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Full-Length Genome Analyses of Two New Simian Immunodeficiency Virus (SIV) Strains from Mustached Monkeys (C. Cephus) in Gabon Illustrate a Complex Evolutionary History among the SIVmus/mon/gsn Lineage

The Simian Immunodeficiency Virus (SIV) mus/mon/gsn lineage is a descendant of one of the precursor viruses to the HIV-1/SIVcpz/gor viral lineage. SIVmus and SIVgsn were sequenced from mustached and greater spot nosed monkeys in Cameroon and SIVmon from mona monkeys in Cameroon and Nigeria. In order...

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Autores principales: Liégeois, Florian, Schmidt, Fabian, Boué, Vanina, Butel, Christelle, Mouacha, Fatima, Ngari, Paul, Mve Ondo, Bertrand, Leroy, Eric, Heeney, Jonathan L., Delaporte, Eric, Peeters, Martine, Rouet, François
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4113797/
https://www.ncbi.nlm.nih.gov/pubmed/25054885
http://dx.doi.org/10.3390/v6072880
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author Liégeois, Florian
Schmidt, Fabian
Boué, Vanina
Butel, Christelle
Mouacha, Fatima
Ngari, Paul
Mve Ondo, Bertrand
Leroy, Eric
Heeney, Jonathan L.
Delaporte, Eric
Peeters, Martine
Rouet, François
author_facet Liégeois, Florian
Schmidt, Fabian
Boué, Vanina
Butel, Christelle
Mouacha, Fatima
Ngari, Paul
Mve Ondo, Bertrand
Leroy, Eric
Heeney, Jonathan L.
Delaporte, Eric
Peeters, Martine
Rouet, François
author_sort Liégeois, Florian
collection PubMed
description The Simian Immunodeficiency Virus (SIV) mus/mon/gsn lineage is a descendant of one of the precursor viruses to the HIV-1/SIVcpz/gor viral lineage. SIVmus and SIVgsn were sequenced from mustached and greater spot nosed monkeys in Cameroon and SIVmon from mona monkeys in Cameroon and Nigeria. In order to further document the genetic diversity of SIVmus, we analyzed two full-length genomes of new strains identified in Gabon. The whole genomes obtained showed the expected reading frames for gag, pol, vif, vpr, tat, rev, env, nef, and also for a vpu gene. Analyses showed that the Gabonese SIVmus strains were closely related and formed a monophyletic clade within the SIVmus/mon/gsn lineage. Nonetheless, within this lineage, the position of both new SIVmus differed according to the gene analyzed. In pol and nef gene, phylogenetic topologies suggested different evolutions for each of the two new SIVmus strains whereas in the other nucleic fragments studied, their positions fluctuated between SIVmon, SIVmus-1, and SIVgsn. In addition, in C1 domain of env, we identified an insertion of seven amino acids characteristic for the SIVmus/mon/gsn and HIV‑1/SIVcpz/SIVgor lineages. Our results show a high genetic diversity of SIVmus in mustached monkeys and suggest cross-species transmission events and recombination within SIVmus/mon/gsn lineage. Additionally, in Central Africa, hunters continue to be exposed to these simian viruses, and this represents a potential threat to humans.
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spelling pubmed-41137972014-07-29 Full-Length Genome Analyses of Two New Simian Immunodeficiency Virus (SIV) Strains from Mustached Monkeys (C. Cephus) in Gabon Illustrate a Complex Evolutionary History among the SIVmus/mon/gsn Lineage Liégeois, Florian Schmidt, Fabian Boué, Vanina Butel, Christelle Mouacha, Fatima Ngari, Paul Mve Ondo, Bertrand Leroy, Eric Heeney, Jonathan L. Delaporte, Eric Peeters, Martine Rouet, François Viruses Article The Simian Immunodeficiency Virus (SIV) mus/mon/gsn lineage is a descendant of one of the precursor viruses to the HIV-1/SIVcpz/gor viral lineage. SIVmus and SIVgsn were sequenced from mustached and greater spot nosed monkeys in Cameroon and SIVmon from mona monkeys in Cameroon and Nigeria. In order to further document the genetic diversity of SIVmus, we analyzed two full-length genomes of new strains identified in Gabon. The whole genomes obtained showed the expected reading frames for gag, pol, vif, vpr, tat, rev, env, nef, and also for a vpu gene. Analyses showed that the Gabonese SIVmus strains were closely related and formed a monophyletic clade within the SIVmus/mon/gsn lineage. Nonetheless, within this lineage, the position of both new SIVmus differed according to the gene analyzed. In pol and nef gene, phylogenetic topologies suggested different evolutions for each of the two new SIVmus strains whereas in the other nucleic fragments studied, their positions fluctuated between SIVmon, SIVmus-1, and SIVgsn. In addition, in C1 domain of env, we identified an insertion of seven amino acids characteristic for the SIVmus/mon/gsn and HIV‑1/SIVcpz/SIVgor lineages. Our results show a high genetic diversity of SIVmus in mustached monkeys and suggest cross-species transmission events and recombination within SIVmus/mon/gsn lineage. Additionally, in Central Africa, hunters continue to be exposed to these simian viruses, and this represents a potential threat to humans. MDPI 2014-07-22 /pmc/articles/PMC4113797/ /pubmed/25054885 http://dx.doi.org/10.3390/v6072880 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Liégeois, Florian
Schmidt, Fabian
Boué, Vanina
Butel, Christelle
Mouacha, Fatima
Ngari, Paul
Mve Ondo, Bertrand
Leroy, Eric
Heeney, Jonathan L.
Delaporte, Eric
Peeters, Martine
Rouet, François
Full-Length Genome Analyses of Two New Simian Immunodeficiency Virus (SIV) Strains from Mustached Monkeys (C. Cephus) in Gabon Illustrate a Complex Evolutionary History among the SIVmus/mon/gsn Lineage
title Full-Length Genome Analyses of Two New Simian Immunodeficiency Virus (SIV) Strains from Mustached Monkeys (C. Cephus) in Gabon Illustrate a Complex Evolutionary History among the SIVmus/mon/gsn Lineage
title_full Full-Length Genome Analyses of Two New Simian Immunodeficiency Virus (SIV) Strains from Mustached Monkeys (C. Cephus) in Gabon Illustrate a Complex Evolutionary History among the SIVmus/mon/gsn Lineage
title_fullStr Full-Length Genome Analyses of Two New Simian Immunodeficiency Virus (SIV) Strains from Mustached Monkeys (C. Cephus) in Gabon Illustrate a Complex Evolutionary History among the SIVmus/mon/gsn Lineage
title_full_unstemmed Full-Length Genome Analyses of Two New Simian Immunodeficiency Virus (SIV) Strains from Mustached Monkeys (C. Cephus) in Gabon Illustrate a Complex Evolutionary History among the SIVmus/mon/gsn Lineage
title_short Full-Length Genome Analyses of Two New Simian Immunodeficiency Virus (SIV) Strains from Mustached Monkeys (C. Cephus) in Gabon Illustrate a Complex Evolutionary History among the SIVmus/mon/gsn Lineage
title_sort full-length genome analyses of two new simian immunodeficiency virus (siv) strains from mustached monkeys (c. cephus) in gabon illustrate a complex evolutionary history among the sivmus/mon/gsn lineage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4113797/
https://www.ncbi.nlm.nih.gov/pubmed/25054885
http://dx.doi.org/10.3390/v6072880
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