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Comparison of the radiosensitivities of neurons and glial cells derived from the same rat brain
Non-proliferating cells, such as mature neurons, are generally believed to be more resistant to X-rays than proliferating cells, such as glial and vascular endothelial cells. Therefore, the late adverse effects of radiotherapy on the brain have been attributed to the radiation-induced damage of glia...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4113551/ https://www.ncbi.nlm.nih.gov/pubmed/25120594 http://dx.doi.org/10.3892/etm.2014.1802 |
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author | KUDO, SHIGEHIRO SUZUKI, YOSHIYUKI NODA, SHIN-EI MIZUI, TOSHIYUKI SHIRAI, KATSUYUKI OKAMOTO, MASAHIKO KAMINUMA, TAKUYA YOSHIDA, YUKARI SHIRAO, TOMOAKI NAKANO, TAKASHI |
author_facet | KUDO, SHIGEHIRO SUZUKI, YOSHIYUKI NODA, SHIN-EI MIZUI, TOSHIYUKI SHIRAI, KATSUYUKI OKAMOTO, MASAHIKO KAMINUMA, TAKUYA YOSHIDA, YUKARI SHIRAO, TOMOAKI NAKANO, TAKASHI |
author_sort | KUDO, SHIGEHIRO |
collection | PubMed |
description | Non-proliferating cells, such as mature neurons, are generally believed to be more resistant to X-rays than proliferating cells, such as glial and vascular endothelial cells. Therefore, the late adverse effects of radiotherapy on the brain have been attributed to the radiation-induced damage of glial and vascular endothelial cells. However, little is known about the radiosensitivities of neurons and glial cells due to difficulties in culturing these cells, particularly neurons, independently. In the present study, primary dissociated neurons and glial cultures were prepared separately from the hippocampi and cerebrum, respectively, which had been obtained from the same fetal rat on embryonic day 18. X-irradiations of 50 Gy were performed on the cultured neurons and glial cells at 7 and 21 days in vitro (DIV). The cells were fixed at 24 h after irradiation. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling was then performed to measure the apoptotic indices (AIs). The AIs of non-irradiated and irradiated neurons at 7 DIV were 23.7±6.7 and 64.9±4.8%, and those at 21 DIV were 52.1±17.4 and 44.6±12.5%, respectively. The AIs of non-irradiated and irradiated glial cells at 7 DIV were 5.8±1.5 and 78.4±3.3% and those at 21 DIV were 9.6±2.6 and 86.3±4.9%, respectively. Glial cells and neurons were radiosensitive at 7 DIV. However, while glial cells were radiosensitive at 21 DIV, neurons were not. |
format | Online Article Text |
id | pubmed-4113551 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-41135512014-08-12 Comparison of the radiosensitivities of neurons and glial cells derived from the same rat brain KUDO, SHIGEHIRO SUZUKI, YOSHIYUKI NODA, SHIN-EI MIZUI, TOSHIYUKI SHIRAI, KATSUYUKI OKAMOTO, MASAHIKO KAMINUMA, TAKUYA YOSHIDA, YUKARI SHIRAO, TOMOAKI NAKANO, TAKASHI Exp Ther Med Articles Non-proliferating cells, such as mature neurons, are generally believed to be more resistant to X-rays than proliferating cells, such as glial and vascular endothelial cells. Therefore, the late adverse effects of radiotherapy on the brain have been attributed to the radiation-induced damage of glial and vascular endothelial cells. However, little is known about the radiosensitivities of neurons and glial cells due to difficulties in culturing these cells, particularly neurons, independently. In the present study, primary dissociated neurons and glial cultures were prepared separately from the hippocampi and cerebrum, respectively, which had been obtained from the same fetal rat on embryonic day 18. X-irradiations of 50 Gy were performed on the cultured neurons and glial cells at 7 and 21 days in vitro (DIV). The cells were fixed at 24 h after irradiation. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling was then performed to measure the apoptotic indices (AIs). The AIs of non-irradiated and irradiated neurons at 7 DIV were 23.7±6.7 and 64.9±4.8%, and those at 21 DIV were 52.1±17.4 and 44.6±12.5%, respectively. The AIs of non-irradiated and irradiated glial cells at 7 DIV were 5.8±1.5 and 78.4±3.3% and those at 21 DIV were 9.6±2.6 and 86.3±4.9%, respectively. Glial cells and neurons were radiosensitive at 7 DIV. However, while glial cells were radiosensitive at 21 DIV, neurons were not. D.A. Spandidos 2014-09 2014-06-23 /pmc/articles/PMC4113551/ /pubmed/25120594 http://dx.doi.org/10.3892/etm.2014.1802 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles KUDO, SHIGEHIRO SUZUKI, YOSHIYUKI NODA, SHIN-EI MIZUI, TOSHIYUKI SHIRAI, KATSUYUKI OKAMOTO, MASAHIKO KAMINUMA, TAKUYA YOSHIDA, YUKARI SHIRAO, TOMOAKI NAKANO, TAKASHI Comparison of the radiosensitivities of neurons and glial cells derived from the same rat brain |
title | Comparison of the radiosensitivities of neurons and glial cells derived from the same rat brain |
title_full | Comparison of the radiosensitivities of neurons and glial cells derived from the same rat brain |
title_fullStr | Comparison of the radiosensitivities of neurons and glial cells derived from the same rat brain |
title_full_unstemmed | Comparison of the radiosensitivities of neurons and glial cells derived from the same rat brain |
title_short | Comparison of the radiosensitivities of neurons and glial cells derived from the same rat brain |
title_sort | comparison of the radiosensitivities of neurons and glial cells derived from the same rat brain |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4113551/ https://www.ncbi.nlm.nih.gov/pubmed/25120594 http://dx.doi.org/10.3892/etm.2014.1802 |
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