Cargando…

Synergistic Antifungal Effect of Glabridin and Fluconazole

The incidence of invasive fungal infections is increasing in recent years. The present study mainly investigated glabridin (Gla) alone and especially in combination with fluconazole (FLC) against Cryptococcus neoformans and Candida species (Candida albicans, Candida tropicalis, Candida krusei, Candi...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Wei, Li, Li Ping, Zhang, Jun Dong, Li, Qun, Shen, Hui, Chen, Si Min, He, Li Juan, Yan, Lan, Xu, Guo Tong, An, Mao Mao, Jiang, Yuan Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4110026/
https://www.ncbi.nlm.nih.gov/pubmed/25058485
http://dx.doi.org/10.1371/journal.pone.0103442
_version_ 1782327951723855872
author Liu, Wei
Li, Li Ping
Zhang, Jun Dong
Li, Qun
Shen, Hui
Chen, Si Min
He, Li Juan
Yan, Lan
Xu, Guo Tong
An, Mao Mao
Jiang, Yuan Ying
author_facet Liu, Wei
Li, Li Ping
Zhang, Jun Dong
Li, Qun
Shen, Hui
Chen, Si Min
He, Li Juan
Yan, Lan
Xu, Guo Tong
An, Mao Mao
Jiang, Yuan Ying
author_sort Liu, Wei
collection PubMed
description The incidence of invasive fungal infections is increasing in recent years. The present study mainly investigated glabridin (Gla) alone and especially in combination with fluconazole (FLC) against Cryptococcus neoformans and Candida species (Candida albicans, Candida tropicalis, Candida krusei, Candida parapsilosis and Candida Glabratas) by different methods. The minimal inhibitory concentration (MIC) and the minimal fungicidal concentration (MFC) indicated that Gla possessed a broad-spectrum antifungal activity at relatively high concentrations. After combining with FLC, Gla exerted a potent synergistic effect against drug-resistant C. albicans and C. tropicalis at lower concentrations when interpreted by fractional inhibitory concentration index (FICI). Disk diffusion test and time-killing test confirming the synergistic fungicidal effect. Cell growth tests suggested that the synergistic effect of the two drugs depended more on the concentration of Gla. The cell envelop damage including a significant decrease of cell size and membrane permeability increasing were found after Gla treatment. Together, our results suggested that Gla possessed a synergistic effect with FLC and the cell envelope damage maybe contributed to the synergistic effect, which providing new information for developing novel antifungal agents.
format Online
Article
Text
id pubmed-4110026
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-41100262014-07-29 Synergistic Antifungal Effect of Glabridin and Fluconazole Liu, Wei Li, Li Ping Zhang, Jun Dong Li, Qun Shen, Hui Chen, Si Min He, Li Juan Yan, Lan Xu, Guo Tong An, Mao Mao Jiang, Yuan Ying PLoS One Research Article The incidence of invasive fungal infections is increasing in recent years. The present study mainly investigated glabridin (Gla) alone and especially in combination with fluconazole (FLC) against Cryptococcus neoformans and Candida species (Candida albicans, Candida tropicalis, Candida krusei, Candida parapsilosis and Candida Glabratas) by different methods. The minimal inhibitory concentration (MIC) and the minimal fungicidal concentration (MFC) indicated that Gla possessed a broad-spectrum antifungal activity at relatively high concentrations. After combining with FLC, Gla exerted a potent synergistic effect against drug-resistant C. albicans and C. tropicalis at lower concentrations when interpreted by fractional inhibitory concentration index (FICI). Disk diffusion test and time-killing test confirming the synergistic fungicidal effect. Cell growth tests suggested that the synergistic effect of the two drugs depended more on the concentration of Gla. The cell envelop damage including a significant decrease of cell size and membrane permeability increasing were found after Gla treatment. Together, our results suggested that Gla possessed a synergistic effect with FLC and the cell envelope damage maybe contributed to the synergistic effect, which providing new information for developing novel antifungal agents. Public Library of Science 2014-07-24 /pmc/articles/PMC4110026/ /pubmed/25058485 http://dx.doi.org/10.1371/journal.pone.0103442 Text en © 2014 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Liu, Wei
Li, Li Ping
Zhang, Jun Dong
Li, Qun
Shen, Hui
Chen, Si Min
He, Li Juan
Yan, Lan
Xu, Guo Tong
An, Mao Mao
Jiang, Yuan Ying
Synergistic Antifungal Effect of Glabridin and Fluconazole
title Synergistic Antifungal Effect of Glabridin and Fluconazole
title_full Synergistic Antifungal Effect of Glabridin and Fluconazole
title_fullStr Synergistic Antifungal Effect of Glabridin and Fluconazole
title_full_unstemmed Synergistic Antifungal Effect of Glabridin and Fluconazole
title_short Synergistic Antifungal Effect of Glabridin and Fluconazole
title_sort synergistic antifungal effect of glabridin and fluconazole
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4110026/
https://www.ncbi.nlm.nih.gov/pubmed/25058485
http://dx.doi.org/10.1371/journal.pone.0103442
work_keys_str_mv AT liuwei synergisticantifungaleffectofglabridinandfluconazole
AT liliping synergisticantifungaleffectofglabridinandfluconazole
AT zhangjundong synergisticantifungaleffectofglabridinandfluconazole
AT liqun synergisticantifungaleffectofglabridinandfluconazole
AT shenhui synergisticantifungaleffectofglabridinandfluconazole
AT chensimin synergisticantifungaleffectofglabridinandfluconazole
AT helijuan synergisticantifungaleffectofglabridinandfluconazole
AT yanlan synergisticantifungaleffectofglabridinandfluconazole
AT xuguotong synergisticantifungaleffectofglabridinandfluconazole
AT anmaomao synergisticantifungaleffectofglabridinandfluconazole
AT jiangyuanying synergisticantifungaleffectofglabridinandfluconazole