Extraction and purification of C-phycocyanin from Spirulina platensis (CCC540)

In this study a simple protocol was developed for purifying phycocyanin (PC) from Spirulina platensis (CCC540) by using ammonium sulphate precipitation, followed by a single step chromatography by using DEAE-Cellulose-11 and acetate buffer. Precipitation with 65 % ammonium sulphate resulted in 80 %...

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Detalles Bibliográficos
Autores principales: Kumar, Devendra, Dhar, Dolly Wattal, Pabbi, Sunil, Kumar, Neeraj, Walia, Suresh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer India 2014
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4113674/
https://www.ncbi.nlm.nih.gov/pubmed/25089058
http://dx.doi.org/10.1007/s40502-014-0094-7
Descripción
Sumario:In this study a simple protocol was developed for purifying phycocyanin (PC) from Spirulina platensis (CCC540) by using ammonium sulphate precipitation, followed by a single step chromatography by using DEAE-Cellulose-11 and acetate buffer. Precipitation with 65 % ammonium sulphate resulted in 80 % recovery of phycocyanin with purity of 1.5 (A(620)/A(280)). Thro1ugh chromatography an 80 % recovery of phycocyanin with a purity of 4.5 (A(620)/A(280)) was achieved. In SDS_PAGE analysis, the purified PC showed the presence of two subunit α (16 kD) and β (17 kD). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s40502-014-0094-7) contains supplementary material, which is available to authorized users.

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