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MicroRNA expression profile in intrauterine hypoxia-induced pulmonary hypoplasia in rats

Hypoxia is necessary for fetal development; however, excess hypoxia is detrimental. The mechanisms underlying the effects of hypoxia on lung development remain unclear, although important roles of microRNAs (miRNAs) during lung development have recently been established. However, the effect on lung...

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Autores principales: HUO, HUIYI, LUO, ZIQIANG, WANG, MINGJIE, YU, XIAOHE, LIAO, ZHENGCHANG, ZHOU, XIAOCHENG, YUE, SHAOJIE
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4113549/
https://www.ncbi.nlm.nih.gov/pubmed/25120593
http://dx.doi.org/10.3892/etm.2014.1796
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author HUO, HUIYI
LUO, ZIQIANG
WANG, MINGJIE
YU, XIAOHE
LIAO, ZHENGCHANG
ZHOU, XIAOCHENG
YUE, SHAOJIE
author_facet HUO, HUIYI
LUO, ZIQIANG
WANG, MINGJIE
YU, XIAOHE
LIAO, ZHENGCHANG
ZHOU, XIAOCHENG
YUE, SHAOJIE
author_sort HUO, HUIYI
collection PubMed
description Hypoxia is necessary for fetal development; however, excess hypoxia is detrimental. The mechanisms underlying the effects of hypoxia on lung development remain unclear, although important roles of microRNAs (miRNAs) during lung development have recently been established. However, the effect on lung development at an miRNA expression level, following changes in oxygen tension, have not yet been studied. In the present study, pregnant rats were exposed to a fraction of inspired oxygen of 10.5 or 21% for two days on gestation day 19, following which the body weight, lung wet weight, radial alveolar count (RAC) and mean linear intercept (Lm) of the newborn pups were analyzed on postnatal day 1. To define the role of miRNAs during lung development following intrauterine hypoxia exposure, the miRNA expression pattern was profiled using a miRNA microarray. The newborn rats in the hypoxic group exhibited statistically significant decreases in body weight, lung weight and the RAC, as well as a marked increase in the Lm. A total of 69 miRNAs were identified to have significant changes in expression, including 55 upregulated and 14 downregulated miRNAs. Quantitative polymerase chain reaction was used to validate the microarray results of six selected miRNAs. Therefore, the results indicated that late gestation intrauterine hypoxia exposure may cause lung injury and miRNAs may play important roles in this process.
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spelling pubmed-41135492014-08-12 MicroRNA expression profile in intrauterine hypoxia-induced pulmonary hypoplasia in rats HUO, HUIYI LUO, ZIQIANG WANG, MINGJIE YU, XIAOHE LIAO, ZHENGCHANG ZHOU, XIAOCHENG YUE, SHAOJIE Exp Ther Med Articles Hypoxia is necessary for fetal development; however, excess hypoxia is detrimental. The mechanisms underlying the effects of hypoxia on lung development remain unclear, although important roles of microRNAs (miRNAs) during lung development have recently been established. However, the effect on lung development at an miRNA expression level, following changes in oxygen tension, have not yet been studied. In the present study, pregnant rats were exposed to a fraction of inspired oxygen of 10.5 or 21% for two days on gestation day 19, following which the body weight, lung wet weight, radial alveolar count (RAC) and mean linear intercept (Lm) of the newborn pups were analyzed on postnatal day 1. To define the role of miRNAs during lung development following intrauterine hypoxia exposure, the miRNA expression pattern was profiled using a miRNA microarray. The newborn rats in the hypoxic group exhibited statistically significant decreases in body weight, lung weight and the RAC, as well as a marked increase in the Lm. A total of 69 miRNAs were identified to have significant changes in expression, including 55 upregulated and 14 downregulated miRNAs. Quantitative polymerase chain reaction was used to validate the microarray results of six selected miRNAs. Therefore, the results indicated that late gestation intrauterine hypoxia exposure may cause lung injury and miRNAs may play important roles in this process. D.A. Spandidos 2014-09 2014-06-20 /pmc/articles/PMC4113549/ /pubmed/25120593 http://dx.doi.org/10.3892/etm.2014.1796 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
HUO, HUIYI
LUO, ZIQIANG
WANG, MINGJIE
YU, XIAOHE
LIAO, ZHENGCHANG
ZHOU, XIAOCHENG
YUE, SHAOJIE
MicroRNA expression profile in intrauterine hypoxia-induced pulmonary hypoplasia in rats
title MicroRNA expression profile in intrauterine hypoxia-induced pulmonary hypoplasia in rats
title_full MicroRNA expression profile in intrauterine hypoxia-induced pulmonary hypoplasia in rats
title_fullStr MicroRNA expression profile in intrauterine hypoxia-induced pulmonary hypoplasia in rats
title_full_unstemmed MicroRNA expression profile in intrauterine hypoxia-induced pulmonary hypoplasia in rats
title_short MicroRNA expression profile in intrauterine hypoxia-induced pulmonary hypoplasia in rats
title_sort microrna expression profile in intrauterine hypoxia-induced pulmonary hypoplasia in rats
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4113549/
https://www.ncbi.nlm.nih.gov/pubmed/25120593
http://dx.doi.org/10.3892/etm.2014.1796
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