Cargando…

Glycerol-3-phosphate acyltransferase 4 is essential for the normal development of reproductive organs and the embryo in Brassica napus

The enzyme sn-glycerol-3-phosphate acyltransferase 4 (GPAT4) is involved in the biosynthesis of plant lipid poly-esters. The present study further characterizes the enzymatic activities of three endoplasmic reticulum-bound GPAT4 isoforms of Brassica napus and examines their roles in the development...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Xue, Chen, Guanqun, Truksa, Martin, Snyder, Crystal L., Shah, Saleh, Weselake, Randall J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4112632/
https://www.ncbi.nlm.nih.gov/pubmed/24821955
http://dx.doi.org/10.1093/jxb/eru199
_version_ 1782328195955032064
author Chen, Xue
Chen, Guanqun
Truksa, Martin
Snyder, Crystal L.
Shah, Saleh
Weselake, Randall J.
author_facet Chen, Xue
Chen, Guanqun
Truksa, Martin
Snyder, Crystal L.
Shah, Saleh
Weselake, Randall J.
author_sort Chen, Xue
collection PubMed
description The enzyme sn-glycerol-3-phosphate acyltransferase 4 (GPAT4) is involved in the biosynthesis of plant lipid poly-esters. The present study further characterizes the enzymatic activities of three endoplasmic reticulum-bound GPAT4 isoforms of Brassica napus and examines their roles in the development of reproductive organs and the embryo. All three BnGPAT4 isoforms exhibited sn-2 acyltransferase and phosphatase activities with dicarboxylic acid-CoA as acyl donor. When non-substituted acyl-CoA was used as acyl donor, the rate of acylation was considerably lower and phosphatase activity was not manifested. RNA interference (RNAi)-mediated down-regulation of all GPAT4 homologues in B. napus under the control of the napin promoter caused abnormal development of several reproductive organs and reduced seed set. Microscopic examination and reciprocal crosses revealed that both pollen grains and developing embryo sacs of the B. napus gpat4 lines were affected. The gpat4 mature embryos showed decreased cutin content and altered monomer composition. The defective embryo development further affected the oil body morphology, oil content, and fatty acid composition in gpat4 seeds. These results suggest that GPAT4 has a critical role in the development of reproductive organs and the seed of B. napus.
format Online
Article
Text
id pubmed-4112632
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-41126322014-07-31 Glycerol-3-phosphate acyltransferase 4 is essential for the normal development of reproductive organs and the embryo in Brassica napus Chen, Xue Chen, Guanqun Truksa, Martin Snyder, Crystal L. Shah, Saleh Weselake, Randall J. J Exp Bot Research Paper The enzyme sn-glycerol-3-phosphate acyltransferase 4 (GPAT4) is involved in the biosynthesis of plant lipid poly-esters. The present study further characterizes the enzymatic activities of three endoplasmic reticulum-bound GPAT4 isoforms of Brassica napus and examines their roles in the development of reproductive organs and the embryo. All three BnGPAT4 isoforms exhibited sn-2 acyltransferase and phosphatase activities with dicarboxylic acid-CoA as acyl donor. When non-substituted acyl-CoA was used as acyl donor, the rate of acylation was considerably lower and phosphatase activity was not manifested. RNA interference (RNAi)-mediated down-regulation of all GPAT4 homologues in B. napus under the control of the napin promoter caused abnormal development of several reproductive organs and reduced seed set. Microscopic examination and reciprocal crosses revealed that both pollen grains and developing embryo sacs of the B. napus gpat4 lines were affected. The gpat4 mature embryos showed decreased cutin content and altered monomer composition. The defective embryo development further affected the oil body morphology, oil content, and fatty acid composition in gpat4 seeds. These results suggest that GPAT4 has a critical role in the development of reproductive organs and the seed of B. napus. Oxford University Press 2014-08 2014-05-12 /pmc/articles/PMC4112632/ /pubmed/24821955 http://dx.doi.org/10.1093/jxb/eru199 Text en © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Chen, Xue
Chen, Guanqun
Truksa, Martin
Snyder, Crystal L.
Shah, Saleh
Weselake, Randall J.
Glycerol-3-phosphate acyltransferase 4 is essential for the normal development of reproductive organs and the embryo in Brassica napus
title Glycerol-3-phosphate acyltransferase 4 is essential for the normal development of reproductive organs and the embryo in Brassica napus
title_full Glycerol-3-phosphate acyltransferase 4 is essential for the normal development of reproductive organs and the embryo in Brassica napus
title_fullStr Glycerol-3-phosphate acyltransferase 4 is essential for the normal development of reproductive organs and the embryo in Brassica napus
title_full_unstemmed Glycerol-3-phosphate acyltransferase 4 is essential for the normal development of reproductive organs and the embryo in Brassica napus
title_short Glycerol-3-phosphate acyltransferase 4 is essential for the normal development of reproductive organs and the embryo in Brassica napus
title_sort glycerol-3-phosphate acyltransferase 4 is essential for the normal development of reproductive organs and the embryo in brassica napus
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4112632/
https://www.ncbi.nlm.nih.gov/pubmed/24821955
http://dx.doi.org/10.1093/jxb/eru199
work_keys_str_mv AT chenxue glycerol3phosphateacyltransferase4isessentialforthenormaldevelopmentofreproductiveorgansandtheembryoinbrassicanapus
AT chenguanqun glycerol3phosphateacyltransferase4isessentialforthenormaldevelopmentofreproductiveorgansandtheembryoinbrassicanapus
AT truksamartin glycerol3phosphateacyltransferase4isessentialforthenormaldevelopmentofreproductiveorgansandtheembryoinbrassicanapus
AT snydercrystall glycerol3phosphateacyltransferase4isessentialforthenormaldevelopmentofreproductiveorgansandtheembryoinbrassicanapus
AT shahsaleh glycerol3phosphateacyltransferase4isessentialforthenormaldevelopmentofreproductiveorgansandtheembryoinbrassicanapus
AT weselakerandallj glycerol3phosphateacyltransferase4isessentialforthenormaldevelopmentofreproductiveorgansandtheembryoinbrassicanapus