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Tannic acid label indicates abnormal cell development coinciding with regeneration of renal tubules
BACKGROUND: Stem/progenitor cells are in the focus of research as a future therapeutic option to stimulate regeneration in diseased renal parenchyma. However, current data indicate that successful seeding of implanted stem/progenitor cells is prevented by harmful interstitial fluid and altered extra...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4112905/ https://www.ncbi.nlm.nih.gov/pubmed/25071418 http://dx.doi.org/10.1186/1472-6890-14-34 |
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author | Minuth, Will W Denk, Lucia |
author_facet | Minuth, Will W Denk, Lucia |
author_sort | Minuth, Will W |
collection | PubMed |
description | BACKGROUND: Stem/progenitor cells are in the focus of research as a future therapeutic option to stimulate regeneration in diseased renal parenchyma. However, current data indicate that successful seeding of implanted stem/progenitor cells is prevented by harmful interstitial fluid and altered extracellular matrix. To find out possible parameters for cell adaptation, the present investigation was performed. METHODS: Renal stem/progenitor cells were mounted in an artificial interstitium for perfusion culture. Exposure to chemically defined but CO(2)-independent culture media was tested during 13 days. Cell biological features were then analyzed by histochemistry, while structural details were investigated by transmission electron microscopy after conventional and improved fixation of specimens. RESULTS: Culture of renal stem/progenitor cells as well in Leibovitz’s L-15 Medium as CO(2) Independent Medium shows in fluorescence microscopy spatial development of numerous tubules. Specimens of both media fixed by conventional glutaraldehyde exhibit in electron microscopy a homogeneous cell population in developed tubules. In contrast, fixation by glutaraldehyde including tannic acid illuminates that dispersed dark marked cells of unknown function are present. The screening further demonstrates that the dark cell type does not comply with cells found in embryonic, maturing or matured renal parenchyma. CONCLUSIONS: The actual data show that development of abnormal cell features must be taken into account, when regeneration of renal tubules is simulated under in vitro conditions. |
format | Online Article Text |
id | pubmed-4112905 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-41129052014-07-29 Tannic acid label indicates abnormal cell development coinciding with regeneration of renal tubules Minuth, Will W Denk, Lucia BMC Clin Pathol Research Article BACKGROUND: Stem/progenitor cells are in the focus of research as a future therapeutic option to stimulate regeneration in diseased renal parenchyma. However, current data indicate that successful seeding of implanted stem/progenitor cells is prevented by harmful interstitial fluid and altered extracellular matrix. To find out possible parameters for cell adaptation, the present investigation was performed. METHODS: Renal stem/progenitor cells were mounted in an artificial interstitium for perfusion culture. Exposure to chemically defined but CO(2)-independent culture media was tested during 13 days. Cell biological features were then analyzed by histochemistry, while structural details were investigated by transmission electron microscopy after conventional and improved fixation of specimens. RESULTS: Culture of renal stem/progenitor cells as well in Leibovitz’s L-15 Medium as CO(2) Independent Medium shows in fluorescence microscopy spatial development of numerous tubules. Specimens of both media fixed by conventional glutaraldehyde exhibit in electron microscopy a homogeneous cell population in developed tubules. In contrast, fixation by glutaraldehyde including tannic acid illuminates that dispersed dark marked cells of unknown function are present. The screening further demonstrates that the dark cell type does not comply with cells found in embryonic, maturing or matured renal parenchyma. CONCLUSIONS: The actual data show that development of abnormal cell features must be taken into account, when regeneration of renal tubules is simulated under in vitro conditions. BioMed Central 2014-07-15 /pmc/articles/PMC4112905/ /pubmed/25071418 http://dx.doi.org/10.1186/1472-6890-14-34 Text en Copyright © 2014 Minuth and Denk; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Minuth, Will W Denk, Lucia Tannic acid label indicates abnormal cell development coinciding with regeneration of renal tubules |
title | Tannic acid label indicates abnormal cell development coinciding with regeneration of renal tubules |
title_full | Tannic acid label indicates abnormal cell development coinciding with regeneration of renal tubules |
title_fullStr | Tannic acid label indicates abnormal cell development coinciding with regeneration of renal tubules |
title_full_unstemmed | Tannic acid label indicates abnormal cell development coinciding with regeneration of renal tubules |
title_short | Tannic acid label indicates abnormal cell development coinciding with regeneration of renal tubules |
title_sort | tannic acid label indicates abnormal cell development coinciding with regeneration of renal tubules |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4112905/ https://www.ncbi.nlm.nih.gov/pubmed/25071418 http://dx.doi.org/10.1186/1472-6890-14-34 |
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