A Method to Identify and Isolate Pluripotent Human Stem Cells and Mouse Epiblast Stem Cells Using Lipid Body-Associated Retinyl Ester Fluorescence

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We describe the use of a characteristic blue fluorescence to identify and isolate pluripotent human embryonic stem cells and human-induced pluripotent stem cells. The blue fluorescence emission (450–500 nm) is readily observed by fluorescence microscopy and correlates with the expression of pluripot...

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Detalles Bibliográficos
Autores principales: Muthusamy, Thangaselvam, Mukherjee, Odity, Menon, Radhika, Megha, P.B., Panicker, Mitradas M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4110788/
https://www.ncbi.nlm.nih.gov/pubmed/25068130
http://dx.doi.org/10.1016/j.stemcr.2014.05.004
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