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Direct Genesis of Functional Rodent and Human Schwann Cells from Skin Mesenchymal Precursors

Recent reports of directed reprogramming have raised questions about the stability of cell lineages. Here, we have addressed this issue, focusing upon skin-derived precursors (SKPs), a dermally derived precursor cell. We show by lineage tracing that murine SKPs from dorsal skin originate from mesenc...

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Autores principales: Krause, Matthew P., Dworski, Shaalee, Feinberg, Konstantin, Jones, Karen, Johnston, Adam P.W., Paul, Smitha, Paris, Maryline, Peles, Elior, Bagli, Darius, Forrest, Christopher R., Kaplan, David R., Miller, Freda D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4110792/
https://www.ncbi.nlm.nih.gov/pubmed/25068124
http://dx.doi.org/10.1016/j.stemcr.2014.05.011
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author Krause, Matthew P.
Dworski, Shaalee
Feinberg, Konstantin
Jones, Karen
Johnston, Adam P.W.
Paul, Smitha
Paris, Maryline
Peles, Elior
Bagli, Darius
Forrest, Christopher R.
Kaplan, David R.
Miller, Freda D.
author_facet Krause, Matthew P.
Dworski, Shaalee
Feinberg, Konstantin
Jones, Karen
Johnston, Adam P.W.
Paul, Smitha
Paris, Maryline
Peles, Elior
Bagli, Darius
Forrest, Christopher R.
Kaplan, David R.
Miller, Freda D.
author_sort Krause, Matthew P.
collection PubMed
description Recent reports of directed reprogramming have raised questions about the stability of cell lineages. Here, we have addressed this issue, focusing upon skin-derived precursors (SKPs), a dermally derived precursor cell. We show by lineage tracing that murine SKPs from dorsal skin originate from mesenchymal and not neural crest-derived cells. These mesenchymally derived SKPs can, without genetic manipulation, generate functional Schwann cells, a neural crest cell type, and are highly similar at the transcriptional level to Schwann cells isolated from the peripheral nerve. This is not a mouse-specific phenomenon, since human SKPs that are highly similar at the transcriptome level can be made from neural crest-derived facial and mesodermally derived foreskin dermis and the foreskin SKPs can make myelinating Schwann cells. Thus, nonneural crest-derived mesenchymal precursors can differentiate into bona fide peripheral glia in the absence of genetic manipulation, suggesting that developmentally defined lineage boundaries are more flexible than widely thought.
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spelling pubmed-41107922014-07-25 Direct Genesis of Functional Rodent and Human Schwann Cells from Skin Mesenchymal Precursors Krause, Matthew P. Dworski, Shaalee Feinberg, Konstantin Jones, Karen Johnston, Adam P.W. Paul, Smitha Paris, Maryline Peles, Elior Bagli, Darius Forrest, Christopher R. Kaplan, David R. Miller, Freda D. Stem Cell Reports Article Recent reports of directed reprogramming have raised questions about the stability of cell lineages. Here, we have addressed this issue, focusing upon skin-derived precursors (SKPs), a dermally derived precursor cell. We show by lineage tracing that murine SKPs from dorsal skin originate from mesenchymal and not neural crest-derived cells. These mesenchymally derived SKPs can, without genetic manipulation, generate functional Schwann cells, a neural crest cell type, and are highly similar at the transcriptional level to Schwann cells isolated from the peripheral nerve. This is not a mouse-specific phenomenon, since human SKPs that are highly similar at the transcriptome level can be made from neural crest-derived facial and mesodermally derived foreskin dermis and the foreskin SKPs can make myelinating Schwann cells. Thus, nonneural crest-derived mesenchymal precursors can differentiate into bona fide peripheral glia in the absence of genetic manipulation, suggesting that developmentally defined lineage boundaries are more flexible than widely thought. Elsevier 2014-06-19 /pmc/articles/PMC4110792/ /pubmed/25068124 http://dx.doi.org/10.1016/j.stemcr.2014.05.011 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Article
Krause, Matthew P.
Dworski, Shaalee
Feinberg, Konstantin
Jones, Karen
Johnston, Adam P.W.
Paul, Smitha
Paris, Maryline
Peles, Elior
Bagli, Darius
Forrest, Christopher R.
Kaplan, David R.
Miller, Freda D.
Direct Genesis of Functional Rodent and Human Schwann Cells from Skin Mesenchymal Precursors
title Direct Genesis of Functional Rodent and Human Schwann Cells from Skin Mesenchymal Precursors
title_full Direct Genesis of Functional Rodent and Human Schwann Cells from Skin Mesenchymal Precursors
title_fullStr Direct Genesis of Functional Rodent and Human Schwann Cells from Skin Mesenchymal Precursors
title_full_unstemmed Direct Genesis of Functional Rodent and Human Schwann Cells from Skin Mesenchymal Precursors
title_short Direct Genesis of Functional Rodent and Human Schwann Cells from Skin Mesenchymal Precursors
title_sort direct genesis of functional rodent and human schwann cells from skin mesenchymal precursors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4110792/
https://www.ncbi.nlm.nih.gov/pubmed/25068124
http://dx.doi.org/10.1016/j.stemcr.2014.05.011
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