Facile Enzymatic Synthesis of Base J-Containing Oligodeoxyribonucleotides and an Analysis of the Impact of Base J on DNA Replication in Cells

We reported here the use of T4 bacteriophage β-glucosyltransferase (T4 β-GT) for the facile synthesis of base J-containing oligodeoxyribonucleotides (ODNs). We found that the enzyme could catalyze the glucosylation of 5-hydroxymethyl-2-deoxyuridine (5hmU) in both single- and double-stranded ODNs, th...

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Detalles Bibliográficos
Autores principales: Ji, Debin, Wang, Yinsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4111573/
https://www.ncbi.nlm.nih.gov/pubmed/25061973
http://dx.doi.org/10.1371/journal.pone.0103335
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author Ji, Debin
Wang, Yinsheng
author_facet Ji, Debin
Wang, Yinsheng
author_sort Ji, Debin
collection PubMed
description We reported here the use of T4 bacteriophage β-glucosyltransferase (T4 β-GT) for the facile synthesis of base J-containing oligodeoxyribonucleotides (ODNs). We found that the enzyme could catalyze the glucosylation of 5-hydroxymethyl-2-deoxyuridine (5hmU) in both single- and double-stranded ODNs, though the latter reaction occurred only when 5hmU was mispaired with a guanine. In addition, base J blocked moderately DNA replication, but it did not induce mutations during replication in human cells.
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spelling pubmed-41115732014-07-29 Facile Enzymatic Synthesis of Base J-Containing Oligodeoxyribonucleotides and an Analysis of the Impact of Base J on DNA Replication in Cells Ji, Debin Wang, Yinsheng PLoS One Research Article We reported here the use of T4 bacteriophage β-glucosyltransferase (T4 β-GT) for the facile synthesis of base J-containing oligodeoxyribonucleotides (ODNs). We found that the enzyme could catalyze the glucosylation of 5-hydroxymethyl-2-deoxyuridine (5hmU) in both single- and double-stranded ODNs, though the latter reaction occurred only when 5hmU was mispaired with a guanine. In addition, base J blocked moderately DNA replication, but it did not induce mutations during replication in human cells. Public Library of Science 2014-07-25 /pmc/articles/PMC4111573/ /pubmed/25061973 http://dx.doi.org/10.1371/journal.pone.0103335 Text en © 2014 Ji, Wang http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ji, Debin
Wang, Yinsheng
Facile Enzymatic Synthesis of Base J-Containing Oligodeoxyribonucleotides and an Analysis of the Impact of Base J on DNA Replication in Cells
title Facile Enzymatic Synthesis of Base J-Containing Oligodeoxyribonucleotides and an Analysis of the Impact of Base J on DNA Replication in Cells
title_full Facile Enzymatic Synthesis of Base J-Containing Oligodeoxyribonucleotides and an Analysis of the Impact of Base J on DNA Replication in Cells
title_fullStr Facile Enzymatic Synthesis of Base J-Containing Oligodeoxyribonucleotides and an Analysis of the Impact of Base J on DNA Replication in Cells
title_full_unstemmed Facile Enzymatic Synthesis of Base J-Containing Oligodeoxyribonucleotides and an Analysis of the Impact of Base J on DNA Replication in Cells
title_short Facile Enzymatic Synthesis of Base J-Containing Oligodeoxyribonucleotides and an Analysis of the Impact of Base J on DNA Replication in Cells
title_sort facile enzymatic synthesis of base j-containing oligodeoxyribonucleotides and an analysis of the impact of base j on dna replication in cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4111573/
https://www.ncbi.nlm.nih.gov/pubmed/25061973
http://dx.doi.org/10.1371/journal.pone.0103335
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AT wangyinsheng facileenzymaticsynthesisofbasejcontainingoligodeoxyribonucleotidesandananalysisoftheimpactofbasejondnareplicationincells

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