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Effect of sodium ferulate on delayed rectifier K(+) currents in PC12 cells

In order to investigate the effect of sodium ferulate (SF) on voltage-activated K(+) channels, the delayed rectifier K(+) current (Ik) in PC12 rat pheochromocytoma cells was recorded using the automated patch-clamp method. The results indicated that following the application of SF, the Ik in PC12 ce...

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Autores principales: WANG, WEI, WANG, YUYUN, ZHANG, CHUNLEI, SUN, MENGMENG, ZHU, XIAOYIN
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4113652/
https://www.ncbi.nlm.nih.gov/pubmed/25120634
http://dx.doi.org/10.3892/etm.2014.1806
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author WANG, WEI
WANG, YUYUN
ZHANG, CHUNLEI
SUN, MENGMENG
ZHU, XIAOYIN
author_facet WANG, WEI
WANG, YUYUN
ZHANG, CHUNLEI
SUN, MENGMENG
ZHU, XIAOYIN
author_sort WANG, WEI
collection PubMed
description In order to investigate the effect of sodium ferulate (SF) on voltage-activated K(+) channels, the delayed rectifier K(+) current (Ik) in PC12 rat pheochromocytoma cells was recorded using the automated patch-clamp method. The results indicated that following the application of SF, the Ik in PC12 cells was significantly decreased in a concentration-dependent manner. The analysis of activation kinetic curves and inactivation kinetic curves of Ik showed that SF had an effect on the activation and inactivation kinetics. Following the application of 15.3 μM SF, the activation curve of the Ik of PC12 cells was shifted to positive potentials and the inactivation curve of the Ik of PC12 cells was shifted to negative potentials. This study revealed that the delayed rectifier K(+) currents of PC12 cells were inhibited following SF treatment in a concentration-dependent manner. The mechanism may be associated with the delayed activation and enhanced inactivation of Ik-associated channels.
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spelling pubmed-41136522014-08-12 Effect of sodium ferulate on delayed rectifier K(+) currents in PC12 cells WANG, WEI WANG, YUYUN ZHANG, CHUNLEI SUN, MENGMENG ZHU, XIAOYIN Exp Ther Med Articles In order to investigate the effect of sodium ferulate (SF) on voltage-activated K(+) channels, the delayed rectifier K(+) current (Ik) in PC12 rat pheochromocytoma cells was recorded using the automated patch-clamp method. The results indicated that following the application of SF, the Ik in PC12 cells was significantly decreased in a concentration-dependent manner. The analysis of activation kinetic curves and inactivation kinetic curves of Ik showed that SF had an effect on the activation and inactivation kinetics. Following the application of 15.3 μM SF, the activation curve of the Ik of PC12 cells was shifted to positive potentials and the inactivation curve of the Ik of PC12 cells was shifted to negative potentials. This study revealed that the delayed rectifier K(+) currents of PC12 cells were inhibited following SF treatment in a concentration-dependent manner. The mechanism may be associated with the delayed activation and enhanced inactivation of Ik-associated channels. D.A. Spandidos 2014-09 2014-06-24 /pmc/articles/PMC4113652/ /pubmed/25120634 http://dx.doi.org/10.3892/etm.2014.1806 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
WANG, WEI
WANG, YUYUN
ZHANG, CHUNLEI
SUN, MENGMENG
ZHU, XIAOYIN
Effect of sodium ferulate on delayed rectifier K(+) currents in PC12 cells
title Effect of sodium ferulate on delayed rectifier K(+) currents in PC12 cells
title_full Effect of sodium ferulate on delayed rectifier K(+) currents in PC12 cells
title_fullStr Effect of sodium ferulate on delayed rectifier K(+) currents in PC12 cells
title_full_unstemmed Effect of sodium ferulate on delayed rectifier K(+) currents in PC12 cells
title_short Effect of sodium ferulate on delayed rectifier K(+) currents in PC12 cells
title_sort effect of sodium ferulate on delayed rectifier k(+) currents in pc12 cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4113652/
https://www.ncbi.nlm.nih.gov/pubmed/25120634
http://dx.doi.org/10.3892/etm.2014.1806
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