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Pigment Cell Differentiation in Sea Urchin Blastula-Derived Primary Cell Cultures

The quinone pigments of sea urchins, specifically echinochrome and spinochromes, are known for their effective antioxidant, antibacterial, antifungal, and antitumor activities. We developed in vitro technology for inducing pigment differentiation in cell culture. The intensification of the pigment d...

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Autores principales: Ageenko, Natalya V., Kiselev, Konstantin V., Dmitrenok, Pavel S., Odintsova, Nelly A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4113803/
https://www.ncbi.nlm.nih.gov/pubmed/24979272
http://dx.doi.org/10.3390/md12073874
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author Ageenko, Natalya V.
Kiselev, Konstantin V.
Dmitrenok, Pavel S.
Odintsova, Nelly A.
author_facet Ageenko, Natalya V.
Kiselev, Konstantin V.
Dmitrenok, Pavel S.
Odintsova, Nelly A.
author_sort Ageenko, Natalya V.
collection PubMed
description The quinone pigments of sea urchins, specifically echinochrome and spinochromes, are known for their effective antioxidant, antibacterial, antifungal, and antitumor activities. We developed in vitro technology for inducing pigment differentiation in cell culture. The intensification of the pigment differentiation was accompanied by a simultaneous decrease in cell proliferation. The number of pigment cells was two-fold higher in the cells cultivated in the coelomic fluids of injured sea urchins than in those intact. The possible roles of the specific components of the coelomic fluids in the pigment differentiation process and the quantitative measurement of the production of naphthoquinone pigments during cultivation were examined by MALDI and electrospray ionization mass spectrometry. Echinochrome A and spinochrome E were produced by the cultivated cells of the sand dollar Scaphechinus mirabilis in all tested media, while only spinochromes were found in the cultivated cells of another sea urchin, Strongylocentrotus intermedius. The expression of genes associated with the induction of pigment differentiation was increased in cells cultivated in the presence of shikimic acid, a precursor of naphthoquinone pigments. Our results should contribute to the development of new techniques in marine biotechnology, including the generation of cell cultures producing complex bioactive compounds with therapeutic potential.
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spelling pubmed-41138032014-07-29 Pigment Cell Differentiation in Sea Urchin Blastula-Derived Primary Cell Cultures Ageenko, Natalya V. Kiselev, Konstantin V. Dmitrenok, Pavel S. Odintsova, Nelly A. Mar Drugs Article The quinone pigments of sea urchins, specifically echinochrome and spinochromes, are known for their effective antioxidant, antibacterial, antifungal, and antitumor activities. We developed in vitro technology for inducing pigment differentiation in cell culture. The intensification of the pigment differentiation was accompanied by a simultaneous decrease in cell proliferation. The number of pigment cells was two-fold higher in the cells cultivated in the coelomic fluids of injured sea urchins than in those intact. The possible roles of the specific components of the coelomic fluids in the pigment differentiation process and the quantitative measurement of the production of naphthoquinone pigments during cultivation were examined by MALDI and electrospray ionization mass spectrometry. Echinochrome A and spinochrome E were produced by the cultivated cells of the sand dollar Scaphechinus mirabilis in all tested media, while only spinochromes were found in the cultivated cells of another sea urchin, Strongylocentrotus intermedius. The expression of genes associated with the induction of pigment differentiation was increased in cells cultivated in the presence of shikimic acid, a precursor of naphthoquinone pigments. Our results should contribute to the development of new techniques in marine biotechnology, including the generation of cell cultures producing complex bioactive compounds with therapeutic potential. MDPI 2014-06-27 /pmc/articles/PMC4113803/ /pubmed/24979272 http://dx.doi.org/10.3390/md12073874 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Ageenko, Natalya V.
Kiselev, Konstantin V.
Dmitrenok, Pavel S.
Odintsova, Nelly A.
Pigment Cell Differentiation in Sea Urchin Blastula-Derived Primary Cell Cultures
title Pigment Cell Differentiation in Sea Urchin Blastula-Derived Primary Cell Cultures
title_full Pigment Cell Differentiation in Sea Urchin Blastula-Derived Primary Cell Cultures
title_fullStr Pigment Cell Differentiation in Sea Urchin Blastula-Derived Primary Cell Cultures
title_full_unstemmed Pigment Cell Differentiation in Sea Urchin Blastula-Derived Primary Cell Cultures
title_short Pigment Cell Differentiation in Sea Urchin Blastula-Derived Primary Cell Cultures
title_sort pigment cell differentiation in sea urchin blastula-derived primary cell cultures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4113803/
https://www.ncbi.nlm.nih.gov/pubmed/24979272
http://dx.doi.org/10.3390/md12073874
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