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Chemopreventive Effects of Oplopantriol A, a Novel Compound Isolated from Oplopanax horridus, on Colorectal Cancer
Oplopanax horridus is a North American botanical that has received limited investigations. We previously isolated over a dozen of the constituents from O. horridus, and among them oplopantriol A (OPT A) is a novel compound. In this study, we firstly evaluated the in vivo chemoprevention activities o...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4113763/ https://www.ncbi.nlm.nih.gov/pubmed/25045937 http://dx.doi.org/10.3390/nu6072668 |
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author | Zhang, Zhiyu Yu, Chunhao Zhang, Chun-Feng Wu, Xiao-Hui Wen, Xiao-Dong Anderson, Samantha Du, Wei Huang, Wei-Hua Li, Shao-Ping Wang, Chong-Zhi Yuan, Chun-Su |
author_facet | Zhang, Zhiyu Yu, Chunhao Zhang, Chun-Feng Wu, Xiao-Hui Wen, Xiao-Dong Anderson, Samantha Du, Wei Huang, Wei-Hua Li, Shao-Ping Wang, Chong-Zhi Yuan, Chun-Su |
author_sort | Zhang, Zhiyu |
collection | PubMed |
description | Oplopanax horridus is a North American botanical that has received limited investigations. We previously isolated over a dozen of the constituents from O. horridus, and among them oplopantriol A (OPT A) is a novel compound. In this study, we firstly evaluated the in vivo chemoprevention activities of OPT A using the xenograft colon cancer mouse model. Our data showed that this compound significantly suppressed tumor growth with dose-related effects (p < 0.01). Next, we characterized the compound’s growth inhibitory effects in human colorectal cancer cell lines HCT-116 and SW-480. With OPT A treatment, these malignant cells were significantly inhibited in both a concentration- and time-dependent manner (both p < 0.01). The IC50 was approximately 5 µM for HCT-116 and 7 µM for SW-480 cells. OPT A significantly induced apoptosis and arrested the cell cycle at the G2/M phase. From further mechanism explorations, our data showed that OPT A significantly upregulated the expression of a cluster of genes, especially the tumor necrosis factor receptor family and caspase family, suggesting that the tumor necrosis factor-related apoptotic pathway plays a key role in OPT A induced apoptosis. |
format | Online Article Text |
id | pubmed-4113763 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-41137632014-07-29 Chemopreventive Effects of Oplopantriol A, a Novel Compound Isolated from Oplopanax horridus, on Colorectal Cancer Zhang, Zhiyu Yu, Chunhao Zhang, Chun-Feng Wu, Xiao-Hui Wen, Xiao-Dong Anderson, Samantha Du, Wei Huang, Wei-Hua Li, Shao-Ping Wang, Chong-Zhi Yuan, Chun-Su Nutrients Article Oplopanax horridus is a North American botanical that has received limited investigations. We previously isolated over a dozen of the constituents from O. horridus, and among them oplopantriol A (OPT A) is a novel compound. In this study, we firstly evaluated the in vivo chemoprevention activities of OPT A using the xenograft colon cancer mouse model. Our data showed that this compound significantly suppressed tumor growth with dose-related effects (p < 0.01). Next, we characterized the compound’s growth inhibitory effects in human colorectal cancer cell lines HCT-116 and SW-480. With OPT A treatment, these malignant cells were significantly inhibited in both a concentration- and time-dependent manner (both p < 0.01). The IC50 was approximately 5 µM for HCT-116 and 7 µM for SW-480 cells. OPT A significantly induced apoptosis and arrested the cell cycle at the G2/M phase. From further mechanism explorations, our data showed that OPT A significantly upregulated the expression of a cluster of genes, especially the tumor necrosis factor receptor family and caspase family, suggesting that the tumor necrosis factor-related apoptotic pathway plays a key role in OPT A induced apoptosis. MDPI 2014-07-18 /pmc/articles/PMC4113763/ /pubmed/25045937 http://dx.doi.org/10.3390/nu6072668 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Zhang, Zhiyu Yu, Chunhao Zhang, Chun-Feng Wu, Xiao-Hui Wen, Xiao-Dong Anderson, Samantha Du, Wei Huang, Wei-Hua Li, Shao-Ping Wang, Chong-Zhi Yuan, Chun-Su Chemopreventive Effects of Oplopantriol A, a Novel Compound Isolated from Oplopanax horridus, on Colorectal Cancer |
title | Chemopreventive Effects of Oplopantriol A, a Novel Compound Isolated from Oplopanax horridus, on Colorectal Cancer |
title_full | Chemopreventive Effects of Oplopantriol A, a Novel Compound Isolated from Oplopanax horridus, on Colorectal Cancer |
title_fullStr | Chemopreventive Effects of Oplopantriol A, a Novel Compound Isolated from Oplopanax horridus, on Colorectal Cancer |
title_full_unstemmed | Chemopreventive Effects of Oplopantriol A, a Novel Compound Isolated from Oplopanax horridus, on Colorectal Cancer |
title_short | Chemopreventive Effects of Oplopantriol A, a Novel Compound Isolated from Oplopanax horridus, on Colorectal Cancer |
title_sort | chemopreventive effects of oplopantriol a, a novel compound isolated from oplopanax horridus, on colorectal cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4113763/ https://www.ncbi.nlm.nih.gov/pubmed/25045937 http://dx.doi.org/10.3390/nu6072668 |
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