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Identification, determination, and study of antioxidative activities of hesperetin and gallic acid in hydro-alcoholic extract from flowers of Eriobotrya japonica (Lindl.)
Objectives: Eriobotrya japonica belongs to the Rosaceae. Studies have shown that the flowers of this plant are rich in phenolic and flavonoid compounds. Accorrdingly, the evaluation of antioxidative effects of Eriobotrya japonica Flower Extract (EJFE) have been performed in vitro. Material and Metho...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4110785/ https://www.ncbi.nlm.nih.gov/pubmed/25068140 |
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author | Esmaeili, Amir Hossein Hajizadeh Moghaddam, Akbar Chaichi, Mohammad Javad |
author_facet | Esmaeili, Amir Hossein Hajizadeh Moghaddam, Akbar Chaichi, Mohammad Javad |
author_sort | Esmaeili, Amir Hossein |
collection | PubMed |
description | Objectives: Eriobotrya japonica belongs to the Rosaceae. Studies have shown that the flowers of this plant are rich in phenolic and flavonoid compounds. Accorrdingly, the evaluation of antioxidative effects of Eriobotrya japonica Flower Extract (EJFE) have been performed in vitro. Material and Methods: In this study, to investigate the influences of components of EJFE on its antioxidative activity, extract was prepared using hydro-alcoholic (25:75 V/V) solvent and the antioxidative activity of the extract was evaluated based on the scavenging of various radicals (DPPH and H(2)O(2)) by spectrophotometric method and chelating of ferrous ions by ferrozine reagent. Results: HPLC analysis of the Eriobotrya japonica Flower Extract (EJFE) revealed hesperetin and gallic acid as the major antioxidants. When the content of total flavonoid and polyphenolic compounds in the flower extract of this plant was examined, a significantly higher level of total polyphenols was found in Eriobotrya japonica flower extract. Conclusion: Results demonstrate that the high ability to scavenge free radicals, reducing power, and Fe(+2)chelating activity exerted by the EJFE were due to the high content of hesperetin and gallic acid in the flowers. |
format | Online Article Text |
id | pubmed-4110785 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-41107852014-07-25 Identification, determination, and study of antioxidative activities of hesperetin and gallic acid in hydro-alcoholic extract from flowers of Eriobotrya japonica (Lindl.) Esmaeili, Amir Hossein Hajizadeh Moghaddam, Akbar Chaichi, Mohammad Javad Avicenna J Phytomed Original Research Paper Objectives: Eriobotrya japonica belongs to the Rosaceae. Studies have shown that the flowers of this plant are rich in phenolic and flavonoid compounds. Accorrdingly, the evaluation of antioxidative effects of Eriobotrya japonica Flower Extract (EJFE) have been performed in vitro. Material and Methods: In this study, to investigate the influences of components of EJFE on its antioxidative activity, extract was prepared using hydro-alcoholic (25:75 V/V) solvent and the antioxidative activity of the extract was evaluated based on the scavenging of various radicals (DPPH and H(2)O(2)) by spectrophotometric method and chelating of ferrous ions by ferrozine reagent. Results: HPLC analysis of the Eriobotrya japonica Flower Extract (EJFE) revealed hesperetin and gallic acid as the major antioxidants. When the content of total flavonoid and polyphenolic compounds in the flower extract of this plant was examined, a significantly higher level of total polyphenols was found in Eriobotrya japonica flower extract. Conclusion: Results demonstrate that the high ability to scavenge free radicals, reducing power, and Fe(+2)chelating activity exerted by the EJFE were due to the high content of hesperetin and gallic acid in the flowers. Mashhad University of Medical Sciences 2014 /pmc/articles/PMC4110785/ /pubmed/25068140 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Paper Esmaeili, Amir Hossein Hajizadeh Moghaddam, Akbar Chaichi, Mohammad Javad Identification, determination, and study of antioxidative activities of hesperetin and gallic acid in hydro-alcoholic extract from flowers of Eriobotrya japonica (Lindl.) |
title | Identification, determination, and study of antioxidative activities of hesperetin and gallic acid in hydro-alcoholic extract from flowers of Eriobotrya japonica (Lindl.) |
title_full | Identification, determination, and study of antioxidative activities of hesperetin and gallic acid in hydro-alcoholic extract from flowers of Eriobotrya japonica (Lindl.) |
title_fullStr | Identification, determination, and study of antioxidative activities of hesperetin and gallic acid in hydro-alcoholic extract from flowers of Eriobotrya japonica (Lindl.) |
title_full_unstemmed | Identification, determination, and study of antioxidative activities of hesperetin and gallic acid in hydro-alcoholic extract from flowers of Eriobotrya japonica (Lindl.) |
title_short | Identification, determination, and study of antioxidative activities of hesperetin and gallic acid in hydro-alcoholic extract from flowers of Eriobotrya japonica (Lindl.) |
title_sort | identification, determination, and study of antioxidative activities of hesperetin and gallic acid in hydro-alcoholic extract from flowers of eriobotrya japonica (lindl.) |
topic | Original Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4110785/ https://www.ncbi.nlm.nih.gov/pubmed/25068140 |
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