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Wnt Signaling as a Possible Promoting Factor of Cell Differentiation in Pleomorphic Adenomas

There are well known that Wnt signaling was some roles of cell differentiation at the development tissues, especially the oral and maxillofacial regions of some developmental stages. Therefore, to determine Wnt signaling in the pleomorphic adenoma tissues, we examined. The expression of Wnt1 and β-c...

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Autores principales: Okuda, Yukiko, Nakano, Keisuke, Suzuki, Koji, Sugita, Yoshihiko, Kubo, Katsutoshi, Maeda, Hatsuhiko, Okafuji, Norimasa, Hasegawa, Hiromasa, Kawakami, Toshiyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4113590/
https://www.ncbi.nlm.nih.gov/pubmed/25076852
http://dx.doi.org/10.7150/ijms.9453
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author Okuda, Yukiko
Nakano, Keisuke
Suzuki, Koji
Sugita, Yoshihiko
Kubo, Katsutoshi
Maeda, Hatsuhiko
Okafuji, Norimasa
Hasegawa, Hiromasa
Kawakami, Toshiyuki
author_facet Okuda, Yukiko
Nakano, Keisuke
Suzuki, Koji
Sugita, Yoshihiko
Kubo, Katsutoshi
Maeda, Hatsuhiko
Okafuji, Norimasa
Hasegawa, Hiromasa
Kawakami, Toshiyuki
author_sort Okuda, Yukiko
collection PubMed
description There are well known that Wnt signaling was some roles of cell differentiation at the development tissues, especially the oral and maxillofacial regions of some developmental stages. Therefore, to determine Wnt signaling in the pleomorphic adenoma tissues, we examined. The expression of Wnt1 and β-catenin as well as the distribution of various cytoskeletal proteins CK7 and CK13 was examined in 30 cases of pleomorphic adenoma by immunohistochemistry. Wnt1 was detected in almost all tumor cells. The peripheral columnar cells in squamous metaplasia and small cuboidal cells in duct-like structures were strongly positive to Wnt1. Although β-catenin was clearly localized on the cell membrane of tumor cells, nuclear translocation was observed in small cuboidal cells and in some basaloid cells. The immunofluorescent staining pattern of Wnt1 and CK7 as well as Wnt1 and CK13 was consistent with IHC results. Thus, in pleomorphic adenoma, Wnt is involved in tumor cell differentiation of peripheral columnar cells forming solid nests and small peripheral columnar cells forming duct-like structures. Moreover, among the three currently known Wnt pathways, β-catenin is the suggested pathway working during cell differentiation. Furthermore, peripheral columnar cells in solid tumor nests and in squamous metaplasia are governed by another Wnt pathway other than β-catenin. Therefore, Wnt signaling through β-catenin pathway may be involved in the 'mixed' differentiation characteristic of pleomorphic adenoma although another pathway may also be possibly working in other parts of the tumor tissue.
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spelling pubmed-41135902014-07-30 Wnt Signaling as a Possible Promoting Factor of Cell Differentiation in Pleomorphic Adenomas Okuda, Yukiko Nakano, Keisuke Suzuki, Koji Sugita, Yoshihiko Kubo, Katsutoshi Maeda, Hatsuhiko Okafuji, Norimasa Hasegawa, Hiromasa Kawakami, Toshiyuki Int J Med Sci Research Paper There are well known that Wnt signaling was some roles of cell differentiation at the development tissues, especially the oral and maxillofacial regions of some developmental stages. Therefore, to determine Wnt signaling in the pleomorphic adenoma tissues, we examined. The expression of Wnt1 and β-catenin as well as the distribution of various cytoskeletal proteins CK7 and CK13 was examined in 30 cases of pleomorphic adenoma by immunohistochemistry. Wnt1 was detected in almost all tumor cells. The peripheral columnar cells in squamous metaplasia and small cuboidal cells in duct-like structures were strongly positive to Wnt1. Although β-catenin was clearly localized on the cell membrane of tumor cells, nuclear translocation was observed in small cuboidal cells and in some basaloid cells. The immunofluorescent staining pattern of Wnt1 and CK7 as well as Wnt1 and CK13 was consistent with IHC results. Thus, in pleomorphic adenoma, Wnt is involved in tumor cell differentiation of peripheral columnar cells forming solid nests and small peripheral columnar cells forming duct-like structures. Moreover, among the three currently known Wnt pathways, β-catenin is the suggested pathway working during cell differentiation. Furthermore, peripheral columnar cells in solid tumor nests and in squamous metaplasia are governed by another Wnt pathway other than β-catenin. Therefore, Wnt signaling through β-catenin pathway may be involved in the 'mixed' differentiation characteristic of pleomorphic adenoma although another pathway may also be possibly working in other parts of the tumor tissue. Ivyspring International Publisher 2014-07-10 /pmc/articles/PMC4113590/ /pubmed/25076852 http://dx.doi.org/10.7150/ijms.9453 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.
spellingShingle Research Paper
Okuda, Yukiko
Nakano, Keisuke
Suzuki, Koji
Sugita, Yoshihiko
Kubo, Katsutoshi
Maeda, Hatsuhiko
Okafuji, Norimasa
Hasegawa, Hiromasa
Kawakami, Toshiyuki
Wnt Signaling as a Possible Promoting Factor of Cell Differentiation in Pleomorphic Adenomas
title Wnt Signaling as a Possible Promoting Factor of Cell Differentiation in Pleomorphic Adenomas
title_full Wnt Signaling as a Possible Promoting Factor of Cell Differentiation in Pleomorphic Adenomas
title_fullStr Wnt Signaling as a Possible Promoting Factor of Cell Differentiation in Pleomorphic Adenomas
title_full_unstemmed Wnt Signaling as a Possible Promoting Factor of Cell Differentiation in Pleomorphic Adenomas
title_short Wnt Signaling as a Possible Promoting Factor of Cell Differentiation in Pleomorphic Adenomas
title_sort wnt signaling as a possible promoting factor of cell differentiation in pleomorphic adenomas
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4113590/
https://www.ncbi.nlm.nih.gov/pubmed/25076852
http://dx.doi.org/10.7150/ijms.9453
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