Extraction and purification of C-phycocyanin from Spirulina platensis (CCC540)

In this study a simple protocol was developed for purifying phycocyanin (PC) from Spirulina platensis (CCC540) by using ammonium sulphate precipitation, followed by a single step chromatography by using DEAE-Cellulose-11 and acetate buffer. Precipitation with 65 % ammonium sulphate resulted in 80 %...

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Detalles Bibliográficos
Autores principales: Kumar, Devendra, Dhar, Dolly Wattal, Pabbi, Sunil, Kumar, Neeraj, Walia, Suresh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer India 2014
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4113674/
https://www.ncbi.nlm.nih.gov/pubmed/25089058
http://dx.doi.org/10.1007/s40502-014-0094-7
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author Kumar, Devendra
Dhar, Dolly Wattal
Pabbi, Sunil
Kumar, Neeraj
Walia, Suresh
author_facet Kumar, Devendra
Dhar, Dolly Wattal
Pabbi, Sunil
Kumar, Neeraj
Walia, Suresh
author_sort Kumar, Devendra
collection PubMed
description In this study a simple protocol was developed for purifying phycocyanin (PC) from Spirulina platensis (CCC540) by using ammonium sulphate precipitation, followed by a single step chromatography by using DEAE-Cellulose-11 and acetate buffer. Precipitation with 65 % ammonium sulphate resulted in 80 % recovery of phycocyanin with purity of 1.5 (A(620)/A(280)). Thro1ugh chromatography an 80 % recovery of phycocyanin with a purity of 4.5 (A(620)/A(280)) was achieved. In SDS_PAGE analysis, the purified PC showed the presence of two subunit α (16 kD) and β (17 kD). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s40502-014-0094-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-41136742014-07-30 Extraction and purification of C-phycocyanin from Spirulina platensis (CCC540) Kumar, Devendra Dhar, Dolly Wattal Pabbi, Sunil Kumar, Neeraj Walia, Suresh Indian J Plant Physiol Original Article In this study a simple protocol was developed for purifying phycocyanin (PC) from Spirulina platensis (CCC540) by using ammonium sulphate precipitation, followed by a single step chromatography by using DEAE-Cellulose-11 and acetate buffer. Precipitation with 65 % ammonium sulphate resulted in 80 % recovery of phycocyanin with purity of 1.5 (A(620)/A(280)). Thro1ugh chromatography an 80 % recovery of phycocyanin with a purity of 4.5 (A(620)/A(280)) was achieved. In SDS_PAGE analysis, the purified PC showed the presence of two subunit α (16 kD) and β (17 kD). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s40502-014-0094-7) contains supplementary material, which is available to authorized users. Springer India 2014-07-16 2014 /pmc/articles/PMC4113674/ /pubmed/25089058 http://dx.doi.org/10.1007/s40502-014-0094-7 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Article
Kumar, Devendra
Dhar, Dolly Wattal
Pabbi, Sunil
Kumar, Neeraj
Walia, Suresh
Extraction and purification of C-phycocyanin from Spirulina platensis (CCC540)
title Extraction and purification of C-phycocyanin from Spirulina platensis (CCC540)
title_full Extraction and purification of C-phycocyanin from Spirulina platensis (CCC540)
title_fullStr Extraction and purification of C-phycocyanin from Spirulina platensis (CCC540)
title_full_unstemmed Extraction and purification of C-phycocyanin from Spirulina platensis (CCC540)
title_short Extraction and purification of C-phycocyanin from Spirulina platensis (CCC540)
title_sort extraction and purification of c-phycocyanin from spirulina platensis (ccc540)
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4113674/
https://www.ncbi.nlm.nih.gov/pubmed/25089058
http://dx.doi.org/10.1007/s40502-014-0094-7
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