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Chemopreventive Effects of Oplopantriol A, a Novel Compound Isolated from Oplopanax horridus, on Colorectal Cancer

Oplopanax horridus is a North American botanical that has received limited investigations. We previously isolated over a dozen of the constituents from O. horridus, and among them oplopantriol A (OPT A) is a novel compound. In this study, we firstly evaluated the in vivo chemoprevention activities o...

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Autores principales: Zhang, Zhiyu, Yu, Chunhao, Zhang, Chun-Feng, Wu, Xiao-Hui, Wen, Xiao-Dong, Anderson, Samantha, Du, Wei, Huang, Wei-Hua, Li, Shao-Ping, Wang, Chong-Zhi, Yuan, Chun-Su
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4113763/
https://www.ncbi.nlm.nih.gov/pubmed/25045937
http://dx.doi.org/10.3390/nu6072668
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author Zhang, Zhiyu
Yu, Chunhao
Zhang, Chun-Feng
Wu, Xiao-Hui
Wen, Xiao-Dong
Anderson, Samantha
Du, Wei
Huang, Wei-Hua
Li, Shao-Ping
Wang, Chong-Zhi
Yuan, Chun-Su
author_facet Zhang, Zhiyu
Yu, Chunhao
Zhang, Chun-Feng
Wu, Xiao-Hui
Wen, Xiao-Dong
Anderson, Samantha
Du, Wei
Huang, Wei-Hua
Li, Shao-Ping
Wang, Chong-Zhi
Yuan, Chun-Su
author_sort Zhang, Zhiyu
collection PubMed
description Oplopanax horridus is a North American botanical that has received limited investigations. We previously isolated over a dozen of the constituents from O. horridus, and among them oplopantriol A (OPT A) is a novel compound. In this study, we firstly evaluated the in vivo chemoprevention activities of OPT A using the xenograft colon cancer mouse model. Our data showed that this compound significantly suppressed tumor growth with dose-related effects (p < 0.01). Next, we characterized the compound’s growth inhibitory effects in human colorectal cancer cell lines HCT-116 and SW-480. With OPT A treatment, these malignant cells were significantly inhibited in both a concentration- and time-dependent manner (both p < 0.01). The IC50 was approximately 5 µM for HCT-116 and 7 µM for SW-480 cells. OPT A significantly induced apoptosis and arrested the cell cycle at the G2/M phase. From further mechanism explorations, our data showed that OPT A significantly upregulated the expression of a cluster of genes, especially the tumor necrosis factor receptor family and caspase family, suggesting that the tumor necrosis factor-related apoptotic pathway plays a key role in OPT A induced apoptosis.
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spelling pubmed-41137632014-07-29 Chemopreventive Effects of Oplopantriol A, a Novel Compound Isolated from Oplopanax horridus, on Colorectal Cancer Zhang, Zhiyu Yu, Chunhao Zhang, Chun-Feng Wu, Xiao-Hui Wen, Xiao-Dong Anderson, Samantha Du, Wei Huang, Wei-Hua Li, Shao-Ping Wang, Chong-Zhi Yuan, Chun-Su Nutrients Article Oplopanax horridus is a North American botanical that has received limited investigations. We previously isolated over a dozen of the constituents from O. horridus, and among them oplopantriol A (OPT A) is a novel compound. In this study, we firstly evaluated the in vivo chemoprevention activities of OPT A using the xenograft colon cancer mouse model. Our data showed that this compound significantly suppressed tumor growth with dose-related effects (p < 0.01). Next, we characterized the compound’s growth inhibitory effects in human colorectal cancer cell lines HCT-116 and SW-480. With OPT A treatment, these malignant cells were significantly inhibited in both a concentration- and time-dependent manner (both p < 0.01). The IC50 was approximately 5 µM for HCT-116 and 7 µM for SW-480 cells. OPT A significantly induced apoptosis and arrested the cell cycle at the G2/M phase. From further mechanism explorations, our data showed that OPT A significantly upregulated the expression of a cluster of genes, especially the tumor necrosis factor receptor family and caspase family, suggesting that the tumor necrosis factor-related apoptotic pathway plays a key role in OPT A induced apoptosis. MDPI 2014-07-18 /pmc/articles/PMC4113763/ /pubmed/25045937 http://dx.doi.org/10.3390/nu6072668 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Zhang, Zhiyu
Yu, Chunhao
Zhang, Chun-Feng
Wu, Xiao-Hui
Wen, Xiao-Dong
Anderson, Samantha
Du, Wei
Huang, Wei-Hua
Li, Shao-Ping
Wang, Chong-Zhi
Yuan, Chun-Su
Chemopreventive Effects of Oplopantriol A, a Novel Compound Isolated from Oplopanax horridus, on Colorectal Cancer
title Chemopreventive Effects of Oplopantriol A, a Novel Compound Isolated from Oplopanax horridus, on Colorectal Cancer
title_full Chemopreventive Effects of Oplopantriol A, a Novel Compound Isolated from Oplopanax horridus, on Colorectal Cancer
title_fullStr Chemopreventive Effects of Oplopantriol A, a Novel Compound Isolated from Oplopanax horridus, on Colorectal Cancer
title_full_unstemmed Chemopreventive Effects of Oplopantriol A, a Novel Compound Isolated from Oplopanax horridus, on Colorectal Cancer
title_short Chemopreventive Effects of Oplopantriol A, a Novel Compound Isolated from Oplopanax horridus, on Colorectal Cancer
title_sort chemopreventive effects of oplopantriol a, a novel compound isolated from oplopanax horridus, on colorectal cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4113763/
https://www.ncbi.nlm.nih.gov/pubmed/25045937
http://dx.doi.org/10.3390/nu6072668
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