Cargando…

A Method to Identify and Isolate Pluripotent Human Stem Cells and Mouse Epiblast Stem Cells Using Lipid Body-Associated Retinyl Ester Fluorescence

Mostrar otras versiones (1)

We describe the use of a characteristic blue fluorescence to identify and isolate pluripotent human embryonic stem cells and human-induced pluripotent stem cells. The blue fluorescence emission (450–500 nm) is readily observed by fluorescence microscopy and correlates with the expression of pluripot...

Descripción completa

Detalles Bibliográficos
Autores principales:	Muthusamy, Thangaselvam, Mukherjee, Odity, Menon, Radhika, Megha, P.B., Panicker, Mitradas M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2014
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4110788/ https://www.ncbi.nlm.nih.gov/pubmed/25068130 http://dx.doi.org/10.1016/j.stemcr.2014.05.004

Ejemplares similares

A Method to Identify and Isolate Pluripotent Human Stem Cells and Mouse Epiblast Stem Cells Using Lipid Body-Associated Retinyl Ester Fluorescence
por: Muthusamy, Thangaselvam, et al.
Publicado: (2016)

Genotoxic Effects of Culture Media on Human Pluripotent Stem Cells
por: Prakash Bangalore, Megha, et al.
Publicado: (2017)

Generation and characterization of individual-specific induced pluripotent cells from patient-derived lymphoblastoid cell lines
por: Zulfiqar, Shadaan, et al.
Publicado: (2013)

Hepatic Retinyl Ester Hydrolases and the Mobilization of Retinyl Ester Stores
por: Grumet, Lukas, et al.
Publicado: (2016)

Cripto is essential to capture mouse epiblast stem cell and human embryonic stem cell pluripotency
por: Fiorenzano, Alessandro, et al.
Publicado: (2016)

Formative pluripotent stem cells show features of epiblast cells poised for gastrulation
por: Wang, Xiaoxiao, et al.
Publicado: (2021)

Lysosomal acid lipase is the major acid retinyl ester hydrolase in cultured human hepatic stellate cells but not essential for retinyl ester degradation
por: Wagner, Carina, et al.
Publicado: (2020)

Retinyl esters form lipid droplets independently of triacylglycerol and seipin
por: Molenaar, Martijn R., et al.
Publicado: (2021)

Rat epiblast-derived stem cells recapitulate the attributes of pre-gastrulation epiblast
por: Masamsetti, V. Pragathi, et al.
Publicado: (2023)

Establishment of a primed pluripotent epiblast stem cell in FGF4-based conditions
por: Joo, Jin Young, et al.
Publicado: (2014)

Author Correction: Formative pluripotent stem cells show features of epiblast cells poised for gastrulation
por: Wang, Xiaoxiao, et al.
Publicado: (2022)

Rat post-implantation epiblast-derived pluripotent stem cells produce functional germ cells
por: Iwatsuki, Kenyu, et al.
Publicado: (2023)

Retinyl ester hydrolases and their roles in vitamin A homeostasis()
por: Schreiber, Renate, et al.
Publicado: (2012)

ERRATUM: Establishment of a primed pluripotent epiblast stem cell in FGF4-based conditions
por: Joo, Jin Young, et al.
Publicado: (2015)

Induction of Primordial Germ Cells from Pluripotent Epiblast
por: Ying, Ying, et al.
Publicado: (2002)

Cdh2 stabilizes FGFR1 and contributes to primed-state pluripotency in mouse epiblast stem cells
por: Takehara, Toshiyuki, et al.
Publicado: (2015)

Pluripotent stem cells secrete Activin A to improve their epiblast competency after injection into recipient embryos
por: Xiang, Jinzhu, et al.
Publicado: (2017)

Replacement of Retinyl Esters by Polyunsaturated Triacylglycerol Species in Lipid Droplets of Hepatic Stellate Cells during Activation
por: Testerink, Nicole, et al.
Publicado: (2012)

Detailed Characterization of Human Induced Pluripotent Stem Cells Manufactured for Therapeutic Applications
por: Baghbaderani, Behnam Ahmadian, et al.
Publicado: (2016)

Lysosomal Acid Lipase Hydrolyzes Retinyl Ester and Affects Retinoid Turnover
por: Grumet, Lukas, et al.
Publicado: (2016)

Signalling Through Retinoic Acid Receptors is Required for Reprogramming of Both Mouse Embryonic Fibroblast Cells and Epiblast Stem Cells to Induced Pluripotent Stem Cells
por: Yang, Jian, et al.
Publicado: (2015)

Generation of Mouse Parthenogenetic Epiblast Stem Cells and Their Imprinting Patterns
por: Seo, Bong Jong, et al.
Publicado: (2019)

Epiblast Ground State Is Controlled by Canonical Wnt/β-Catenin Signaling in the Postimplantation Mouse Embryo and Epiblast Stem Cells
por: Sumi, Tomoyuki, et al.
Publicado: (2013)

KIAA1363 affects retinyl ester turnover in cultured murine and human hepatic stellate cells
por: Wagner, Carina, et al.
Publicado: (2022)

A Comparison of the Rest Complex Binding Patterns in Embryonic Stem Cells and Epiblast Stem Cells
por: Seki, Masahide, et al.
Publicado: (2014)

Hepatocyte-specific deletion of lysosomal acid lipase leads to cholesteryl ester but not triglyceride or retinyl ester accumulation
por: Pajed, Laura, et al.
Publicado: (2019)

Reprogramming epiblast stem cells into pre-implantation blastocyst cell-like cells
por: Tomoda, Kiichiro, et al.
Publicado: (2021)

Noninvasive two-photon imaging reveals retinyl ester storage structures in the eye
por: Imanishi, Yoshikazu, et al.
Publicado: (2004)

An in vitro stem cell model of human epiblast and yolk sac interaction
por: Mackinlay, Kirsty ML, et al.
Publicado: (2021)

Generation and characterization of stable pig pregastrulation epiblast stem cell lines
por: Zhi, Minglei, et al.
Publicado: (2021)

AMPK activation reverts mouse epiblast stem cells to naive state
por: Liu, Yajing, et al.
Publicado: (2021)

Functional Characterization of Endothelial Cells Differentiated from Porcine Epiblast Stem Cells
por: Shin, Joon-Hong, et al.
Publicado: (2022)

Identifying the In Vivo Cellular Correlates of Antipsychotic Drugs
por: Joshi, Radhika S., et al.
Publicado: (2018)

Visualization of the Epiblast and Visceral Endodermal Cells Using Fgf5-P2A-Venus BAC Transgenic Mice and Epiblast Stem Cells
por: Khoa, Le Tran Phuc, et al.
Publicado: (2016)

Epiblast-like stem cells established by Wnt/β-catenin signaling manifest distinct features of formative pluripotency and germline competence
por: Luo, Qing, et al.
Publicado: (2023)

Epiblast cells that express MyoD recruit pluripotent cells to the skeletal muscle lineage
por: Gerhart, Jacquelyn, et al.
Publicado: (2004)

Epiblast Stem Cell-Based System Reveals Reprogramming Synergy of Germline Factors
por: Gillich, Astrid, et al.
Publicado: (2012)

E-Cadherin Promotes Incorporation of Mouse Epiblast Stem Cells into Normal Development
por: Ohtsuka, Satoshi, et al.
Publicado: (2012)

Successful Reprogramming of Epiblast Stem Cells by Blocking Nuclear Localization of β-Catenin
por: Murayama, Hideyuki, et al.
Publicado: (2014)

Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells
por: Liu, Chang, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_p8o9mnfuf4cb02slb3js4dshp3