
Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
por: Marra, Amanda N., et al.
Publicado: (2017) -
Spliceosomal components protect embryonic neurons from R-loop-mediated DNA damage and apoptosis
por: Sorrells, Shelly, et al.
Publicado: (2018) -
Simultaneous visualization of RNA transcripts and proteins in whole-mount mouse preimplantation embryos using single-molecule fluorescence in situ hybridization and immunofluorescence microscopy
por: Hazra, Rasmani, et al.
Publicado: (2022)