Insertions in the OCL1 locus of Acinetobacter baumannii lead to shortened lipooligosaccharides

Genomes of 82 Acinetobacter baumannii global clones 1 (GC1) and 2 (GC2) isolates were sequenced and different forms of the locus predicted to direct synthesis of the outer core (OC) of the lipooligosaccharide were identified. OCL1 was in all GC2 genomes, whereas GC1 isolates carried OCL1, OCL3 or a...

Descripción completa

Detalles Bibliográficos
Autores principales: Kenyon, Johanna J., Holt, Kathryn E., Pickard, Derek, Dougan, Gordon, Hall, Ruth M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Brief Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4110982/
https://www.ncbi.nlm.nih.gov/pubmed/24861001
http://dx.doi.org/10.1016/j.resmic.2014.05.034
_version_ 1782328044762955776
author Kenyon, Johanna J.
Holt, Kathryn E.
Pickard, Derek
Dougan, Gordon
Hall, Ruth M.
author_facet Kenyon, Johanna J.
Holt, Kathryn E.
Pickard, Derek
Dougan, Gordon
Hall, Ruth M.
author_sort Kenyon, Johanna J.
collection PubMed
description Genomes of 82 Acinetobacter baumannii global clones 1 (GC1) and 2 (GC2) isolates were sequenced and different forms of the locus predicted to direct synthesis of the outer core (OC) of the lipooligosaccharide were identified. OCL1 was in all GC2 genomes, whereas GC1 isolates carried OCL1, OCL3 or a new locus, OCL5. Three mutants in which an insertion sequence (ISAba1 or ISAba23) interrupted OCL1 were identified. Isolates with OCL1 intact produced only lipooligosaccharide, while the mutants produced lipooligosaccharide of reduced molecular weight. Thus, the assignment of the OC locus as that responsible for the synthesis of the OC is correct.
format Online
Article
Text
id pubmed-4110982
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-41109822014-07-29 Insertions in the OCL1 locus of Acinetobacter baumannii lead to shortened lipooligosaccharides Kenyon, Johanna J. Holt, Kathryn E. Pickard, Derek Dougan, Gordon Hall, Ruth M. Res Microbiol Brief Note Genomes of 82 Acinetobacter baumannii global clones 1 (GC1) and 2 (GC2) isolates were sequenced and different forms of the locus predicted to direct synthesis of the outer core (OC) of the lipooligosaccharide were identified. OCL1 was in all GC2 genomes, whereas GC1 isolates carried OCL1, OCL3 or a new locus, OCL5. Three mutants in which an insertion sequence (ISAba1 or ISAba23) interrupted OCL1 were identified. Isolates with OCL1 intact produced only lipooligosaccharide, while the mutants produced lipooligosaccharide of reduced molecular weight. Thus, the assignment of the OC locus as that responsible for the synthesis of the OC is correct. Elsevier 2014-07 /pmc/articles/PMC4110982/ /pubmed/24861001 http://dx.doi.org/10.1016/j.resmic.2014.05.034 Text en © 2014 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Brief Note
Kenyon, Johanna J.
Holt, Kathryn E.
Pickard, Derek
Dougan, Gordon
Hall, Ruth M.
Insertions in the OCL1 locus of Acinetobacter baumannii lead to shortened lipooligosaccharides
title Insertions in the OCL1 locus of Acinetobacter baumannii lead to shortened lipooligosaccharides
title_full Insertions in the OCL1 locus of Acinetobacter baumannii lead to shortened lipooligosaccharides
title_fullStr Insertions in the OCL1 locus of Acinetobacter baumannii lead to shortened lipooligosaccharides
title_full_unstemmed Insertions in the OCL1 locus of Acinetobacter baumannii lead to shortened lipooligosaccharides
title_short Insertions in the OCL1 locus of Acinetobacter baumannii lead to shortened lipooligosaccharides
title_sort insertions in the ocl1 locus of acinetobacter baumannii lead to shortened lipooligosaccharides
topic Brief Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4110982/
https://www.ncbi.nlm.nih.gov/pubmed/24861001
http://dx.doi.org/10.1016/j.resmic.2014.05.034
work_keys_str_mv AT kenyonjohannaj insertionsintheocl1locusofacinetobacterbaumanniileadtoshortenedlipooligosaccharides
AT holtkathryne insertionsintheocl1locusofacinetobacterbaumanniileadtoshortenedlipooligosaccharides
AT pickardderek insertionsintheocl1locusofacinetobacterbaumanniileadtoshortenedlipooligosaccharides
AT dougangordon insertionsintheocl1locusofacinetobacterbaumanniileadtoshortenedlipooligosaccharides
AT hallruthm insertionsintheocl1locusofacinetobacterbaumanniileadtoshortenedlipooligosaccharides

Ejemplares similares