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Resveratrol Increases Nephrin and Podocin Expression and Alleviates Renal Damage in Rats Fed a High-Fat Diet

Resveratrol is well known for its anti-inflammation and anti-oxidant properties, and has been shown to be effective in alleviating the development of obesity. The purpose of this investigation was to analyze the effect of resveratrol on renal damage in obese rats induced by a high-fat diet (HFD) and...

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Autores principales: Pan, Qing-Rong, Ren, Yan-Long, Zhu, Jia-Jia, Hu, Yan-Jin, Zheng, Jin-Su, Fan, Hui, Xu, Yuan, Wang, Guang, Liu, Wen-Xian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4113760/
https://www.ncbi.nlm.nih.gov/pubmed/25025298
http://dx.doi.org/10.3390/nu6072619
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author Pan, Qing-Rong
Ren, Yan-Long
Zhu, Jia-Jia
Hu, Yan-Jin
Zheng, Jin-Su
Fan, Hui
Xu, Yuan
Wang, Guang
Liu, Wen-Xian
author_facet Pan, Qing-Rong
Ren, Yan-Long
Zhu, Jia-Jia
Hu, Yan-Jin
Zheng, Jin-Su
Fan, Hui
Xu, Yuan
Wang, Guang
Liu, Wen-Xian
author_sort Pan, Qing-Rong
collection PubMed
description Resveratrol is well known for its anti-inflammation and anti-oxidant properties, and has been shown to be effective in alleviating the development of obesity. The purpose of this investigation was to analyze the effect of resveratrol on renal damage in obese rats induced by a high-fat diet (HFD) and its possible mechanisms. Male Sprague-Dawley rats were divided into three groups: control, HFD, and HFD plus resveratrol (treated with 100 mg/kg/day resveratrol). Body weight, serum and urine metabolic parameters, and kidney histology were measured. Meanwhile, the activities of nuclear factor-κB (NF-κB) and superoxide dismutase (SOD), the content of malondialdehyde (MDA), and the protein levels of tumor necrosis factor (TNF-α), monocyte chemotactic protein-1 (MCP-1), nephrin and podocin in kidney were detected. Our work showed that resveratrol alleviated dyslipidemia and renal damage induced by HFD, decreased MDA level and increased SOD activity. Furthermore, the elevated NF-κB activity, increased TNF-α and MCP-1 levels, and reduced expressions of nephrin and podocin induced by HFD were significantly reversed by resveratrol. These results suggest resveratrol could ameliorate renal injury in rats fed a HFD, and the mechanisms are associated with suppressing oxidative stress and NF-κB signaling pathway that in turn up-regulate nephrin and podocin protein expression.
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spelling pubmed-41137602014-07-29 Resveratrol Increases Nephrin and Podocin Expression and Alleviates Renal Damage in Rats Fed a High-Fat Diet Pan, Qing-Rong Ren, Yan-Long Zhu, Jia-Jia Hu, Yan-Jin Zheng, Jin-Su Fan, Hui Xu, Yuan Wang, Guang Liu, Wen-Xian Nutrients Article Resveratrol is well known for its anti-inflammation and anti-oxidant properties, and has been shown to be effective in alleviating the development of obesity. The purpose of this investigation was to analyze the effect of resveratrol on renal damage in obese rats induced by a high-fat diet (HFD) and its possible mechanisms. Male Sprague-Dawley rats were divided into three groups: control, HFD, and HFD plus resveratrol (treated with 100 mg/kg/day resveratrol). Body weight, serum and urine metabolic parameters, and kidney histology were measured. Meanwhile, the activities of nuclear factor-κB (NF-κB) and superoxide dismutase (SOD), the content of malondialdehyde (MDA), and the protein levels of tumor necrosis factor (TNF-α), monocyte chemotactic protein-1 (MCP-1), nephrin and podocin in kidney were detected. Our work showed that resveratrol alleviated dyslipidemia and renal damage induced by HFD, decreased MDA level and increased SOD activity. Furthermore, the elevated NF-κB activity, increased TNF-α and MCP-1 levels, and reduced expressions of nephrin and podocin induced by HFD were significantly reversed by resveratrol. These results suggest resveratrol could ameliorate renal injury in rats fed a HFD, and the mechanisms are associated with suppressing oxidative stress and NF-κB signaling pathway that in turn up-regulate nephrin and podocin protein expression. MDPI 2014-07-14 /pmc/articles/PMC4113760/ /pubmed/25025298 http://dx.doi.org/10.3390/nu6072619 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Pan, Qing-Rong
Ren, Yan-Long
Zhu, Jia-Jia
Hu, Yan-Jin
Zheng, Jin-Su
Fan, Hui
Xu, Yuan
Wang, Guang
Liu, Wen-Xian
Resveratrol Increases Nephrin and Podocin Expression and Alleviates Renal Damage in Rats Fed a High-Fat Diet
title Resveratrol Increases Nephrin and Podocin Expression and Alleviates Renal Damage in Rats Fed a High-Fat Diet
title_full Resveratrol Increases Nephrin and Podocin Expression and Alleviates Renal Damage in Rats Fed a High-Fat Diet
title_fullStr Resveratrol Increases Nephrin and Podocin Expression and Alleviates Renal Damage in Rats Fed a High-Fat Diet
title_full_unstemmed Resveratrol Increases Nephrin and Podocin Expression and Alleviates Renal Damage in Rats Fed a High-Fat Diet
title_short Resveratrol Increases Nephrin and Podocin Expression and Alleviates Renal Damage in Rats Fed a High-Fat Diet
title_sort resveratrol increases nephrin and podocin expression and alleviates renal damage in rats fed a high-fat diet
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4113760/
https://www.ncbi.nlm.nih.gov/pubmed/25025298
http://dx.doi.org/10.3390/nu6072619
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