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Intrapopulation Genome Size Variation in D. melanogaster Reflects Life History Variation and Plasticity

We determined female genome sizes using flow cytometry for 211 Drosophila melanogaster sequenced inbred strains from the Drosophila Genetic Reference Panel, and found significant conspecific and intrapopulation variation in genome size. We also compared several life history traits for 25 lines with...

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Autores principales: Ellis, Lisa L., Huang, Wen, Quinn, Andrew M., Ahuja, Astha, Alfrejd, Ben, Gomez, Francisco E., Hjelmen, Carl E., Moore, Kristi L., Mackay, Trudy F. C., Johnston, J. Spencer, Tarone, Aaron M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4109859/
https://www.ncbi.nlm.nih.gov/pubmed/25057905
http://dx.doi.org/10.1371/journal.pgen.1004522
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author Ellis, Lisa L.
Huang, Wen
Quinn, Andrew M.
Ahuja, Astha
Alfrejd, Ben
Gomez, Francisco E.
Hjelmen, Carl E.
Moore, Kristi L.
Mackay, Trudy F. C.
Johnston, J. Spencer
Tarone, Aaron M.
author_facet Ellis, Lisa L.
Huang, Wen
Quinn, Andrew M.
Ahuja, Astha
Alfrejd, Ben
Gomez, Francisco E.
Hjelmen, Carl E.
Moore, Kristi L.
Mackay, Trudy F. C.
Johnston, J. Spencer
Tarone, Aaron M.
author_sort Ellis, Lisa L.
collection PubMed
description We determined female genome sizes using flow cytometry for 211 Drosophila melanogaster sequenced inbred strains from the Drosophila Genetic Reference Panel, and found significant conspecific and intrapopulation variation in genome size. We also compared several life history traits for 25 lines with large and 25 lines with small genomes in three thermal environments, and found that genome size as well as genome size by temperature interactions significantly correlated with survival to pupation and adulthood, time to pupation, female pupal mass, and female eclosion rates. Genome size accounted for up to 23% of the variation in developmental phenotypes, but the contribution of genome size to variation in life history traits was plastic and varied according to the thermal environment. Expression data implicate differences in metabolism that correspond to genome size variation. These results indicate that significant genome size variation exists within D. melanogaster and this variation may impact the evolutionary ecology of the species. Genome size variation accounts for a significant portion of life history variation in an environmentally dependent manner, suggesting that potential fitness effects associated with genome size variation also depend on environmental conditions.
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spelling pubmed-41098592014-07-29 Intrapopulation Genome Size Variation in D. melanogaster Reflects Life History Variation and Plasticity Ellis, Lisa L. Huang, Wen Quinn, Andrew M. Ahuja, Astha Alfrejd, Ben Gomez, Francisco E. Hjelmen, Carl E. Moore, Kristi L. Mackay, Trudy F. C. Johnston, J. Spencer Tarone, Aaron M. PLoS Genet Research Article We determined female genome sizes using flow cytometry for 211 Drosophila melanogaster sequenced inbred strains from the Drosophila Genetic Reference Panel, and found significant conspecific and intrapopulation variation in genome size. We also compared several life history traits for 25 lines with large and 25 lines with small genomes in three thermal environments, and found that genome size as well as genome size by temperature interactions significantly correlated with survival to pupation and adulthood, time to pupation, female pupal mass, and female eclosion rates. Genome size accounted for up to 23% of the variation in developmental phenotypes, but the contribution of genome size to variation in life history traits was plastic and varied according to the thermal environment. Expression data implicate differences in metabolism that correspond to genome size variation. These results indicate that significant genome size variation exists within D. melanogaster and this variation may impact the evolutionary ecology of the species. Genome size variation accounts for a significant portion of life history variation in an environmentally dependent manner, suggesting that potential fitness effects associated with genome size variation also depend on environmental conditions. Public Library of Science 2014-07-24 /pmc/articles/PMC4109859/ /pubmed/25057905 http://dx.doi.org/10.1371/journal.pgen.1004522 Text en © 2014 Ellis et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ellis, Lisa L.
Huang, Wen
Quinn, Andrew M.
Ahuja, Astha
Alfrejd, Ben
Gomez, Francisco E.
Hjelmen, Carl E.
Moore, Kristi L.
Mackay, Trudy F. C.
Johnston, J. Spencer
Tarone, Aaron M.
Intrapopulation Genome Size Variation in D. melanogaster Reflects Life History Variation and Plasticity
title Intrapopulation Genome Size Variation in D. melanogaster Reflects Life History Variation and Plasticity
title_full Intrapopulation Genome Size Variation in D. melanogaster Reflects Life History Variation and Plasticity
title_fullStr Intrapopulation Genome Size Variation in D. melanogaster Reflects Life History Variation and Plasticity
title_full_unstemmed Intrapopulation Genome Size Variation in D. melanogaster Reflects Life History Variation and Plasticity
title_short Intrapopulation Genome Size Variation in D. melanogaster Reflects Life History Variation and Plasticity
title_sort intrapopulation genome size variation in d. melanogaster reflects life history variation and plasticity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4109859/
https://www.ncbi.nlm.nih.gov/pubmed/25057905
http://dx.doi.org/10.1371/journal.pgen.1004522
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