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Effects of Increased CO(2) on Fish Gill and Plasma Proteome
Ocean acidification and warming are both primarily caused by increased levels of atmospheric CO(2), and marine organisms are exposed to these two stressors simultaneously. Although the effects of temperature on fish have been investigated over the last century, the long-term effects of moderate CO(2...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4109940/ https://www.ncbi.nlm.nih.gov/pubmed/25058324 http://dx.doi.org/10.1371/journal.pone.0102901 |
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author | Bresolin de Souza, Karine Jutfelt, Fredrik Kling, Peter Förlin, Lars Sturve, Joachim |
author_facet | Bresolin de Souza, Karine Jutfelt, Fredrik Kling, Peter Förlin, Lars Sturve, Joachim |
author_sort | Bresolin de Souza, Karine |
collection | PubMed |
description | Ocean acidification and warming are both primarily caused by increased levels of atmospheric CO(2), and marine organisms are exposed to these two stressors simultaneously. Although the effects of temperature on fish have been investigated over the last century, the long-term effects of moderate CO(2) exposure and the combination of both stressors are almost entirely unknown. A proteomics approach was used to assess the adverse physiological and biochemical changes that may occur from the exposure to these two environmental stressors. We analysed gills and blood plasma of Atlantic halibut (Hippoglossus hippoglossus) exposed to temperatures of 12°C (control) and 18°C (impaired growth) in combination with control (400 µatm) or high-CO(2) water (1000 µatm) for 14 weeks. The proteomic analysis was performed using two-dimensional gel electrophoresis (2DE) followed by Nanoflow LC-MS/MS using a LTQ-Orbitrap. The high-CO(2) treatment induced the up-regulation of immune system-related proteins, as indicated by the up-regulation of the plasma proteins complement component C3 and fibrinogen β chain precursor in both temperature treatments. Changes in gill proteome in the high-CO(2) (18°C) group were mostly related to increased energy metabolism proteins (ATP synthase, malate dehydrogenase, malate dehydrogenase thermostable, and fructose-1,6-bisphosphate aldolase), possibly coupled to a higher energy demand. Gills from fish exposed to high-CO(2) at both temperature treatments showed changes in proteins associated with increased cellular turnover and apoptosis signalling (annexin 5, eukaryotic translation elongation factor 1γ, receptor for protein kinase C, and putative ribosomal protein S27). This study indicates that moderate CO(2)-driven acidification, alone and combined with high temperature, can elicit biochemical changes that may affect fish health. |
format | Online Article Text |
id | pubmed-4109940 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41099402014-07-29 Effects of Increased CO(2) on Fish Gill and Plasma Proteome Bresolin de Souza, Karine Jutfelt, Fredrik Kling, Peter Förlin, Lars Sturve, Joachim PLoS One Research Article Ocean acidification and warming are both primarily caused by increased levels of atmospheric CO(2), and marine organisms are exposed to these two stressors simultaneously. Although the effects of temperature on fish have been investigated over the last century, the long-term effects of moderate CO(2) exposure and the combination of both stressors are almost entirely unknown. A proteomics approach was used to assess the adverse physiological and biochemical changes that may occur from the exposure to these two environmental stressors. We analysed gills and blood plasma of Atlantic halibut (Hippoglossus hippoglossus) exposed to temperatures of 12°C (control) and 18°C (impaired growth) in combination with control (400 µatm) or high-CO(2) water (1000 µatm) for 14 weeks. The proteomic analysis was performed using two-dimensional gel electrophoresis (2DE) followed by Nanoflow LC-MS/MS using a LTQ-Orbitrap. The high-CO(2) treatment induced the up-regulation of immune system-related proteins, as indicated by the up-regulation of the plasma proteins complement component C3 and fibrinogen β chain precursor in both temperature treatments. Changes in gill proteome in the high-CO(2) (18°C) group were mostly related to increased energy metabolism proteins (ATP synthase, malate dehydrogenase, malate dehydrogenase thermostable, and fructose-1,6-bisphosphate aldolase), possibly coupled to a higher energy demand. Gills from fish exposed to high-CO(2) at both temperature treatments showed changes in proteins associated with increased cellular turnover and apoptosis signalling (annexin 5, eukaryotic translation elongation factor 1γ, receptor for protein kinase C, and putative ribosomal protein S27). This study indicates that moderate CO(2)-driven acidification, alone and combined with high temperature, can elicit biochemical changes that may affect fish health. Public Library of Science 2014-07-24 /pmc/articles/PMC4109940/ /pubmed/25058324 http://dx.doi.org/10.1371/journal.pone.0102901 Text en © 2014 Bresolin de Souza et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Bresolin de Souza, Karine Jutfelt, Fredrik Kling, Peter Förlin, Lars Sturve, Joachim Effects of Increased CO(2) on Fish Gill and Plasma Proteome |
title | Effects of Increased CO(2) on Fish Gill and Plasma Proteome |
title_full | Effects of Increased CO(2) on Fish Gill and Plasma Proteome |
title_fullStr | Effects of Increased CO(2) on Fish Gill and Plasma Proteome |
title_full_unstemmed | Effects of Increased CO(2) on Fish Gill and Plasma Proteome |
title_short | Effects of Increased CO(2) on Fish Gill and Plasma Proteome |
title_sort | effects of increased co(2) on fish gill and plasma proteome |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4109940/ https://www.ncbi.nlm.nih.gov/pubmed/25058324 http://dx.doi.org/10.1371/journal.pone.0102901 |
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