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Effect of sulfate on low-temperature anaerobic digestion

The effect of sulfate addition on the stability of, and microbial community behavior in, low-temperature anaerobic expanded granular sludge bed-based bioreactors was investigated at 15°C. Efficient bioreactor performance was observed, with chemical oxygen demand (COD) removal efficiencies of >90%...

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Autores principales: Madden, Pádhraig, Al-Raei, Abdul M., Enright, Anne M., Chinalia, Fabio A., de Beer, Dirk, O'Flaherty, Vincent, Collins, Gavin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4110509/
https://www.ncbi.nlm.nih.gov/pubmed/25120534
http://dx.doi.org/10.3389/fmicb.2014.00376
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author Madden, Pádhraig
Al-Raei, Abdul M.
Enright, Anne M.
Chinalia, Fabio A.
de Beer, Dirk
O'Flaherty, Vincent
Collins, Gavin
author_facet Madden, Pádhraig
Al-Raei, Abdul M.
Enright, Anne M.
Chinalia, Fabio A.
de Beer, Dirk
O'Flaherty, Vincent
Collins, Gavin
author_sort Madden, Pádhraig
collection PubMed
description The effect of sulfate addition on the stability of, and microbial community behavior in, low-temperature anaerobic expanded granular sludge bed-based bioreactors was investigated at 15°C. Efficient bioreactor performance was observed, with chemical oxygen demand (COD) removal efficiencies of >90%, and a mean SO(2−)(4) removal rate of 98.3%. In situ methanogensis appeared unaffected at a COD: SO(2−)(4) influent ratio of 8:1, and subsequently of 3:1, and was impacted marginally only when the COD: SO(2−)(4) ratio was 1:2. Specific methanogenic activity assays indicated a complex set of interactions between sulfate-reducing bacteria (SRB), methanogens and homoacetogenic bacteria. SO(2−)(4) addition resulted in predominantly acetoclastic, rather than hydrogenotrophic, methanogenesis until >600 days of SO(2−)(4)-influenced bioreactor operation. Temporal microbial community development was monitored by denaturation gradient gel electrophoresis (DGGE) of 16S rRNA genes. Fluorescence in situ hybridizations (FISH), qPCR and microsensor analysis were combined to investigate the distribution of microbial groups, and particularly SRB and methanogens, along the structure of granular biofilms. qPCR data indicated that sulfidogenic genes were present in methanogenic and sulfidogenic biofilms, indicating the potential for sulfate reduction even in bioreactors not exposed to SO(2−)(4). Although the architecture of methanogenic and sulfidogenic granules was similar, indicating the presence of SRB even in methanogenic systems, FISH with rRNA targets found that the SRB were more abundant in the sulfidogenic biofilms. Methanosaeta species were the predominant, keystone members of the archaeal community, with the complete absence of the Methanosarcina species in the experimental bioreactor by trial conclusion. Microsensor data suggested the ordered distribution of sulfate reduction and sulfide accumulation, even in methanogenic granules.
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spelling pubmed-41105092014-08-12 Effect of sulfate on low-temperature anaerobic digestion Madden, Pádhraig Al-Raei, Abdul M. Enright, Anne M. Chinalia, Fabio A. de Beer, Dirk O'Flaherty, Vincent Collins, Gavin Front Microbiol Microbiology The effect of sulfate addition on the stability of, and microbial community behavior in, low-temperature anaerobic expanded granular sludge bed-based bioreactors was investigated at 15°C. Efficient bioreactor performance was observed, with chemical oxygen demand (COD) removal efficiencies of >90%, and a mean SO(2−)(4) removal rate of 98.3%. In situ methanogensis appeared unaffected at a COD: SO(2−)(4) influent ratio of 8:1, and subsequently of 3:1, and was impacted marginally only when the COD: SO(2−)(4) ratio was 1:2. Specific methanogenic activity assays indicated a complex set of interactions between sulfate-reducing bacteria (SRB), methanogens and homoacetogenic bacteria. SO(2−)(4) addition resulted in predominantly acetoclastic, rather than hydrogenotrophic, methanogenesis until >600 days of SO(2−)(4)-influenced bioreactor operation. Temporal microbial community development was monitored by denaturation gradient gel electrophoresis (DGGE) of 16S rRNA genes. Fluorescence in situ hybridizations (FISH), qPCR and microsensor analysis were combined to investigate the distribution of microbial groups, and particularly SRB and methanogens, along the structure of granular biofilms. qPCR data indicated that sulfidogenic genes were present in methanogenic and sulfidogenic biofilms, indicating the potential for sulfate reduction even in bioreactors not exposed to SO(2−)(4). Although the architecture of methanogenic and sulfidogenic granules was similar, indicating the presence of SRB even in methanogenic systems, FISH with rRNA targets found that the SRB were more abundant in the sulfidogenic biofilms. Methanosaeta species were the predominant, keystone members of the archaeal community, with the complete absence of the Methanosarcina species in the experimental bioreactor by trial conclusion. Microsensor data suggested the ordered distribution of sulfate reduction and sulfide accumulation, even in methanogenic granules. Frontiers Media S.A. 2014-07-24 /pmc/articles/PMC4110509/ /pubmed/25120534 http://dx.doi.org/10.3389/fmicb.2014.00376 Text en Copyright © 2014 Madden, Al-Raei, Enright, Chinalia, de Beer, O'Flaherty and Collins. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Madden, Pádhraig
Al-Raei, Abdul M.
Enright, Anne M.
Chinalia, Fabio A.
de Beer, Dirk
O'Flaherty, Vincent
Collins, Gavin
Effect of sulfate on low-temperature anaerobic digestion
title Effect of sulfate on low-temperature anaerobic digestion
title_full Effect of sulfate on low-temperature anaerobic digestion
title_fullStr Effect of sulfate on low-temperature anaerobic digestion
title_full_unstemmed Effect of sulfate on low-temperature anaerobic digestion
title_short Effect of sulfate on low-temperature anaerobic digestion
title_sort effect of sulfate on low-temperature anaerobic digestion
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4110509/
https://www.ncbi.nlm.nih.gov/pubmed/25120534
http://dx.doi.org/10.3389/fmicb.2014.00376
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